Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora

Augusto Manubens, Paulo Canessa, Carolina Folch, Marcela Avila, Loreto Salas, Rafael Vicuña

Resultado de la investigación: Article

29 Citas (Scopus)

Resumen

The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0-10 μM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160-194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag +, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn 2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.

Idioma originalEnglish
Páginas (desde-hasta)139-145
Número de páginas7
PublicaciónFEMS Microbiology Letters
Volumen275
N.º1
DOI
EstadoPublished - 1 oct 2007

Huella dactilar

Coriolaceae
Laccase
Basidiomycota
Manganese
Mycelium
Messenger RNA
Lignin
Growth
Fungi
Metals
Ions
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Citar esto

Manubens, Augusto ; Canessa, Paulo ; Folch, Carolina ; Avila, Marcela ; Salas, Loreto ; Vicuña, Rafael. / Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora. En: FEMS Microbiology Letters. 2007 ; Vol. 275, N.º 1. pp. 139-145.
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Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora. / Manubens, Augusto; Canessa, Paulo; Folch, Carolina; Avila, Marcela; Salas, Loreto; Vicuña, Rafael.

En: FEMS Microbiology Letters, Vol. 275, N.º 1, 01.10.2007, p. 139-145.

Resultado de la investigación: Article

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T1 - Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora

AU - Manubens, Augusto

AU - Canessa, Paulo

AU - Folch, Carolina

AU - Avila, Marcela

AU - Salas, Loreto

AU - Vicuña, Rafael

PY - 2007/10/1

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N2 - The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0-10 μM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160-194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag +, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn 2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.

AB - The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0-10 μM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160-194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag +, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn 2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.

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