Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model

Julia Tandberg, Cristian Oliver, Leidy Lagos, Mona Gaarder, Alejandro J. Yáñez, Erik Ropstad, Hanne C. Winther-Larsen

Resultado de la investigación: Article

6 Citas (Scopus)

Resumen

Infections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis.

Idioma originalEnglish
Páginas (desde-hasta)189-198
Número de páginas10
PublicaciónFish and Shellfish Immunology
Volumen67
DOI
EstadoPublished - 1 ago 2017

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
septicemia
salmonid
Zebrafish
immunity
vesicle
Danio rerio
Immunity
Sepsis
membrane
Membranes
vaccines
vaccine
Vaccines
antibiotics
Anti-Bacterial Agents
lethal dose
Aquaculture
membrane potential

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science
  • Medicine(all)

Citar esto

Tandberg, Julia ; Oliver, Cristian ; Lagos, Leidy ; Gaarder, Mona ; Yáñez, Alejandro J. ; Ropstad, Erik ; Winther-Larsen, Hanne C. / Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model. En: Fish and Shellfish Immunology. 2017 ; Vol. 67. pp. 189-198.
@article{245bd10e0721471287820c077bd33f15,
title = "Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model",
abstract = "Infections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis.",
keywords = "Immune response, MVs, Piscirickettsia salmonis, SRS, Vaccine, Zebrafish",
author = "Julia Tandberg and Cristian Oliver and Leidy Lagos and Mona Gaarder and Y{\'a}{\~n}ez, {Alejandro J.} and Erik Ropstad and Winther-Larsen, {Hanne C.}",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.fsi.2017.06.015",
language = "English",
volume = "67",
pages = "189--198",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Academic Press Inc.",

}

Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model. / Tandberg, Julia; Oliver, Cristian; Lagos, Leidy; Gaarder, Mona; Yáñez, Alejandro J.; Ropstad, Erik; Winther-Larsen, Hanne C.

En: Fish and Shellfish Immunology, Vol. 67, 01.08.2017, p. 189-198.

Resultado de la investigación: Article

TY - JOUR

T1 - Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model

AU - Tandberg, Julia

AU - Oliver, Cristian

AU - Lagos, Leidy

AU - Gaarder, Mona

AU - Yáñez, Alejandro J.

AU - Ropstad, Erik

AU - Winther-Larsen, Hanne C.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Infections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis.

AB - Infections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis.

KW - Immune response

KW - MVs

KW - Piscirickettsia salmonis

KW - SRS

KW - Vaccine

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85020734973&partnerID=8YFLogxK

U2 - 10.1016/j.fsi.2017.06.015

DO - 10.1016/j.fsi.2017.06.015

M3 - Article

AN - SCOPUS:85020734973

VL - 67

SP - 189

EP - 198

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -