Microbiological characterisation of the colonisation by Candida sp in patients with orthodontic fixed appliances and evaluation of host responses in saliva

Cecilia V. Tapia, Christian Batarce, José Amaro, German Hermosilla, Paula I. Rodas, Fabien Magne

Resultado de la investigación: Article

Resumen

Objectives: We investigated the colonisation by Candida spp in patients using orthodontic fixed appliances by characterising the isolated Candida strains and by evaluating the host oral mucosa response through the measure of human β-defensins 3 (HBD-3) expression and Interleukin-1ß/IL-10. Methods: Ninety patients were enrolled after signing an informed consent. Prevalence, susceptibility to fluconazole, genotyping and oral fungal burden of Candida sp. isolated were determined. Host responses were evaluated by measuring HBD-3 expression as well as IL-1ß and IL-10 in saliva. Results: The colonisation rate reached 6.7% (6/90), and 5 patients were colonised with C. albicans strains and one with one with C. tropicalis. The fluconazole MIC90/susceptibility of C. albicans strains ranged 1/0.25-1 μg/mL. However, isolated strains did not present different genotype (SAB>0.9), C. albicans colonisation seems to be influenced by the duration of treatment and by level expression of HBD3 that were higher in colonised patients (not statistically different). A negative correlation between the fungal burden and IL-1ß levels was found in colonised patients but not for IL-10. Conclusions: Our study revealed that patients with orthodontic fixed appliances were mainly colonised by C. albicans, which was related to a decrease in HBD-3 expression and IL-1ß levels.

Idioma originalEnglish
Páginas (desde-hasta)247-251
PublicaciónMycoses
Volumen62
N.º3
DOI
EstadoPublished - mar 2019

Huella dactilar

Orthodontic Appliances
Candida
Saliva
Interleukin-1
Interleukin-10
Fluconazole
Defensins
Mouth Mucosa
Informed Consent
Genotype

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Citar esto

Tapia, Cecilia V. ; Batarce, Christian ; Amaro, José ; Hermosilla, German ; Rodas, Paula I. ; Magne, Fabien. / Microbiological characterisation of the colonisation by Candida sp in patients with orthodontic fixed appliances and evaluation of host responses in saliva. En: Mycoses. 2019 ; Vol. 62, N.º 3. pp. 247-251.
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abstract = "Objectives: We investigated the colonisation by Candida spp in patients using orthodontic fixed appliances by characterising the isolated Candida strains and by evaluating the host oral mucosa response through the measure of human β-defensins 3 (HBD-3) expression and Interleukin-1{\ss}/IL-10. Methods: Ninety patients were enrolled after signing an informed consent. Prevalence, susceptibility to fluconazole, genotyping and oral fungal burden of Candida sp. isolated were determined. Host responses were evaluated by measuring HBD-3 expression as well as IL-1{\ss} and IL-10 in saliva. Results: The colonisation rate reached 6.7{\%} (6/90), and 5 patients were colonised with C. albicans strains and one with one with C. tropicalis. The fluconazole MIC90/susceptibility of C. albicans strains ranged 1/0.25-1 μg/mL. However, isolated strains did not present different genotype (SAB>0.9), C. albicans colonisation seems to be influenced by the duration of treatment and by level expression of HBD3 that were higher in colonised patients (not statistically different). A negative correlation between the fungal burden and IL-1{\ss} levels was found in colonised patients but not for IL-10. Conclusions: Our study revealed that patients with orthodontic fixed appliances were mainly colonised by C. albicans, which was related to a decrease in HBD-3 expression and IL-1{\ss} levels.",
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Microbiological characterisation of the colonisation by Candida sp in patients with orthodontic fixed appliances and evaluation of host responses in saliva. / Tapia, Cecilia V.; Batarce, Christian; Amaro, José; Hermosilla, German; Rodas, Paula I.; Magne, Fabien.

En: Mycoses, Vol. 62, N.º 3, 03.2019, p. 247-251.

Resultado de la investigación: Article

TY - JOUR

T1 - Microbiological characterisation of the colonisation by Candida sp in patients with orthodontic fixed appliances and evaluation of host responses in saliva

AU - Tapia, Cecilia V.

AU - Batarce, Christian

AU - Amaro, José

AU - Hermosilla, German

AU - Rodas, Paula I.

AU - Magne, Fabien

PY - 2019/3

Y1 - 2019/3

N2 - Objectives: We investigated the colonisation by Candida spp in patients using orthodontic fixed appliances by characterising the isolated Candida strains and by evaluating the host oral mucosa response through the measure of human β-defensins 3 (HBD-3) expression and Interleukin-1ß/IL-10. Methods: Ninety patients were enrolled after signing an informed consent. Prevalence, susceptibility to fluconazole, genotyping and oral fungal burden of Candida sp. isolated were determined. Host responses were evaluated by measuring HBD-3 expression as well as IL-1ß and IL-10 in saliva. Results: The colonisation rate reached 6.7% (6/90), and 5 patients were colonised with C. albicans strains and one with one with C. tropicalis. The fluconazole MIC90/susceptibility of C. albicans strains ranged 1/0.25-1 μg/mL. However, isolated strains did not present different genotype (SAB>0.9), C. albicans colonisation seems to be influenced by the duration of treatment and by level expression of HBD3 that were higher in colonised patients (not statistically different). A negative correlation between the fungal burden and IL-1ß levels was found in colonised patients but not for IL-10. Conclusions: Our study revealed that patients with orthodontic fixed appliances were mainly colonised by C. albicans, which was related to a decrease in HBD-3 expression and IL-1ß levels.

AB - Objectives: We investigated the colonisation by Candida spp in patients using orthodontic fixed appliances by characterising the isolated Candida strains and by evaluating the host oral mucosa response through the measure of human β-defensins 3 (HBD-3) expression and Interleukin-1ß/IL-10. Methods: Ninety patients were enrolled after signing an informed consent. Prevalence, susceptibility to fluconazole, genotyping and oral fungal burden of Candida sp. isolated were determined. Host responses were evaluated by measuring HBD-3 expression as well as IL-1ß and IL-10 in saliva. Results: The colonisation rate reached 6.7% (6/90), and 5 patients were colonised with C. albicans strains and one with one with C. tropicalis. The fluconazole MIC90/susceptibility of C. albicans strains ranged 1/0.25-1 μg/mL. However, isolated strains did not present different genotype (SAB>0.9), C. albicans colonisation seems to be influenced by the duration of treatment and by level expression of HBD3 that were higher in colonised patients (not statistically different). A negative correlation between the fungal burden and IL-1ß levels was found in colonised patients but not for IL-10. Conclusions: Our study revealed that patients with orthodontic fixed appliances were mainly colonised by C. albicans, which was related to a decrease in HBD-3 expression and IL-1ß levels.

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KW - Candida albicans

KW - HBD-3

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JO - Mycoses

JF - Mycoses

SN - 0933-7407

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