Modulation of the activity of the transverse tubule Mg2+ATPase from frog skeletal muscle by a monoclonal antibody in vitro

Mario S. Rosemblatt, Gonzalo Pérez, Enrique Jaimovich

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

We have established several hybridoma lines that produce monoclonal antibodies against transverse tubule (t-tubule) proteins from frog skeletal muscle. The specificity of these antibodies was characterized by ELISA and Western immunoblotting with purified t-tubule, sarcoplasmic reticulum and partially purified sarcolemmal membranes. One of the monoclonal antibodies (2/34.4) recognizes a band of 109 000 Da in immunoblots. When purified frog t-tubule vesicles were preincubated with this antibody we observed an increase in the rate of the Mg2+-ATPase enzyme (up to six fold) which was dependent on antibody concentration. Immunocytological experiments done on cryostat sections of frog muscle indicate that the antigen recognized by this antibody is localized mainly at the level of the t-tubules (I band) and to a lesser extent at the sarcolemma. These results indicate that monoclonal antibody 2/34.4 recognizes the t-tubule Mg2+-ATPase and modulates its activity. This antibody should be useful as a probe on studies designed to study the physiological function of the enzyme.

Idioma originalInglés
Páginas (desde-hasta)99-107
Número de páginas9
PublicaciónMolecular and Cellular Biochemistry
Volumen106
N.º2
DOI
EstadoPublicada - ago 1991

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Genética
  • Bioquímica clínica
  • Biología celular
  • Medicina (todo)

Huella

Profundice en los temas de investigación de 'Modulation of the activity of the transverse tubule Mg<sup>2+</sup>ATPase from frog skeletal muscle by a monoclonal antibody in vitro'. En conjunto forman una huella única.

Citar esto