Modulación opioide de la actividad antinociceptiva de L-arginina en ratones

L. Chanqueo, L. Yates, H. F. Miranda, G. Pinardi

Resultado de la investigación: Contribución a una revistaArtículo


Objectives: Nitric oxide (NO) is an intercellular neuromodulator synthetized by NO synthetase (NOS). L-arginine (L-ARG) is converted to ornithine and NO by NOS and is involved in the L-ARG-NO pathway of nociceptive transmission and/or modulation in the CNS and in the periphery. In the present work, the antinociceptive activity of L-ARG was determined and the effects of non selective and selective opioid antagonists was evaluated. Material and methods: The dose-response curve for the antinociceptive effect of intraperitoneal (i.p.) L-ARG was determined in CF-1 mice, using the acetic acid writhing test. The dose-response curves were repeated after pretreatment with the following opioid receptor antagonists: Naloxone and naltrexone (non selective), naltrindole (δ antagonist) y norbinaltorphimine (κ antagonist). The antinociceptive activity of a single dose of L-ARG administered intrathecally (i.t.) and intracerebro-ventricularly (i.c.v.) was determined by the same algesiometric test. Results: L-ARG i.p. exhibited a dose-dependent antinociceptive activity, with an ED 50 of 4,65 mg,kg -5 NOS inhibition significatively reduced the antinociceptive effect of L-ARG. The administration by the i.t. and i.c.v, routes of a dose approximately 45 times lower (0,16 and 0,15 -1) produced a comparable antinociceptive effect. The pretreatment with all opioid receptor antagonists significantly displaced the dose-response curve for the antinociceptive activity of L-ARG to the right, indicating a pharmacologic antagonism. Conclusions: Systemically administered L-ARG, through NO formation and activation of cyclic GMP induce antinociception probably by a direct effect on peripheral nociceptors, additionally activating spinal and supraspinal antinociceptive mechanisms. The relation between systemic, intrathecal and intracerebroventricular equipotent doses suggests that a major part of the acción is exerted in the CNS. The synthesis and release of NO facilitates in some way the activation of opioid receptors at spinal and supraspinal levels and confirms the modulating action of NO in nociception.

Título traducido de la contribuciónOpioid modulation of the antinociceptive activity of L-arginine in mice
Idioma originalEspañol
Páginas (desde-hasta)520-525
Número de páginas6
PublicaciónRevista de la Sociedad Espanola del Dolor
EstadoPublicada - 2000

Palabras clave

  • Antinociception
  • L-arginine
  • Nitric oxide
  • Opioid antagonists
  • Writhing test

Áreas temáticas de ASJC Scopus

  • Anestesiología y analgésicos

Huella Profundice en los temas de investigación de 'Modulación opioide de la actividad antinociceptiva de L-arginina en ratones'. En conjunto forman una huella única.

  • Citar esto

    Chanqueo, L., Yates, L., Miranda, H. F., & Pinardi, G. (2000). Modulación opioide de la actividad antinociceptiva de L-arginina en ratones. Revista de la Sociedad Espanola del Dolor, 7(8), 520-525.