Molecular characterization of a novel Na+/H+ antiporter cDNA from Eucalyptus globulus

Fabiola Baltierra, Mabel Castillo, María Cecilia Gamboa, Matías Rothhammer, Erwin Krauskopf

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Environmental stress factors such as salt, drought and heat are known to affect plant productivity. However, high salinity is spreading throughout the world, currently affecting more than 45 millionha. One of the mechanisms that allow plants to withstand salt stress consists on vacuolar sequestration of Na+, through a Na+/H+ antiporter. We isolated a new vacuolar Na+/H+ antiporter from Eucalyptus globulus from a cDNA library. The cDNA had a 1626bp open reading frame encoding a predicted protein of 542 amino acids with a deduced molecular weight of 59.1KDa. Phylogenetic and bioinformatic analyses indicated that EgNHX1 localized in the vacuole. To assess its role in Na+ exchange, we performed complementation studies using the Na+ sensitive yeast mutant strain Δnhx1. The results showed that EgNHX1 partially restored the salt sensitive phenotype of the yeast Δnhx1 strain. However, its overexpression in transgenic Arabidopsis confers tolerance in the presence of increasing NaCl concentrations while the wild type plants exhibited growth retardation. Expression profiles of Eucalyptus seedlings subjected to salt, drought, heat and ABA treatment were established. The results revealed that Egnhx1 was induced significantly only by drought. Together, these results suggest that the product of Egnhx1 from E. globulus is a functional vacuolar Na+/H+ antiporter.

Idioma originalEnglish
Páginas (desde-hasta)535-540
Número de páginas6
PublicaciónBiochemical and Biophysical Research Communications
Volumen430
N.º2
DOI
EstadoPublished - 11 ene 2013

Huella dactilar

Eucalyptus
Sodium-Hydrogen Antiporter
Drought
Droughts
Complementary DNA
Salts
Yeast
Hot Temperature
Yeasts
Salinity
Bioinformatics
Vacuoles
Computational Biology
Gene Library
Seedlings
Arabidopsis
Open Reading Frames
Molecular Weight
Productivity
Molecular weight

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Baltierra, Fabiola ; Castillo, Mabel ; Gamboa, María Cecilia ; Rothhammer, Matías ; Krauskopf, Erwin. / Molecular characterization of a novel Na+/H+ antiporter cDNA from Eucalyptus globulus. En: Biochemical and Biophysical Research Communications. 2013 ; Vol. 430, N.º 2. pp. 535-540.
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abstract = "Environmental stress factors such as salt, drought and heat are known to affect plant productivity. However, high salinity is spreading throughout the world, currently affecting more than 45 millionha. One of the mechanisms that allow plants to withstand salt stress consists on vacuolar sequestration of Na+, through a Na+/H+ antiporter. We isolated a new vacuolar Na+/H+ antiporter from Eucalyptus globulus from a cDNA library. The cDNA had a 1626bp open reading frame encoding a predicted protein of 542 amino acids with a deduced molecular weight of 59.1KDa. Phylogenetic and bioinformatic analyses indicated that EgNHX1 localized in the vacuole. To assess its role in Na+ exchange, we performed complementation studies using the Na+ sensitive yeast mutant strain Δnhx1. The results showed that EgNHX1 partially restored the salt sensitive phenotype of the yeast Δnhx1 strain. However, its overexpression in transgenic Arabidopsis confers tolerance in the presence of increasing NaCl concentrations while the wild type plants exhibited growth retardation. Expression profiles of Eucalyptus seedlings subjected to salt, drought, heat and ABA treatment were established. The results revealed that Egnhx1 was induced significantly only by drought. Together, these results suggest that the product of Egnhx1 from E. globulus is a functional vacuolar Na+/H+ antiporter.",
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Molecular characterization of a novel Na+/H+ antiporter cDNA from Eucalyptus globulus. / Baltierra, Fabiola; Castillo, Mabel; Gamboa, María Cecilia; Rothhammer, Matías; Krauskopf, Erwin.

En: Biochemical and Biophysical Research Communications, Vol. 430, N.º 2, 11.01.2013, p. 535-540.

Resultado de la investigación: Article

TY - JOUR

T1 - Molecular characterization of a novel Na+/H+ antiporter cDNA from Eucalyptus globulus

AU - Baltierra, Fabiola

AU - Castillo, Mabel

AU - Gamboa, María Cecilia

AU - Rothhammer, Matías

AU - Krauskopf, Erwin

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