TY - JOUR
T1 - Labelling fish diets with 15N -Leucine for monitoring feed consumption and bio-distribution in Atlantic salmon
AU - Cortés-Quezada, Mirta
AU - Parada, Ana María
AU - Videla, Ximena
AU - Valdés, Juan Antonio
AU - Gonzalez-Catrilelbún, Sebastián
AU - Aspée, Alexis
AU - Nario, Adriana
AU - Rivas-Aravena, Andrea
N1 - Publisher Copyright:
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - Feeding represents 50–70% of the cost of production in salmon farming, higher than any other animal farm. The improvement of this percentage is challenging as the food is thrown into the fish tank, there is no quantification of the amount of food that is consumed by the fish. In consequence, it is difficult to adjust the food composition making it more nutritive or promoting food consumption by fish. In this study, to investigate food consumption, bio-distribution and food residues, leucine containing 15N (a stable isotope of nitrogen) was used to label the fish food. Atlantic salmon (Salmo salar) weighing 100–120 g were maintained in 30 L tanks at a density of 14 kg/m3. Fishes were fed daily at 1% of the fish weight with pellet labelled with 15N-leucine. The 15N incorporation was determined 14 hours after the feeding in all the fish organs. Results showed that 14 hours after the administration of a single dose of labelled food to Atlantic salmon enables the detection of the tracer in the whole organism allowing determining the food consumption. Through the analysis of nitrogen use efficiency (NUE), we showed that the trunk, pyloric caeca and head incorporate the highest level of the marker (72.7, 8.7 and 5.7%, respectively). This methodology would permit monitoring feeding to minimize food loss, improve administration methodologies or select the preferred foods for the fish, among others to reduce production costs.
AB - Feeding represents 50–70% of the cost of production in salmon farming, higher than any other animal farm. The improvement of this percentage is challenging as the food is thrown into the fish tank, there is no quantification of the amount of food that is consumed by the fish. In consequence, it is difficult to adjust the food composition making it more nutritive or promoting food consumption by fish. In this study, to investigate food consumption, bio-distribution and food residues, leucine containing 15N (a stable isotope of nitrogen) was used to label the fish food. Atlantic salmon (Salmo salar) weighing 100–120 g were maintained in 30 L tanks at a density of 14 kg/m3. Fishes were fed daily at 1% of the fish weight with pellet labelled with 15N-leucine. The 15N incorporation was determined 14 hours after the feeding in all the fish organs. Results showed that 14 hours after the administration of a single dose of labelled food to Atlantic salmon enables the detection of the tracer in the whole organism allowing determining the food consumption. Through the analysis of nitrogen use efficiency (NUE), we showed that the trunk, pyloric caeca and head incorporate the highest level of the marker (72.7, 8.7 and 5.7%, respectively). This methodology would permit monitoring feeding to minimize food loss, improve administration methodologies or select the preferred foods for the fish, among others to reduce production costs.
KW - feeding in aquaculture
KW - fish feeding
KW - food marker
KW - nitrogen biodistribution
KW - oral intake
UR - http://www.scopus.com/inward/record.url?scp=85127274680&partnerID=8YFLogxK
U2 - 10.1002/vms3.730
DO - 10.1002/vms3.730
M3 - Article
C2 - 35348306
AN - SCOPUS:85127274680
SN - 2053-1095
VL - 8
SP - 1096
EP - 1103
JO - Veterinary Medicine and Science
JF - Veterinary Medicine and Science
IS - 3
ER -