Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis

Hernán Salinas-Grenet, Ariel Herrera-Vásquez, Samuel Parra, Allan Cortez, Lilian Gutiérrez, Stephan Pollmann, Gabriel León, Francisca Blanco-Herrera

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.

Idioma originalEnglish
Número de artículo2480
PublicaciónInternational Journal of Molecular Sciences
Volumen19
N.º9
DOI
EstadoPublished - 1 sep 2018

Huella dactilar

auxins
pollen
Indoleacetic Acids
Pollen
Arabidopsis
Acetic acid
lysine
indoles
Modulation
modulation
Lysine
Ligases
Genes
acetic acid
genes
Seed
Elongation
Synchronization
Tissue
Pollen Tube

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Citar esto

Salinas-Grenet, Hernán ; Herrera-Vásquez, Ariel ; Parra, Samuel ; Cortez, Allan ; Gutiérrez, Lilian ; Pollmann, Stephan ; León, Gabriel ; Blanco-Herrera, Francisca. / Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis. En: International Journal of Molecular Sciences. 2018 ; Vol. 19, N.º 9.
@article{b7af235d5cb14c06ba0ac2e136be4687,
title = "Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis",
abstract = "Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.",
keywords = "Anther, Auxin, Dehiscence, IaaL, Pollen",
author = "Hern{\'a}n Salinas-Grenet and Ariel Herrera-V{\'a}squez and Samuel Parra and Allan Cortez and Lilian Guti{\'e}rrez and Stephan Pollmann and Gabriel Le{\'o}n and Francisca Blanco-Herrera",
year = "2018",
month = "9",
day = "1",
doi = "10.3390/ijms19082480",
language = "English",
volume = "19",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis. / Salinas-Grenet, Hernán; Herrera-Vásquez, Ariel; Parra, Samuel; Cortez, Allan; Gutiérrez, Lilian; Pollmann, Stephan; León, Gabriel; Blanco-Herrera, Francisca.

En: International Journal of Molecular Sciences, Vol. 19, N.º 9, 2480, 01.09.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - Modulation of auxin levels in pollen grains affects stamen development and anther dehiscence in Arabidopsis

AU - Salinas-Grenet, Hernán

AU - Herrera-Vásquez, Ariel

AU - Parra, Samuel

AU - Cortez, Allan

AU - Gutiérrez, Lilian

AU - Pollmann, Stephan

AU - León, Gabriel

AU - Blanco-Herrera, Francisca

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.

AB - Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.

KW - Anther

KW - Auxin

KW - Dehiscence

KW - IaaL

KW - Pollen

UR - http://www.scopus.com/inward/record.url?scp=85052539488&partnerID=8YFLogxK

U2 - 10.3390/ijms19082480

DO - 10.3390/ijms19082480

M3 - Article

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 9

M1 - 2480

ER -