Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress: Variation in Its Levels and Activity Distribution in Rat Hippocampus

Felipe I. Aguayo, Aníbal A. Pacheco, Gonzalo J. García-Rojo, Javier A. Pizarro-Bauerle, Ana V. Doberti, Macarena Tejos, María A. García-Pérez, Paulina S. Rojas, Jenny L. Fiedler

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

A single stress exposure facilitates memory formation through neuroplastic processes that reshape excitatory synapses in the hippocampus, probably requiring changes in extracellular matrix components. We tested the hypothesis that matrix metalloproteinase 9 (MMP-9), an enzyme that degrades components of extracellular matrix and synaptic proteins such as β-dystroglycan (β-DG43), changes their activity and distribution in rat hippocampus during the acute stress response. After 2.5 h of restraint stress, we found (i) increased MMP-9 levels and potential activity in whole hippocampal extracts, accompanied by β-DG43 cleavage, and (ii) a significant enhancement of MMP-9 immunoreactivity in dendritic fields such as stratum radiatum and the molecular layer of hippocampus. After 24 h of stress, we found that (i) MMP-9 net activity rises at somatic field, i.e., stratum pyramidale and granule cell layers, and also at synaptic field, mainly stratum radiatum and the molecular layer of hippocampus, and (ii) hippocampal synaptoneurosome fractions are enriched with MMP-9, without variation of its potential enzymatic activity, in accordance with the constant level of cleaved β-DG43. These findings indicate that stress triggers a peculiar timing response in the MMP-9 levels, net activity, and subcellular distribution in the hippocampus, suggesting its involvement in the processing of substrates during the stress response.

Idioma originalEnglish
Páginas (desde-hasta)945-956
Número de páginas12
PublicaciónACS Chemical Neuroscience
Volumen9
N.º5
DOI
EstadoPublished - 16 may 2018

Huella dactilar

Matrix Metalloproteinase 9
Rats
Hippocampus
Display devices
Dystroglycans
Extracellular Matrix Proteins
Synapses
Extracellular Matrix
Cells
Data storage equipment
Substrates
Enzymes
Processing
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

Citar esto

Aguayo, F. I., Pacheco, A. A., García-Rojo, G. J., Pizarro-Bauerle, J. A., Doberti, A. V., Tejos, M., ... Fiedler, J. L. (2018). Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress: Variation in Its Levels and Activity Distribution in Rat Hippocampus. ACS Chemical Neuroscience, 9(5), 945-956. https://doi.org/10.1021/acschemneuro.7b00387
Aguayo, Felipe I. ; Pacheco, Aníbal A. ; García-Rojo, Gonzalo J. ; Pizarro-Bauerle, Javier A. ; Doberti, Ana V. ; Tejos, Macarena ; García-Pérez, María A. ; Rojas, Paulina S. ; Fiedler, Jenny L. / Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress : Variation in Its Levels and Activity Distribution in Rat Hippocampus. En: ACS Chemical Neuroscience. 2018 ; Vol. 9, N.º 5. pp. 945-956.
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abstract = "A single stress exposure facilitates memory formation through neuroplastic processes that reshape excitatory synapses in the hippocampus, probably requiring changes in extracellular matrix components. We tested the hypothesis that matrix metalloproteinase 9 (MMP-9), an enzyme that degrades components of extracellular matrix and synaptic proteins such as β-dystroglycan (β-DG43), changes their activity and distribution in rat hippocampus during the acute stress response. After 2.5 h of restraint stress, we found (i) increased MMP-9 levels and potential activity in whole hippocampal extracts, accompanied by β-DG43 cleavage, and (ii) a significant enhancement of MMP-9 immunoreactivity in dendritic fields such as stratum radiatum and the molecular layer of hippocampus. After 24 h of stress, we found that (i) MMP-9 net activity rises at somatic field, i.e., stratum pyramidale and granule cell layers, and also at synaptic field, mainly stratum radiatum and the molecular layer of hippocampus, and (ii) hippocampal synaptoneurosome fractions are enriched with MMP-9, without variation of its potential enzymatic activity, in accordance with the constant level of cleaved β-DG43. These findings indicate that stress triggers a peculiar timing response in the MMP-9 levels, net activity, and subcellular distribution in the hippocampus, suggesting its involvement in the processing of substrates during the stress response.",
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Aguayo, FI, Pacheco, AA, García-Rojo, GJ, Pizarro-Bauerle, JA, Doberti, AV, Tejos, M, García-Pérez, MA, Rojas, PS & Fiedler, JL 2018, 'Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress: Variation in Its Levels and Activity Distribution in Rat Hippocampus', ACS Chemical Neuroscience, vol. 9, n.º 5, pp. 945-956. https://doi.org/10.1021/acschemneuro.7b00387

Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress : Variation in Its Levels and Activity Distribution in Rat Hippocampus. / Aguayo, Felipe I.; Pacheco, Aníbal A.; García-Rojo, Gonzalo J.; Pizarro-Bauerle, Javier A.; Doberti, Ana V.; Tejos, Macarena; García-Pérez, María A.; Rojas, Paulina S.; Fiedler, Jenny L.

En: ACS Chemical Neuroscience, Vol. 9, N.º 5, 16.05.2018, p. 945-956.

Resultado de la investigación: Article

TY - JOUR

T1 - Matrix Metalloproteinase 9 Displays a Particular Time Response to Acute Stress

T2 - Variation in Its Levels and Activity Distribution in Rat Hippocampus

AU - Aguayo, Felipe I.

AU - Pacheco, Aníbal A.

AU - García-Rojo, Gonzalo J.

AU - Pizarro-Bauerle, Javier A.

AU - Doberti, Ana V.

AU - Tejos, Macarena

AU - García-Pérez, María A.

AU - Rojas, Paulina S.

AU - Fiedler, Jenny L.

PY - 2018/5/16

Y1 - 2018/5/16

N2 - A single stress exposure facilitates memory formation through neuroplastic processes that reshape excitatory synapses in the hippocampus, probably requiring changes in extracellular matrix components. We tested the hypothesis that matrix metalloproteinase 9 (MMP-9), an enzyme that degrades components of extracellular matrix and synaptic proteins such as β-dystroglycan (β-DG43), changes their activity and distribution in rat hippocampus during the acute stress response. After 2.5 h of restraint stress, we found (i) increased MMP-9 levels and potential activity in whole hippocampal extracts, accompanied by β-DG43 cleavage, and (ii) a significant enhancement of MMP-9 immunoreactivity in dendritic fields such as stratum radiatum and the molecular layer of hippocampus. After 24 h of stress, we found that (i) MMP-9 net activity rises at somatic field, i.e., stratum pyramidale and granule cell layers, and also at synaptic field, mainly stratum radiatum and the molecular layer of hippocampus, and (ii) hippocampal synaptoneurosome fractions are enriched with MMP-9, without variation of its potential enzymatic activity, in accordance with the constant level of cleaved β-DG43. These findings indicate that stress triggers a peculiar timing response in the MMP-9 levels, net activity, and subcellular distribution in the hippocampus, suggesting its involvement in the processing of substrates during the stress response.

AB - A single stress exposure facilitates memory formation through neuroplastic processes that reshape excitatory synapses in the hippocampus, probably requiring changes in extracellular matrix components. We tested the hypothesis that matrix metalloproteinase 9 (MMP-9), an enzyme that degrades components of extracellular matrix and synaptic proteins such as β-dystroglycan (β-DG43), changes their activity and distribution in rat hippocampus during the acute stress response. After 2.5 h of restraint stress, we found (i) increased MMP-9 levels and potential activity in whole hippocampal extracts, accompanied by β-DG43 cleavage, and (ii) a significant enhancement of MMP-9 immunoreactivity in dendritic fields such as stratum radiatum and the molecular layer of hippocampus. After 24 h of stress, we found that (i) MMP-9 net activity rises at somatic field, i.e., stratum pyramidale and granule cell layers, and also at synaptic field, mainly stratum radiatum and the molecular layer of hippocampus, and (ii) hippocampal synaptoneurosome fractions are enriched with MMP-9, without variation of its potential enzymatic activity, in accordance with the constant level of cleaved β-DG43. These findings indicate that stress triggers a peculiar timing response in the MMP-9 levels, net activity, and subcellular distribution in the hippocampus, suggesting its involvement in the processing of substrates during the stress response.

KW - Acute stress

KW - Hippocampus

KW - MMP-9

KW - Synaptoneurosomes

KW - Zymography

KW - β-Dystroglycan

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DO - 10.1021/acschemneuro.7b00387

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