Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters

Julian Bejarano, Aldo Rojas, Andrea Ramírez-Sagredo, Ana L. Riveros, Francisco Morales-Zavala, Yvo Flores, Jaime A. Riquelme, Fanny Guzman, Eyleen Araya, Mario Chiong, María Paz Ocaranza, Javier O. Morales, María Gabriela Villamizar Sarmiento, Gina Sanchez, Sergio Lavandero, Marcelo J. Kogan

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoTherm-AuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 μM. This angiotensin-(1–9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43 °C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNC-based nanosystem was confirmed using an ex vivo Langendorff heart model.

Idioma originalInglés
Páginas (desde-hasta)859-872
Número de páginas14
PublicaciónJournal of Controlled Release
Volumen328
DOI
EstadoPublicada - 10 dic 2020

Áreas temáticas de ASJC Scopus

  • Ciencias farmacéuticas

Huella Profundice en los temas de investigación de 'Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters'. En conjunto forman una huella única.

Citar esto