Localization of the 16S mitochondrial rRNA in the nucleus of mammalian spermatogenic cells

Jaime Villegas, Pamela Araya, Eduardo Bustos-Obregon, Luis O. Burzio

Resultado de la investigación: Article

30 Citas (Scopus)

Resumen

Amplification of RNA from human sperm heads yielded a fragment of 435 bp that shares 100% identity with a central region of the 16S mitochondrial rRNA. The nuclear localization in the sperm of the mitochondrial RNA was confirmed by in-situ hybridization. These results, together with the localization of the 16S mitochondrial rRNA in mouse sperm, are the first demonstration that the organelle transcript is a normal component of the mammalian gamete. The possibility that the nuclear mitochondrial RNA arises from nuclear transcription of a mitochondrial pseudogene was ruled out. To determine when during spermatogenesis the mitochondrial RNA is localized in the nucleus, in-situ hybridization of mouse and human testis was carried out. The nuclei of spermatogonia, spermatocytes and round and elongated spermatids were all positively stained. In human spermatocytes, the nuclear staining pattern was fibrillar, suggesting an association of the mitochondrial transcript with the meiotic chromosomes. These results indicate that early in spermatogenesis and before the onset of meiosis, the 16S mitochondrial rRNA is localized in the nucleus of spermatogenic cells, suggesting a process of translocation of the transcript from the mitochondria.

Idioma originalEnglish
Páginas (desde-hasta)977-983
Número de páginas7
PublicaciónMolecular Human Reproduction
Volumen8
N.º11
EstadoPublished - 1 nov 2002

Huella dactilar

Spermatocytes
Spermatogenesis
In Situ Hybridization
Spermatozoa
Sperm Head
Nuclear RNA
Spermatogonia
Pseudogenes
Spermatids
Meiosis
Cell Nucleus
Germ Cells
Organelles
Testis
Mitochondria
Chromosomes
RNA
Staining and Labeling
mitochondrial RNA

ASJC Scopus subject areas

  • Medicine(all)
  • Embryology
  • Reproductive Medicine
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Citar esto

Villegas, Jaime ; Araya, Pamela ; Bustos-Obregon, Eduardo ; Burzio, Luis O. / Localization of the 16S mitochondrial rRNA in the nucleus of mammalian spermatogenic cells. En: Molecular Human Reproduction. 2002 ; Vol. 8, N.º 11. pp. 977-983.
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abstract = "Amplification of RNA from human sperm heads yielded a fragment of 435 bp that shares 100{\%} identity with a central region of the 16S mitochondrial rRNA. The nuclear localization in the sperm of the mitochondrial RNA was confirmed by in-situ hybridization. These results, together with the localization of the 16S mitochondrial rRNA in mouse sperm, are the first demonstration that the organelle transcript is a normal component of the mammalian gamete. The possibility that the nuclear mitochondrial RNA arises from nuclear transcription of a mitochondrial pseudogene was ruled out. To determine when during spermatogenesis the mitochondrial RNA is localized in the nucleus, in-situ hybridization of mouse and human testis was carried out. The nuclei of spermatogonia, spermatocytes and round and elongated spermatids were all positively stained. In human spermatocytes, the nuclear staining pattern was fibrillar, suggesting an association of the mitochondrial transcript with the meiotic chromosomes. These results indicate that early in spermatogenesis and before the onset of meiosis, the 16S mitochondrial rRNA is localized in the nucleus of spermatogenic cells, suggesting a process of translocation of the transcript from the mitochondria.",
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Localization of the 16S mitochondrial rRNA in the nucleus of mammalian spermatogenic cells. / Villegas, Jaime; Araya, Pamela; Bustos-Obregon, Eduardo; Burzio, Luis O.

En: Molecular Human Reproduction, Vol. 8, N.º 11, 01.11.2002, p. 977-983.

Resultado de la investigación: Article

TY - JOUR

T1 - Localization of the 16S mitochondrial rRNA in the nucleus of mammalian spermatogenic cells

AU - Villegas, Jaime

AU - Araya, Pamela

AU - Bustos-Obregon, Eduardo

AU - Burzio, Luis O.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Amplification of RNA from human sperm heads yielded a fragment of 435 bp that shares 100% identity with a central region of the 16S mitochondrial rRNA. The nuclear localization in the sperm of the mitochondrial RNA was confirmed by in-situ hybridization. These results, together with the localization of the 16S mitochondrial rRNA in mouse sperm, are the first demonstration that the organelle transcript is a normal component of the mammalian gamete. The possibility that the nuclear mitochondrial RNA arises from nuclear transcription of a mitochondrial pseudogene was ruled out. To determine when during spermatogenesis the mitochondrial RNA is localized in the nucleus, in-situ hybridization of mouse and human testis was carried out. The nuclei of spermatogonia, spermatocytes and round and elongated spermatids were all positively stained. In human spermatocytes, the nuclear staining pattern was fibrillar, suggesting an association of the mitochondrial transcript with the meiotic chromosomes. These results indicate that early in spermatogenesis and before the onset of meiosis, the 16S mitochondrial rRNA is localized in the nucleus of spermatogenic cells, suggesting a process of translocation of the transcript from the mitochondria.

AB - Amplification of RNA from human sperm heads yielded a fragment of 435 bp that shares 100% identity with a central region of the 16S mitochondrial rRNA. The nuclear localization in the sperm of the mitochondrial RNA was confirmed by in-situ hybridization. These results, together with the localization of the 16S mitochondrial rRNA in mouse sperm, are the first demonstration that the organelle transcript is a normal component of the mammalian gamete. The possibility that the nuclear mitochondrial RNA arises from nuclear transcription of a mitochondrial pseudogene was ruled out. To determine when during spermatogenesis the mitochondrial RNA is localized in the nucleus, in-situ hybridization of mouse and human testis was carried out. The nuclei of spermatogonia, spermatocytes and round and elongated spermatids were all positively stained. In human spermatocytes, the nuclear staining pattern was fibrillar, suggesting an association of the mitochondrial transcript with the meiotic chromosomes. These results indicate that early in spermatogenesis and before the onset of meiosis, the 16S mitochondrial rRNA is localized in the nucleus of spermatogenic cells, suggesting a process of translocation of the transcript from the mitochondria.

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KW - Mitochondrial RNA

KW - Mouse

KW - Sperm

KW - Spermatogenesis

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