Molecular cloning and expression of a glycosaminoglycan N- acetylglucosaminyl N-deacetylase/N-sulfotransferase from a heparin-producing cell line

A. Orellana, C. B. Hirschberg, Z. Wei, S. J. Swiedler, M. Ishihara

Resultado de la investigación: Contribución a una revistaArtículo

124 Citas (Scopus)

Resumen

Heparin has a higher content of N-sulfated glucosamine and L-iduronic acid than heparan sulfate. Deacetylation of N-acetylglucosamine followed by N- sulfation may be important steps differentiating the biosynthesis of these glycosaminoglycans. We have cloned, by cross-hybridization with the cDNA from rat liver heparan sulfate N-deacetylase/N-sulfotransferase, a protein from a heparin synthesizing mastocytoma derived cell line called MST. This protein, which has both N-deacetylase/N-sulfotransferase activities, has a predicted amino acid sequence homology of 70% with the above rat liver enzyme and is unique for the following reasons. 1) It was found to be encoded by a 3.8- kilobase mRNA that was unique to heparin-producing cells; an 8.5-kilobase mRNA encoding the rat liver enzymes has been found to occur in all mammalian cells tested on the basis of nucleic acid cross-hybridization; 2) the protein overexpressed in COS cells in its full-length transmembrane form or as a soluble secreted protein A chimera displayed ratios of N-deacetylase to N- sulfotransferase activities that were 4-8-fold higher than that observed for the enzyme found in liver that is involved in the biosynthesis of heparan sulfate. These results suggest that the MST-derived enzyme is probably unique to the production of heparin in mast cells.

Idioma originalInglés
Páginas (desde-hasta)2270-2276
Número de páginas7
PublicaciónJournal of Biological Chemistry
Volumen269
N.º3
EstadoPublicada - 1994

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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