TY - JOUR
T1 - Mg-Al LDH nanosheets as a nanotechnological tool in agriculture
T2 - an exploratory toxicity evaluation study
AU - Salinas-Jiménez, Rafael
AU - Vera, Gloria
AU - Tobar, Mariola
AU - Moscote, Jeimy
AU - Acha, Giovana
AU - Herrera-Vásquez, Ariel
AU - Rojas-Rivera, Diego
AU - Vidal, Elena A.
AU - Almeida, Andrea Miyasaka
AU - Ahumada, Manuel
N1 - Publisher Copyright:
© 2024 The Royal Society of Chemistry.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - In this work, we studied the impact of a Mg-Al layered double hydroxide (LDH) nanosheet formulation—nanoparticles recently recommended for the industry owing to their use as a carrier for the controlled delivery of agrochemicals. The prepared Mg-Al LDH had a lateral size close to 35 nm, a positively charged surface (+34.6 mV), and a flake-like shape. Its potential toxicity in plants was evaluated in protoplasts from Arabidopsis thaliana and on the leaves of A. thaliana and Solanum lycopersicum using leaf infiltration assays. Furthermore, the toxicity of the nanoparticles was evaluated in Botrytis cinerea and Trichoderma atroviride, two fungal organisms commonly present in agricultural contexts. The results indicate that the Mg-Al LDH is not toxic for protoplasts within the tested range (0-1000 ng μL−1), with some cell death associated with an osmotic/ionic strength effect. Moreover, leaf infiltration assays did not show damage after 48 h treatment. Finally, the nanoparticles did not affect the growth rate of the pathogenic fungus Botrytis cinerea up to 750 ng μL−1, while the plant growth-promoting and bio-controller fungi Trichoderma atroviride showed an increase in its growth rate at all the tested concentrations, particularly over 250 ng μL−1. Together, these results indicate that the concentrations of the Mg-Al LDH employed in this work are not toxic and could be utilized in agriculture as a carrier for agrochemicals. Further studies should be performed to unravel any potential toxicity of nanoparticles, and this research strongly contributes to uncovering any potential toxicity of Mg-Al LDH for agriculture.
AB - In this work, we studied the impact of a Mg-Al layered double hydroxide (LDH) nanosheet formulation—nanoparticles recently recommended for the industry owing to their use as a carrier for the controlled delivery of agrochemicals. The prepared Mg-Al LDH had a lateral size close to 35 nm, a positively charged surface (+34.6 mV), and a flake-like shape. Its potential toxicity in plants was evaluated in protoplasts from Arabidopsis thaliana and on the leaves of A. thaliana and Solanum lycopersicum using leaf infiltration assays. Furthermore, the toxicity of the nanoparticles was evaluated in Botrytis cinerea and Trichoderma atroviride, two fungal organisms commonly present in agricultural contexts. The results indicate that the Mg-Al LDH is not toxic for protoplasts within the tested range (0-1000 ng μL−1), with some cell death associated with an osmotic/ionic strength effect. Moreover, leaf infiltration assays did not show damage after 48 h treatment. Finally, the nanoparticles did not affect the growth rate of the pathogenic fungus Botrytis cinerea up to 750 ng μL−1, while the plant growth-promoting and bio-controller fungi Trichoderma atroviride showed an increase in its growth rate at all the tested concentrations, particularly over 250 ng μL−1. Together, these results indicate that the concentrations of the Mg-Al LDH employed in this work are not toxic and could be utilized in agriculture as a carrier for agrochemicals. Further studies should be performed to unravel any potential toxicity of nanoparticles, and this research strongly contributes to uncovering any potential toxicity of Mg-Al LDH for agriculture.
UR - http://www.scopus.com/inward/record.url?scp=85190747819&partnerID=8YFLogxK
U2 - 10.1039/d3en00733b
DO - 10.1039/d3en00733b
M3 - Article
AN - SCOPUS:85190747819
SN - 2051-8153
VL - 11
SP - 2249
EP - 2261
JO - Environmental Science: Nano
JF - Environmental Science: Nano
IS - 5
ER -