Limited life cycle and cost assessment for the bioconversion of lignin-derived aromatics into adipic acid

Jozef B.J.H. van Duuren, Paul J. de Wild, Sören Starck, Christian Bradtmöller, Mirjam Selzer, Kerstin Mehlmann, Roland Schneider, Michael Kohlstedt, Ignacio Poblete-Castro, Jessica Stolzenberger, Nadja Barton, Michel Fritz, Stephan Scholl, Joachim Venus, Christoph Wittmann

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)


Lignin is an abundant and heterogeneous waste byproduct of the cellulosic industry, which has the potential of being transformed into valuable biochemicals via microbial fermentation. In this study, we applied a fast-pyrolysis process using softwood lignin resulting in a two-phase bio-oil containing monomeric and oligomeric aromatics without syringol. We demonstrated that an additional hydrodeoxygenation step within the process leads to an enhanced thermochemical conversion of guaiacol into catechol and phenol. After steam bath distillation, Pseudomonas putida KT2440-BN6 achieved a percent yield of cis, cis-muconic acid of up to 95 mol% from catechol derived from the aqueous phase. We next established a downstream process for purifying cis, cis-muconic acid (39.9 g/L) produced in a 42.5 L fermenter using glucose and benzoate as carbon substrates. On the basis of the obtained values for each unit operation of the empirical processes, we next performed a limited life cycle and cost analysis of an integrated biotechnological and chemical process for producing adipic acid and then compared it with the conventional petrochemical route. The simulated scenarios estimate that by attaining a mixture of catechol, phenol, cresol, and guaiacol (1:0.34:0.18:0, mol ratio), a titer of 62.5 (g/L) cis, cis-muconic acid in the bioreactor, and a controlled cooling of pyrolysis gases to concentrate monomeric aromatics in the aqueous phase, the bio-based route results in a reduction of CO2-eq emission by 58% and energy demand by 23% with a contribution margin for the aqueous phase of up to 88.05 euro/ton. We conclude that the bio-based production of adipic acid from softwood lignins brings environmental benefits over the petrochemical procedure and is cost-effective at an industrial scale. Further research is essential to achieve the proposed cis, cis-muconic acid yield from true lignin-derived aromatics using whole-cell biocatalysts.

Idioma originalInglés
Páginas (desde-hasta)1381-1393
Número de páginas13
PublicaciónBiotechnology and Bioengineering
EstadoEn prensa - 1 ene 2020

Áreas temáticas de ASJC Scopus

  • Biotecnología
  • Bioingeniería
  • Microbiología y biotecnología aplicadas


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