Maximal oxygen consumption in relation to subordinate traits in lines of house mice selectively bred for high voluntary wheel running

Enrico L. Rezende, Fernando R. Gomes, Jessica L. Malisch, Mark A. Chappell, Theodore Garland

Resultado de la investigación: Article

61 Citas (Scopus)

Resumen

We studied relations between maximal O2 consumption (V̇O2 max) during forced exercise and subordinate traits associated with blood O2 transport and cellular respiration in four lines of mice selectively bred for high voluntary wheel running (S lines) and their four nonselected control (C) lines. Previously, we reported V̇O 2 max of 59 females at three PO2 (hypoxia = 14% O 2, normoxia = 21%, hyperoxia = 30%). Here, we test the hypothesis that variation in V̇O2 max can be explained, in part, by hemoglobin concentration and PO2 necessary to obtain 50% O 2 saturation of Hb (an estimate of Hb affinity for O2) of the blood as well as citrate synthase activity and myoglobin concentration of ventricles and gastrocnemius muscle. Statistical analyses controlled for body mass, compared S and C lines, and also considered effects of the mini-muscle phenotype (present only in S lines and resulting from a Mendelian recessive allele), which reduces hindlimb muscle mass while increasing muscle mass-specific aerobic capacity. Although S lines had higher V̇O 2 max than C, subordinate traits showed no statistical differences when the presence of the mini-muscle phenotype was controlled. However, subordinate traits did account for some of the individual variation in V̇O2 max. Ventricle size was a positive predictor of V̇O 2 max at all three PO2. Blood Hb concentration was a positive predictor of V̇O2 max in S lines but a negative predictor in C lines, indicating that the physiological underpinnings of V̇O2 max have been altered by selective breeding. Mice with the mini-muscle phenotype had enlarged ventricles, with higher mass-specific citrate synthase activity and myoglobin concentration, which may account for their higher V̇O2 max in hypoxia.

Idioma originalEnglish
Páginas (desde-hasta)477-485
Número de páginas9
PublicaciónJournal of Applied Physiology
Volumen101
N.º2
DOI
EstadoPublished - 2006

Huella dactilar

Oxygen Consumption
Running
Muscles
Citrate (si)-Synthase
Myoglobin
Phenotype
Cell Respiration
Hyperoxia
Hindlimb
Hemoglobins
Skeletal Muscle
Alleles
Hypoxia

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Citar esto

Rezende, Enrico L. ; Gomes, Fernando R. ; Malisch, Jessica L. ; Chappell, Mark A. ; Garland, Theodore. / Maximal oxygen consumption in relation to subordinate traits in lines of house mice selectively bred for high voluntary wheel running. En: Journal of Applied Physiology. 2006 ; Vol. 101, N.º 2. pp. 477-485.
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title = "Maximal oxygen consumption in relation to subordinate traits in lines of house mice selectively bred for high voluntary wheel running",
abstract = "We studied relations between maximal O2 consumption (V̇O2 max) during forced exercise and subordinate traits associated with blood O2 transport and cellular respiration in four lines of mice selectively bred for high voluntary wheel running (S lines) and their four nonselected control (C) lines. Previously, we reported V̇O 2 max of 59 females at three PO2 (hypoxia = 14{\%} O 2, normoxia = 21{\%}, hyperoxia = 30{\%}). Here, we test the hypothesis that variation in V̇O2 max can be explained, in part, by hemoglobin concentration and PO2 necessary to obtain 50{\%} O 2 saturation of Hb (an estimate of Hb affinity for O2) of the blood as well as citrate synthase activity and myoglobin concentration of ventricles and gastrocnemius muscle. Statistical analyses controlled for body mass, compared S and C lines, and also considered effects of the mini-muscle phenotype (present only in S lines and resulting from a Mendelian recessive allele), which reduces hindlimb muscle mass while increasing muscle mass-specific aerobic capacity. Although S lines had higher V̇O 2 max than C, subordinate traits showed no statistical differences when the presence of the mini-muscle phenotype was controlled. However, subordinate traits did account for some of the individual variation in V̇O2 max. Ventricle size was a positive predictor of V̇O 2 max at all three PO2. Blood Hb concentration was a positive predictor of V̇O2 max in S lines but a negative predictor in C lines, indicating that the physiological underpinnings of V̇O2 max have been altered by selective breeding. Mice with the mini-muscle phenotype had enlarged ventricles, with higher mass-specific citrate synthase activity and myoglobin concentration, which may account for their higher V̇O2 max in hypoxia.",
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Maximal oxygen consumption in relation to subordinate traits in lines of house mice selectively bred for high voluntary wheel running. / Rezende, Enrico L.; Gomes, Fernando R.; Malisch, Jessica L.; Chappell, Mark A.; Garland, Theodore.

