Membrane-initiated cortisol action modulates early pyruvate dehydrogenase kinase 2 (pdk2) expression in fish skeletal muscle

Jorge E. Aedo, Rodrigo Zuloaga, Sebastián Boltaña, Alfredo Molina, Juan Antonio Valdés

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)


Cortisol is the main glucocorticoid hormone in teleosts involved in the regulation of metabolic adjustments under both normal and stressful physiological conditions. In the skeletal muscle, cortisol modulates the energetic metabolism promoting the mobilization of glucose and other energetic substrates to overcome the stress stimulus. The effects of cortisol-mediated stress response are attributed to canonical/genomic mechanisms which involve the interaction of the hormone with its intracellular glucocorticoid receptor and, consequently, modulation of target genes. However, cortisol also can interact with membrane components, activating rapid signaling pathways with unknown contribution during the early stress response. In the present work, we evaluated the impact of membrane-initiated cortisol action over the expression of the critical modulator of energetic metabolism, pyruvate dehydrogenase kinase 2 (pdk2), in fish skeletal muscle. Juvenile rainbow trout were intraperitoneally administered with stress-related doses of cortisol and cortisol-BSA, and the expression of pdk2 was assayed by using RT-qPCR. Our results reveal that pdk2 mRNA levels increased in the skeletal muscle at one hour in both cortisol- and cortisol-BSA-treated fish. Moreover, in vitro studies revealed a biphasic response over the pdk2 regulation in myotubes mediated first through membrane-cortisol signaling pathways followed by the classic cortisol action. Finally, pdk2 up-regulation owing to cortisol and cortisol-BSA is reverted in RU486 treated myotubes, suggesting that GR signaling participates in both cortisol signaling pathways. This work suggests that non-classical cortisol pathways contribute to regulate the early metabolic response to stress in fish skeletal muscle.

Idioma originalInglés
Páginas (desde-hasta)24-29
Número de páginas6
PublicaciónComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
EstadoPublicada - 1 jul. 2019

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Fisiología
  • Biología molecular


Profundice en los temas de investigación de 'Membrane-initiated cortisol action modulates early pyruvate dehydrogenase kinase 2 (pdk2) expression in fish skeletal muscle'. En conjunto forman una huella única.

Citar esto