Mini-Mu technology in Salmonella typhi: Isolation of stable MudJ operon fusions by cis complementation

I. Contreras, V. Obreque, B. Tesser, G. C. Mora

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

This paper describes a method to achieve stable MudJ insertions in the Salmonella typhi Ty2 chromosome. The method is a modification of the genetic complementation system described previously for Salmonella typhimurium, which consists in placing the defective transposon (MudJ) near the transposase genes of a helper Mu phage on a single DNA fragment. This fragment is then introduced into a new bacterial host by means of P22 transduction. We constructed a S. typhi strain which carries MudJ and the Mu helper phage in the chromosome. This strain was induced to lytic growth and the lysate was used to infect S. typhi Ty2. The frequency of mutation was 2.0 x 10-6 mutants per recipient bacterium. Superinfection with the Mu helper phage was about 1%. To determine the number of MudJ insertions, several mutants were subjected to Southern blot analysis. From a total of 25 mutants analyzed, only 4 contained more than one insertion. Our procedure compares well with the method described previously for the S. typhimurium-P22 system and can be applied to other Mu sensitive bacteria.

Idioma originalInglés
Páginas (desde-hasta)233-239
Número de páginas7
PublicaciónBiological Research
Volumen27
N.º3-4
EstadoPublicada - 1994

Áreas temáticas de ASJC Scopus

  • Agricultura y biología (todo)
  • Bioquímica, genética y biología molecular (todo)
  • Medicina (todo)

Huella

Profundice en los temas de investigación de 'Mini-Mu technology in Salmonella typhi: Isolation of stable MudJ operon fusions by cis complementation'. En conjunto forman una huella única.

Citar esto