Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica

Felipe A. Arenas, Benoit Pugin, Nicole A. Henríquez, Mauricio A. Arenas-Salinas, Waldo A. Díaz-Vásquez, María F. Pozo, Claudia M. Muñoz, Thomas G. Chasteen, José M. Pérez-Donoso, Claudio C. Vásquez

Resultado de la investigación: Article

22 Citas (Scopus)

Resumen

The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance is the enzymatic and non-enzymatic reduction of tellurite to the less toxic elemental tellurium. This reduction generates nano- to micrometric tellurium crystals that display different shapes and sizes.To date, a very limited number of highly tellurite-resistant and tellurite-reducing bacterial species are available from international culture collections. In this work, we decided to look for tellurite-reducing bacteria from an extreme environment, Antarctica. This environment exhibits a combination of several extreme factors such as high UV-radiation and desiccation and freezing conditions that impact directly on the local biodiversity. Since, as does, all these factors induce ROS formation, we hypothesized that Antarctic bacteria could also exhibit tellurite-resistance. In this context, we isolated 123 tellurite-resistant bacteria, and characterized six new tellurite-resistant and tellurite-reducing bacterial strains from samples collected in Antarctica. These strains were identified according to their 16S rRNA gene sequence as Staphylococcus hameolyticus, Staphylococcus sciuri, Acinetobacter haemolyticus, Pseudomonas lini, and two strains of Psychrobacter immobilis.The isolates display tellurite-resistance about 35- to 500-fold higher than Escherichia coli (Te-sensitive organism), and a high level of tellurite reduction which might be interesting for an application in the field of bioremediation or nanoparticle biosynthesis.

Idioma originalEnglish
Páginas (desde-hasta)40-52
Número de páginas13
PublicaciónPolar Science
Volumen8
N.º1
DOI
EstadoPublished - 1 mar 2014

Huella dactilar

tellurite
tellurium
Antarctica
bacterium
bacteria
Pseudomonas lini
Acinetobacter haemolyticus
Psychrobacter immobilis
reactive oxygen species
Staphylococcus sciuri
organisms
bioremediation
nanoparticles
Staphylococcus
thiols
resistance mechanisms
ultraviolet radiation
crystals
freezing
ribosomal RNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology
  • Earth and Planetary Sciences(all)

Citar esto

Arenas, F. A., Pugin, B., Henríquez, N. A., Arenas-Salinas, M. A., Díaz-Vásquez, W. A., Pozo, M. F., ... Vásquez, C. C. (2014). Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica. Polar Science, 8(1), 40-52. https://doi.org/10.1016/j.polar.2014.01.001
Arenas, Felipe A. ; Pugin, Benoit ; Henríquez, Nicole A. ; Arenas-Salinas, Mauricio A. ; Díaz-Vásquez, Waldo A. ; Pozo, María F. ; Muñoz, Claudia M. ; Chasteen, Thomas G. ; Pérez-Donoso, José M. ; Vásquez, Claudio C. / Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica. En: Polar Science. 2014 ; Vol. 8, N.º 1. pp. 40-52.
@article{11ad94a0da1a40bfaf918ce0f248602b,
title = "Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica",
abstract = "The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance is the enzymatic and non-enzymatic reduction of tellurite to the less toxic elemental tellurium. This reduction generates nano- to micrometric tellurium crystals that display different shapes and sizes.To date, a very limited number of highly tellurite-resistant and tellurite-reducing bacterial species are available from international culture collections. In this work, we decided to look for tellurite-reducing bacteria from an extreme environment, Antarctica. This environment exhibits a combination of several extreme factors such as high UV-radiation and desiccation and freezing conditions that impact directly on the local biodiversity. Since, as does, all these factors induce ROS formation, we hypothesized that Antarctic bacteria could also exhibit tellurite-resistance. In this context, we isolated 123 tellurite-resistant bacteria, and characterized six new tellurite-resistant and tellurite-reducing bacterial strains from samples collected in Antarctica. These strains were identified according to their 16S rRNA gene sequence as Staphylococcus hameolyticus, Staphylococcus sciuri, Acinetobacter haemolyticus, Pseudomonas lini, and two strains of Psychrobacter immobilis.The isolates display tellurite-resistance about 35- to 500-fold higher than Escherichia coli (Te-sensitive organism), and a high level of tellurite reduction which might be interesting for an application in the field of bioremediation or nanoparticle biosynthesis.",
keywords = "Antarctic strains, Metalloids, Tellurite reduction, Tellurite resistance, Tellurium nanostructures",
author = "Arenas, {Felipe A.} and Benoit Pugin and Henr{\'i}quez, {Nicole A.} and Arenas-Salinas, {Mauricio A.} and D{\'i}az-V{\'a}squez, {Waldo A.} and Pozo, {Mar{\'i}a F.} and Mu{\~n}oz, {Claudia M.} and Chasteen, {Thomas G.} and P{\'e}rez-Donoso, {Jos{\'e} M.} and V{\'a}squez, {Claudio C.}",
year = "2014",
month = "3",
day = "1",
doi = "10.1016/j.polar.2014.01.001",
language = "English",
volume = "8",
pages = "40--52",
journal = "Polar Science",
issn = "1873-9652",
publisher = "Elsevier",
number = "1",

