Metabolomic Analysis of Two Parmotrema Lichens: P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS

Alfredo Torres-Benítez, Mariá Rivera-Montalvo, Beatriz Sepúlveda, Olivio N. Castro, Edgar Nagles, Mario J. Simirgiotis, Olimpo Garciá-Beltrán, Carlos Areche

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria. Lichens belonging to the Parmeliaceae family comprise 2700 species of lichens, including the Parmotrema genus which is composed of 300 species. The metabolites of this genus include depsides, depsidones, phenolics, polysaccharides, lipids, diphenylethers and dibenzofurans, which are responsible for the biological activities reported including antidiabetic, antihelmintic, anticancer, antioxidant, antibacterial, anti-inflammatory, antimitotic, antitumoral, antifungal, and antioxidant enzyme inhibitory. Due to scarce knowledge of metabolomic profiles of Parmotrema species (P. andinum and P. robustum), a full metabolome study based on ultra-high performance liquid chromatography-diode array detector-electrospray ionization-quadrupole-orbitrap-mass-spectrometry (UHPLC-DAD-ESI-Q-orbitrap MS) was performed for a comprehensive characterization of their substances. From the methanolic extracts of these species, a total of 54 metabolites were identified for the first time using this hyphenated technique, including thirty compounds in P. andinum, and thirty-seven in P. robustum. Moreover, two compounds were not identified as known compounds, and could be new structures, according to our data. This report shows that this technique is effective and accurate for rapid chemical identification of lichen substances and the compounds identified could serve as chemotaxonomic markers to differentiate these ruffle lichens.

Idioma originalEnglish
Número de artículo1861
PublicaciónMolecules
Volumen22
N.º11
DOI
EstadoPublished - 1 nov 2017
Publicado de forma externa

Huella dactilar

lichens
Lichens
Metabolomics
metabolites
antioxidants
Metabolites
Depsides
Antioxidants
Antimitotic Agents
Microalgae
Electrospray ionization
Anthelmintics
Metabolome
fungi
polysaccharides
liquid chromatography
High performance liquid chromatography
Cyanobacteria
activity (biology)
Bioactivity

ASJC Scopus subject areas

  • Organic Chemistry

Citar esto

Torres-Benítez, A., Rivera-Montalvo, M., Sepúlveda, B., Castro, O. N., Nagles, E., Simirgiotis, M. J., ... Areche, C. (2017). Metabolomic Analysis of Two Parmotrema Lichens: P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS. Molecules, 22(11), [1861]. https://doi.org/10.3390/molecules22111861
Torres-Benítez, Alfredo ; Rivera-Montalvo, Mariá ; Sepúlveda, Beatriz ; Castro, Olivio N. ; Nagles, Edgar ; Simirgiotis, Mario J. ; Garciá-Beltrán, Olimpo ; Areche, Carlos. / Metabolomic Analysis of Two Parmotrema Lichens : P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS. En: Molecules. 2017 ; Vol. 22, N.º 11.
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abstract = "Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria. Lichens belonging to the Parmeliaceae family comprise 2700 species of lichens, including the Parmotrema genus which is composed of 300 species. The metabolites of this genus include depsides, depsidones, phenolics, polysaccharides, lipids, diphenylethers and dibenzofurans, which are responsible for the biological activities reported including antidiabetic, antihelmintic, anticancer, antioxidant, antibacterial, anti-inflammatory, antimitotic, antitumoral, antifungal, and antioxidant enzyme inhibitory. Due to scarce knowledge of metabolomic profiles of Parmotrema species (P. andinum and P. robustum), a full metabolome study based on ultra-high performance liquid chromatography-diode array detector-electrospray ionization-quadrupole-orbitrap-mass-spectrometry (UHPLC-DAD-ESI-Q-orbitrap MS) was performed for a comprehensive characterization of their substances. From the methanolic extracts of these species, a total of 54 metabolites were identified for the first time using this hyphenated technique, including thirty compounds in P. andinum, and thirty-seven in P. robustum. Moreover, two compounds were not identified as known compounds, and could be new structures, according to our data. This report shows that this technique is effective and accurate for rapid chemical identification of lichen substances and the compounds identified could serve as chemotaxonomic markers to differentiate these ruffle lichens.",
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Torres-Benítez, A, Rivera-Montalvo, M, Sepúlveda, B, Castro, ON, Nagles, E, Simirgiotis, MJ, Garciá-Beltrán, O & Areche, C 2017, 'Metabolomic Analysis of Two Parmotrema Lichens: P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS', Molecules, vol. 22, n.º 11, 1861. https://doi.org/10.3390/molecules22111861

Metabolomic Analysis of Two Parmotrema Lichens : P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS. / Torres-Benítez, Alfredo; Rivera-Montalvo, Mariá; Sepúlveda, Beatriz; Castro, Olivio N.; Nagles, Edgar; Simirgiotis, Mario J.; Garciá-Beltrán, Olimpo; Areche, Carlos.

En: Molecules, Vol. 22, N.º 11, 1861, 01.11.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Metabolomic Analysis of Two Parmotrema Lichens

T2 - P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS

AU - Torres-Benítez, Alfredo

AU - Rivera-Montalvo, Mariá

AU - Sepúlveda, Beatriz

AU - Castro, Olivio N.

AU - Nagles, Edgar

AU - Simirgiotis, Mario J.

AU - Garciá-Beltrán, Olimpo

AU - Areche, Carlos

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria. Lichens belonging to the Parmeliaceae family comprise 2700 species of lichens, including the Parmotrema genus which is composed of 300 species. The metabolites of this genus include depsides, depsidones, phenolics, polysaccharides, lipids, diphenylethers and dibenzofurans, which are responsible for the biological activities reported including antidiabetic, antihelmintic, anticancer, antioxidant, antibacterial, anti-inflammatory, antimitotic, antitumoral, antifungal, and antioxidant enzyme inhibitory. Due to scarce knowledge of metabolomic profiles of Parmotrema species (P. andinum and P. robustum), a full metabolome study based on ultra-high performance liquid chromatography-diode array detector-electrospray ionization-quadrupole-orbitrap-mass-spectrometry (UHPLC-DAD-ESI-Q-orbitrap MS) was performed for a comprehensive characterization of their substances. From the methanolic extracts of these species, a total of 54 metabolites were identified for the first time using this hyphenated technique, including thirty compounds in P. andinum, and thirty-seven in P. robustum. Moreover, two compounds were not identified as known compounds, and could be new structures, according to our data. This report shows that this technique is effective and accurate for rapid chemical identification of lichen substances and the compounds identified could serve as chemotaxonomic markers to differentiate these ruffle lichens.

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KW - Metabolomic

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KW - UHPLC-MS-MS

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Torres-Benítez A, Rivera-Montalvo M, Sepúlveda B, Castro ON, Nagles E, Simirgiotis MJ y otros. Metabolomic Analysis of Two Parmotrema Lichens: P. robustum (Degel.) Hale and P. andinum (Mull. rg.) Hale Using UHPLC-ESI-OT-MS-MS. Molecules. 2017 nov 1;22(11). 1861. https://doi.org/10.3390/molecules22111861