TY - JOUR
T1 - Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis
AU - Salinas-Grenet, Hernán
AU - Herrera-Vásquez, Ariel
AU - Parra, Samuel
AU - Cortez, Allan
AU - Gutiérrez, Lilian
AU - Pollmann, Stephan
AU - León, Gabriel
AU - Blanco-Herrera, Francisca
N1 - Funding Information:
Funding: This work was supported by the Fondo Nacional de Desarrollo Científico y Tecnológico (1170259) and the MIISSB Iniciativa Científica Milenio-MINECON to Francisca Blanco-Herrera, the Fondo Nacional de Desarrollo Científico y Tecnológico (1120766) to Gabriel León, and the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) (BFU2014-55575-R, BFU2017-82826-R) to Stephan Pollmann.
Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.
AB - Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.
KW - Anther
KW - Auxin
KW - Dehiscence
KW - IaaL
KW - Pollen
UR - http://www.scopus.com/inward/record.url?scp=85052539488&partnerID=8YFLogxK
U2 - 10.3390/ijms19082480
DO - 10.3390/ijms19082480
M3 - Article
AN - SCOPUS:85052539488
SN - 1661-6596
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
M1 - 2480
ER -