Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum

Pamela Machuca, Leslie Daille, Enrique Vinés, Liliana Berrocal, Mauricio Bittner

Resultado de la investigación: Article

13 Citas (Scopus)

Resumen

Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (FnpΦ02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded DNA. The morphological features and the genetic characteristics suggest that FnpΦ02 is part of the Siphoviridae family. Using one-step growth and adsorption experiments, the latent period, burst size, and adsorption rate were estimated to be 15 h, 100 infectious units per cell, and 7.5 × 10-10 ml min-1, respectively. A small fragment of phage DNA was cloned and sequenced, showing 93% nucleotide identity with the phage PA6 of Propionibacterium acnes and amino acid identity with fragments of two proteins (Gp3 and Gp4) of this phage. To our knowledge, FnpΦ02 is the first phage described to infect Fusobacterium nucleatum and provides the base for future exploration of phages in the control of periodontal disease.

Idioma originalEnglish
Páginas (desde-hasta)7243-7250
Número de páginas8
PublicaciónApplied and Environmental Microbiology
Volumen76
N.º21
DOI
EstadoPublished - nov 2010

Huella dactilar

Fusobacterium nucleatum
bacteriophage
bacteriophages
Bacteriophages
pathogen
adsorption
DNA
pathogens
pathology
tooth
transmission electron microscopy
genome
amino acid
Periodontal Diseases
bacterium
protein
Adsorption
Siphoviridae
Propionibacterium acnes
experiment

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Citar esto

Machuca, Pamela ; Daille, Leslie ; Vinés, Enrique ; Berrocal, Liliana ; Bittner, Mauricio. / Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum. En: Applied and Environmental Microbiology. 2010 ; Vol. 76, N.º 21. pp. 7243-7250.
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Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum. / Machuca, Pamela; Daille, Leslie; Vinés, Enrique; Berrocal, Liliana; Bittner, Mauricio.

En: Applied and Environmental Microbiology, Vol. 76, N.º 21, 11.2010, p. 7243-7250.

Resultado de la investigación: Article

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AU - Bittner, Mauricio

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