En: Journal of Applied Physiology, Vol. 101, N.º 2, 2006, p. 477-485.

Resultado de la investigación: Article

TY - JOUR

T1 - Maximal oxygen consumption in relation to subordinate traits in lines of house mice selectively bred for high voluntary wheel running

AU - Rezende, Enrico L.

AU - Gomes, Fernando R.

AU - Malisch, Jessica L.

AU - Chappell, Mark A.

AU - Garland, Theodore

PY - 2006

Y1 - 2006

N2 - We studied relations between maximal O2 consumption (V̇O2 max) during forced exercise and subordinate traits associated with blood O2 transport and cellular respiration in four lines of mice selectively bred for high voluntary wheel running (S lines) and their four nonselected control (C) lines. Previously, we reported V̇O 2 max of 59 females at three PO2 (hypoxia = 14% O 2, normoxia = 21%, hyperoxia = 30%). Here, we test the hypothesis that variation in V̇O2 max can be explained, in part, by hemoglobin concentration and PO2 necessary to obtain 50% O 2 saturation of Hb (an estimate of Hb affinity for O2) of the blood as well as citrate synthase activity and myoglobin concentration of ventricles and gastrocnemius muscle. Statistical analyses controlled for body mass, compared S and C lines, and also considered effects of the mini-muscle phenotype (present only in S lines and resulting from a Mendelian recessive allele), which reduces hindlimb muscle mass while increasing muscle mass-specific aerobic capacity. Although S lines had higher V̇O 2 max than C, subordinate traits showed no statistical differences when the presence of the mini-muscle phenotype was controlled. However, subordinate traits did account for some of the individual variation in V̇O2 max. Ventricle size was a positive predictor of V̇O 2 max at all three PO2. Blood Hb concentration was a positive predictor of V̇O2 max in S lines but a negative predictor in C lines, indicating that the physiological underpinnings of V̇O2 max have been altered by selective breeding. Mice with the mini-muscle phenotype had enlarged ventricles, with higher mass-specific citrate synthase activity and myoglobin concentration, which may account for their higher V̇O2 max in hypoxia.

AB - We studied relations between maximal O2 consumption (V̇O2 max) during forced exercise and subordinate traits associated with blood O2 transport and cellular respiration in four lines of mice selectively bred for high voluntary wheel running (S lines) and their four nonselected control (C) lines. Previously, we reported V̇O 2 max of 59 females at three PO2 (hypoxia = 14% O 2, normoxia = 21%, hyperoxia = 30%). Here, we test the hypothesis that variation in V̇O2 max can be explained, in part, by hemoglobin concentration and PO2 necessary to obtain 50% O 2 saturation of Hb (an estimate of Hb affinity for O2) of the blood as well as citrate synthase activity and myoglobin concentration of ventricles and gastrocnemius muscle. Statistical analyses controlled for body mass, compared S and C lines, and also considered effects of the mini-muscle phenotype (present only in S lines and resulting from a Mendelian recessive allele), which reduces hindlimb muscle mass while increasing muscle mass-specific aerobic capacity. Although S lines had higher V̇O 2 max than C, subordinate traits showed no statistical differences when the presence of the mini-muscle phenotype was controlled. However, subordinate traits did account for some of the individual variation in V̇O2 max. Ventricle size was a positive predictor of V̇O 2 max at all three PO2. Blood Hb concentration was a positive predictor of V̇O2 max in S lines but a negative predictor in C lines, indicating that the physiological underpinnings of V̇O2 max have been altered by selective breeding. Mice with the mini-muscle phenotype had enlarged ventricles, with higher mass-specific citrate synthase activity and myoglobin concentration, which may account for their higher V̇O2 max in hypoxia.

KW - Cardiac output

KW - Experimental evolution

KW - Hemoglobin

KW - Hypoxia tolerance

KW - Myoglobin

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U2 - 10.1152/japplphysiol.00042.2006

DO - 10.1152/japplphysiol.00042.2006

M3 - Article

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VL - 101

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