Resumen
1.An increasing body of knowledge suggests that the estimation of critical upper thermal limits (CTmax) is highly dependent on the experimental methodology employed. Here, we employ a theoretical approach to analyse how estimates of CTmax (knock-down temperatures and times) are affected by measurement protocol. 2.Our model is able to reproduce the results of empirical studies on Drosophila melanogaster, suggesting that it adequately mimics organismal responses during assays. With simulated data sets, we also show that many experimental protocols result in unreliable and often highly biased estimates of CTmax in Drosophila and possibly in other ectotherms. 3.The confounding effects of stochasticity, resource depletion (or fatigue) and short-term acclimatory responses are expected to be higher in longer assays, and therefore, short assays should be generally preferred. The experimental protocol of choice must also take into consideration the range in which measurement accuracy is not affected and the potential problems of thermal inertia in larger organisms. 4.Our findings justify previous concerns that the methodology may have a greater impact on estimates of CTmax than the biological process under study, and explain why many studies on the subject have often reported inconsistent and even contradictory results.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 1169-1180 |
| Número de páginas | 12 |
| Publicación | Functional Ecology |
| Volumen | 25 |
| N.º | 6 |
| DOI | |
| Estado | Published - dic 2011 |
Huella dactilar
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
Citar esto
}
Making sense of heat tolerance estimates in ectotherms : Lessons from Drosophila. / Santos, Mauro; Castañeda, Luis E.; Rezende, Enrico L.
En: Functional Ecology, Vol. 25, N.º 6, 12.2011, p. 1169-1180.Resultado de la investigación: Article
TY - JOUR
T1 - Making sense of heat tolerance estimates in ectotherms
T2 - Lessons from Drosophila
AU - Santos, Mauro
AU - Castañeda, Luis E.
AU - Rezende, Enrico L.
PY - 2011/12
Y1 - 2011/12
N2 - 1.An increasing body of knowledge suggests that the estimation of critical upper thermal limits (CTmax) is highly dependent on the experimental methodology employed. Here, we employ a theoretical approach to analyse how estimates of CTmax (knock-down temperatures and times) are affected by measurement protocol. 2.Our model is able to reproduce the results of empirical studies on Drosophila melanogaster, suggesting that it adequately mimics organismal responses during assays. With simulated data sets, we also show that many experimental protocols result in unreliable and often highly biased estimates of CTmax in Drosophila and possibly in other ectotherms. 3.The confounding effects of stochasticity, resource depletion (or fatigue) and short-term acclimatory responses are expected to be higher in longer assays, and therefore, short assays should be generally preferred. The experimental protocol of choice must also take into consideration the range in which measurement accuracy is not affected and the potential problems of thermal inertia in larger organisms. 4.Our findings justify previous concerns that the methodology may have a greater impact on estimates of CTmax than the biological process under study, and explain why many studies on the subject have often reported inconsistent and even contradictory results.
AB - 1.An increasing body of knowledge suggests that the estimation of critical upper thermal limits (CTmax) is highly dependent on the experimental methodology employed. Here, we employ a theoretical approach to analyse how estimates of CTmax (knock-down temperatures and times) are affected by measurement protocol. 2.Our model is able to reproduce the results of empirical studies on Drosophila melanogaster, suggesting that it adequately mimics organismal responses during assays. With simulated data sets, we also show that many experimental protocols result in unreliable and often highly biased estimates of CTmax in Drosophila and possibly in other ectotherms. 3.The confounding effects of stochasticity, resource depletion (or fatigue) and short-term acclimatory responses are expected to be higher in longer assays, and therefore, short assays should be generally preferred. The experimental protocol of choice must also take into consideration the range in which measurement accuracy is not affected and the potential problems of thermal inertia in larger organisms. 4.Our findings justify previous concerns that the methodology may have a greater impact on estimates of CTmax than the biological process under study, and explain why many studies on the subject have often reported inconsistent and even contradictory results.
KW - Critical thermal limits
KW - CT
KW - Genetic correlation
KW - Gompertz equation
KW - Repeatability
KW - Survival probability
KW - Thermotolerance
UR - http://www.scopus.com/inward/record.url?scp=80855139853&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2435.2011.01908.x
DO - 10.1111/j.1365-2435.2011.01908.x
M3 - Article
AN - SCOPUS:80855139853
VL - 25
SP - 1169
EP - 1180
JO - Functional Ecology
JF - Functional Ecology
SN - 0269-8463
IS - 6
ER -