Isolation and sequence of a rat chymotrypsin B gene

G. I. Bell, C. Quinto, M. Quiroga, P. Valenzuela, C. S. Craik, W. J. Rutter

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

99 Citas (Scopus)

Resumen

A cDNA clone encoding part of chymotrypsin B was isolated from a cDNA library prepared from rat pancreatic mRNA and used as a probe to isolate the chymotrypsin B gene. The nucleotide sequence of this gene is presented. The 4709-base pair transcribed portion of the isolated gene was inferred from the cDNA and gene sequence, and the 5' border was determined by primer extension on pancreatic polyadenylated RNA. The coding portion of the gene is interrupted by six introns. The active site residues His 57, Asp 102, and Ser 195 are encoded by separate exons. Moreover, two regions of the enzyme which form the substrate-binding pocket are also encoded by separate exons. Thus, the substrate specificity and catalytic activity of the enzyme are produced by joining several exons encoding protein segments that are intrinsically catalytically inactive. The number and location of the intron/exon junctions of the chymotrypsin gene as compared to those of other serine protease genes, as well as the location of the genes on separate chromosomes, suggest that the duplication that resulted in the formation of the chymotrypsin gene was an ancient evolutionary event.

Idioma originalInglés
Páginas (desde-hasta)14265-14270
Número de páginas6
PublicaciónJournal of Biological Chemistry
Volumen259
N.º22
EstadoPublicada - 1984

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología celular
  • Biología molecular

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