Kinetics of copper accumulation in Lessonia nigrescens (Phaeophyceae) under conditions of environmental oxidative stress

Santiago Andrade, Loretto Contreras, James W. Moffett, Juan A. Correa

Resultado de la investigación: Article

33 Citas (Scopus)

Resumen

Juvenile individuals of the brown kelp Lessonia nigrescens were exposed to a coastal environment chronically impacted by copper mine wastes and currently displaying more than 250 nM of total dissolved copper. The kinetic of copper accumulation in the intra and extracellular compartments was determined and correlated to the oxidative burst resulting from copper-mediated oxidative stress. Accumulation involved an initial adsorption onto the outer cell wall followed by a slower uptake into the cells. A linear pattern of copper uptake over time was found during the first 52 h of exposure, and a steady state was reached at 76 h. The resulting oxidative stress was found to be inefficiently attenuated, and the intracellular level of copper remained sufficiently high to determine a persistently higher than normal level of reactive oxygen species (ROS). Thus, our results strongly suggest that, in L. nigrescens, copper needs to reach an intracellular threshold before oxidative burst develops. Furthermore, it was found that the high ROS levels generated by copper accumulation within the cells persists after the oxidative burst has ceased, suggesting a limited capacity of the algal tissue to buffer the increases of ROS caused by the environmental copper levels.

Idioma originalEnglish
Páginas (desde-hasta)398-401
Número de páginas4
PublicaciónAquatic Toxicology
Volumen78
N.º4
DOI
EstadoPublished - 20 jul 2006

Huella dactilar

Phaeophyta
Phaeophyceae
Copper
Oxidative Stress
oxidative stress
copper
kinetics
environmental factors
Respiratory Burst
reactive oxygen species
Reactive Oxygen Species
Kelp
uptake mechanisms
Lessonia nigrescens
mine waste
Cell Wall
Adsorption
coastal zone
macroalgae
Buffers

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Citar esto

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Kinetics of copper accumulation in Lessonia nigrescens (Phaeophyceae) under conditions of environmental oxidative stress. / Andrade, Santiago; Contreras, Loretto; Moffett, James W.; Correa, Juan A.

En: Aquatic Toxicology, Vol. 78, N.º 4, 20.07.2006, p. 398-401.

Resultado de la investigación: Article

TY - JOUR

T1 - Kinetics of copper accumulation in Lessonia nigrescens (Phaeophyceae) under conditions of environmental oxidative stress

AU - Andrade, Santiago

AU - Contreras, Loretto

AU - Moffett, James W.

AU - Correa, Juan A.

PY - 2006/7/20

Y1 - 2006/7/20

N2 - Juvenile individuals of the brown kelp Lessonia nigrescens were exposed to a coastal environment chronically impacted by copper mine wastes and currently displaying more than 250 nM of total dissolved copper. The kinetic of copper accumulation in the intra and extracellular compartments was determined and correlated to the oxidative burst resulting from copper-mediated oxidative stress. Accumulation involved an initial adsorption onto the outer cell wall followed by a slower uptake into the cells. A linear pattern of copper uptake over time was found during the first 52 h of exposure, and a steady state was reached at 76 h. The resulting oxidative stress was found to be inefficiently attenuated, and the intracellular level of copper remained sufficiently high to determine a persistently higher than normal level of reactive oxygen species (ROS). Thus, our results strongly suggest that, in L. nigrescens, copper needs to reach an intracellular threshold before oxidative burst develops. Furthermore, it was found that the high ROS levels generated by copper accumulation within the cells persists after the oxidative burst has ceased, suggesting a limited capacity of the algal tissue to buffer the increases of ROS caused by the environmental copper levels.

AB - Juvenile individuals of the brown kelp Lessonia nigrescens were exposed to a coastal environment chronically impacted by copper mine wastes and currently displaying more than 250 nM of total dissolved copper. The kinetic of copper accumulation in the intra and extracellular compartments was determined and correlated to the oxidative burst resulting from copper-mediated oxidative stress. Accumulation involved an initial adsorption onto the outer cell wall followed by a slower uptake into the cells. A linear pattern of copper uptake over time was found during the first 52 h of exposure, and a steady state was reached at 76 h. The resulting oxidative stress was found to be inefficiently attenuated, and the intracellular level of copper remained sufficiently high to determine a persistently higher than normal level of reactive oxygen species (ROS). Thus, our results strongly suggest that, in L. nigrescens, copper needs to reach an intracellular threshold before oxidative burst develops. Furthermore, it was found that the high ROS levels generated by copper accumulation within the cells persists after the oxidative burst has ceased, suggesting a limited capacity of the algal tissue to buffer the increases of ROS caused by the environmental copper levels.

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