IST2

An insertion sequence from Thiobacillus ferrooxidans

J. R. Yates, R. P. Cunningham, D. S. Holmes

Resultado de la investigación: Article

32 Citas (Scopus)

Resumen

The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.

Idioma originalEnglish
Páginas (desde-hasta)7284-7287
Número de páginas4
PublicaciónProceedings of the National Academy of Sciences of the United States of America
Volumen85
N.º19
EstadoPublished - 1988

Huella dactilar

Thiobacillus
Insertional Mutagenesis
Base Pairing
DNA Transposable Elements
Terminal Repeat Sequences
Open Reading Frames
Genome

ASJC Scopus subject areas

  • General
  • Genetics

Citar esto

Yates, J. R. ; Cunningham, R. P. ; Holmes, D. S. / IST2 : An insertion sequence from Thiobacillus ferrooxidans. En: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, N.º 19. pp. 7284-7287.
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IST2 : An insertion sequence from Thiobacillus ferrooxidans. / Yates, J. R.; Cunningham, R. P.; Holmes, D. S.

En: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, N.º 19, 1988, p. 7284-7287.

Resultado de la investigación: Article

TY - JOUR

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T2 - An insertion sequence from Thiobacillus ferrooxidans

AU - Yates, J. R.

AU - Cunningham, R. P.

AU - Holmes, D. S.

PY - 1988

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N2 - The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.

AB - The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.

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