}

Arenas, FA, Pugin, B, Henríquez, NA, Arenas-Salinas, MA, Díaz-Vásquez, WA, Pozo, MF, Muñoz, CM, Chasteen, TG, Pérez-Donoso, JM & Vásquez, CC 2014, 'Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica', Polar Science, vol. 8, n.º 1, pp. 40-52. https://doi.org/10.1016/j.polar.2014.01.001

Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica. / Arenas, Felipe A.; Pugin, Benoit; Henríquez, Nicole A.; Arenas-Salinas, Mauricio A.; Díaz-Vásquez, Waldo A.; Pozo, María F.; Muñoz, Claudia M.; Chasteen, Thomas G.; Pérez-Donoso, José M.; Vásquez, Claudio C.

En: Polar Science, Vol. 8, N.º 1, 01.03.2014, p. 40-52.

Resultado de la investigación: Article

TY - JOUR

T1 - Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica

AU - Arenas, Felipe A.

AU - Pugin, Benoit

AU - Henríquez, Nicole A.

AU - Arenas-Salinas, Mauricio A.

AU - Díaz-Vásquez, Waldo A.

AU - Pozo, María F.

AU - Muñoz, Claudia M.

AU - Chasteen, Thomas G.

AU - Pérez-Donoso, José M.

AU - Vásquez, Claudio C.

PY - 2014/3/1

Y1 - 2014/3/1

N2 - The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance is the enzymatic and non-enzymatic reduction of tellurite to the less toxic elemental tellurium. This reduction generates nano- to micrometric tellurium crystals that display different shapes and sizes.To date, a very limited number of highly tellurite-resistant and tellurite-reducing bacterial species are available from international culture collections. In this work, we decided to look for tellurite-reducing bacteria from an extreme environment, Antarctica. This environment exhibits a combination of several extreme factors such as high UV-radiation and desiccation and freezing conditions that impact directly on the local biodiversity. Since, as does, all these factors induce ROS formation, we hypothesized that Antarctic bacteria could also exhibit tellurite-resistance. In this context, we isolated 123 tellurite-resistant bacteria, and characterized six new tellurite-resistant and tellurite-reducing bacterial strains from samples collected in Antarctica. These strains were identified according to their 16S rRNA gene sequence as Staphylococcus hameolyticus, Staphylococcus sciuri, Acinetobacter haemolyticus, Pseudomonas lini, and two strains of Psychrobacter immobilis.The isolates display tellurite-resistance about 35- to 500-fold higher than Escherichia coli (Te-sensitive organism), and a high level of tellurite reduction which might be interesting for an application in the field of bioremediation or nanoparticle biosynthesis.

AB - The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance is the enzymatic and non-enzymatic reduction of tellurite to the less toxic elemental tellurium. This reduction generates nano- to micrometric tellurium crystals that display different shapes and sizes.To date, a very limited number of highly tellurite-resistant and tellurite-reducing bacterial species are available from international culture collections. In this work, we decided to look for tellurite-reducing bacteria from an extreme environment, Antarctica. This environment exhibits a combination of several extreme factors such as high UV-radiation and desiccation and freezing conditions that impact directly on the local biodiversity. Since, as does, all these factors induce ROS formation, we hypothesized that Antarctic bacteria could also exhibit tellurite-resistance. In this context, we isolated 123 tellurite-resistant bacteria, and characterized six new tellurite-resistant and tellurite-reducing bacterial strains from samples collected in Antarctica. These strains were identified according to their 16S rRNA gene sequence as Staphylococcus hameolyticus, Staphylococcus sciuri, Acinetobacter haemolyticus, Pseudomonas lini, and two strains of Psychrobacter immobilis.The isolates display tellurite-resistance about 35- to 500-fold higher than Escherichia coli (Te-sensitive organism), and a high level of tellurite reduction which might be interesting for an application in the field of bioremediation or nanoparticle biosynthesis.

KW - Antarctic strains

KW - Metalloids

KW - Tellurite reduction

KW - Tellurite resistance

KW - Tellurium nanostructures

UR - http://www.scopus.com/inward/record.url?scp=84894250335&partnerID=8YFLogxK

U2 - 10.1016/j.polar.2014.01.001

DO - 10.1016/j.polar.2014.01.001

M3 - Article

AN - SCOPUS:84894250335

VL - 8

SP - 40

EP - 52

JO - Polar Science

JF - Polar Science

SN - 1873-9652

IS - 1

ER -