Isolation of Flavobacterium-like bacteria from diseased salmonids cultured in Chile

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

The biochemical, serological and molecular characteristics of 8-pigmented isolates phenotypically related to Flavobacterium psychrophilum were determined. The bacteria were isolated in 2006 from diseased Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) cultured in the South of Chile. The F. psychrophilum type strain ATCC 49418T and isolate B97026, obtained from rainbow trout in UK, were included in the study as a comparison. Their potential to cause pathology in Atlantic salmon was also investigated. The 8 isolates were biochemically identical, however were completely different to F. psychrophilum. Slide agglutination and Dot blot allowed us to confirm that the isolates constituted a homogeneous, but distinctive serological group to the F. psychrophilum isolates. Similar homogeneity was observed using RAPD-PCR with identical DNA patterns obtained, regardless of the source of isolation or geographic origin. Therefore, any of the above tests can be used to discriminate these pigmented bacteria from F. psychrophilum. Virulence studies suggest that these isolates could be considered as a potential pathogen for salmonids, mainly in mixed cultures with F. psychrophilum. These fish showed typical signs of flavobacteriosis. Recent studies on the sequencing of the 16S rRNA gene, together with the phenotypic and biochemical properties obtained in this study permitted us classify these 8 isolates in the genus Chryseobacterium. Further molecular studies are in progress in order to know the species of these pigmented bacteria and determine the real risk for the salmonid culture.

Idioma originalEnglish
Páginas (desde-hasta)176-185
Número de páginas10
PublicaciónBulletin of the European Association of Fish Pathologists
Volumen28
N.º5
EstadoPublished - 2008

Huella dactilar

Flavobacterium psychrophilum
Salmonidae
Flavobacterium
Salmo salar
Chile
Oncorhynchus mykiss
Bacteria
bacterium
rainbow
salmonid culture
bacteria
Chryseobacterium
Random Amplified Polymorphic DNA Technique
Agglutination
pathology
virulence
rRNA Genes
homogeneity
Virulence
Fishes

ASJC Scopus subject areas

  • Aquatic Science
  • Small Animals

Citar esto

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title = "Isolation of Flavobacterium-like bacteria from diseased salmonids cultured in Chile",
abstract = "The biochemical, serological and molecular characteristics of 8-pigmented isolates phenotypically related to Flavobacterium psychrophilum were determined. The bacteria were isolated in 2006 from diseased Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) cultured in the South of Chile. The F. psychrophilum type strain ATCC 49418T and isolate B97026, obtained from rainbow trout in UK, were included in the study as a comparison. Their potential to cause pathology in Atlantic salmon was also investigated. The 8 isolates were biochemically identical, however were completely different to F. psychrophilum. Slide agglutination and Dot blot allowed us to confirm that the isolates constituted a homogeneous, but distinctive serological group to the F. psychrophilum isolates. Similar homogeneity was observed using RAPD-PCR with identical DNA patterns obtained, regardless of the source of isolation or geographic origin. Therefore, any of the above tests can be used to discriminate these pigmented bacteria from F. psychrophilum. Virulence studies suggest that these isolates could be considered as a potential pathogen for salmonids, mainly in mixed cultures with F. psychrophilum. These fish showed typical signs of flavobacteriosis. Recent studies on the sequencing of the 16S rRNA gene, together with the phenotypic and biochemical properties obtained in this study permitted us classify these 8 isolates in the genus Chryseobacterium. Further molecular studies are in progress in order to know the species of these pigmented bacteria and determine the real risk for the salmonid culture.",
author = "P. Ilardi and R. Avenda{\~n}o-Herrera",
year = "2008",
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pages = "176--185",
journal = "Bulletin of the European Association of Fish Pathologists",
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TY - JOUR

T1 - Isolation of Flavobacterium-like bacteria from diseased salmonids cultured in Chile

AU - Ilardi, P.

AU - Avendaño-Herrera, R.

PY - 2008

Y1 - 2008

N2 - The biochemical, serological and molecular characteristics of 8-pigmented isolates phenotypically related to Flavobacterium psychrophilum were determined. The bacteria were isolated in 2006 from diseased Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) cultured in the South of Chile. The F. psychrophilum type strain ATCC 49418T and isolate B97026, obtained from rainbow trout in UK, were included in the study as a comparison. Their potential to cause pathology in Atlantic salmon was also investigated. The 8 isolates were biochemically identical, however were completely different to F. psychrophilum. Slide agglutination and Dot blot allowed us to confirm that the isolates constituted a homogeneous, but distinctive serological group to the F. psychrophilum isolates. Similar homogeneity was observed using RAPD-PCR with identical DNA patterns obtained, regardless of the source of isolation or geographic origin. Therefore, any of the above tests can be used to discriminate these pigmented bacteria from F. psychrophilum. Virulence studies suggest that these isolates could be considered as a potential pathogen for salmonids, mainly in mixed cultures with F. psychrophilum. These fish showed typical signs of flavobacteriosis. Recent studies on the sequencing of the 16S rRNA gene, together with the phenotypic and biochemical properties obtained in this study permitted us classify these 8 isolates in the genus Chryseobacterium. Further molecular studies are in progress in order to know the species of these pigmented bacteria and determine the real risk for the salmonid culture.

AB - The biochemical, serological and molecular characteristics of 8-pigmented isolates phenotypically related to Flavobacterium psychrophilum were determined. The bacteria were isolated in 2006 from diseased Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) cultured in the South of Chile. The F. psychrophilum type strain ATCC 49418T and isolate B97026, obtained from rainbow trout in UK, were included in the study as a comparison. Their potential to cause pathology in Atlantic salmon was also investigated. The 8 isolates were biochemically identical, however were completely different to F. psychrophilum. Slide agglutination and Dot blot allowed us to confirm that the isolates constituted a homogeneous, but distinctive serological group to the F. psychrophilum isolates. Similar homogeneity was observed using RAPD-PCR with identical DNA patterns obtained, regardless of the source of isolation or geographic origin. Therefore, any of the above tests can be used to discriminate these pigmented bacteria from F. psychrophilum. Virulence studies suggest that these isolates could be considered as a potential pathogen for salmonids, mainly in mixed cultures with F. psychrophilum. These fish showed typical signs of flavobacteriosis. Recent studies on the sequencing of the 16S rRNA gene, together with the phenotypic and biochemical properties obtained in this study permitted us classify these 8 isolates in the genus Chryseobacterium. Further molecular studies are in progress in order to know the species of these pigmented bacteria and determine the real risk for the salmonid culture.

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