Isolation and characterization of a wound inducible phenylalanine ammonia-lyase gene (LsPAL1) from Romaine lettuce leaves

Reinaldo Campos, Hiroyuki Nonogaki, Trevor Suslow, Mikal E. Saltveit

Resultado de la investigación: Article

60 Citas (Scopus)

Resumen

Phenylalanine ammonia-lyase (PAL) catalyses the first step controlling the rate of phenylpropanoid metabolism. Wounding is a ubiquitous stress in nature and in the harvesting and preparation of fruits and vegetables that induces an increase in PAL activity, an accumulation of phenolic compounds and subsequent tissue browning. A wound-inducible PAL gene (LsPAL1) was isolated from Romaine lettuce by RT-PCR. The putative protein encoded by LsPAL1 is similar to predictive polypeptides sequences for other PALs. The kinetics of PAL mRNA accumulation is similar to those of induced PAL enzyme activity, with enzyme activity following mRNA accumulation by 12 h. Wound-induced PAL transcripts accumulated in cells close to the wound sites. Tissue printing showed that PAL mRNA was associated with tissue next to the epidermis and vascular bundles. A heterologous PAL protein was expressed in E. coli and was found to show significant PAL activity.

Idioma originalEnglish
Páginas (desde-hasta)429-438
Número de páginas10
PublicaciónPhysiologia Plantarum
Volumen121
N.º3
DOI
EstadoPublished - jul 2004

Huella dactilar

Phenylalanine Ammonia-Lyase
romaine lettuce
Lettuce
plant damage
phenylalanine ammonia-lyase
Wounds and Injuries
Genes
leaves
genes
Messenger RNA
bioprinting
enzyme activity
Printing
vascular bundles
phenylpropanoids
Enzymes
epidermis (plant)
Epidermis
Vegetables
Blood Vessels

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Physiology
  • Medicine(all)

Citar esto

Campos, Reinaldo ; Nonogaki, Hiroyuki ; Suslow, Trevor ; Saltveit, Mikal E. / Isolation and characterization of a wound inducible phenylalanine ammonia-lyase gene (LsPAL1) from Romaine lettuce leaves. En: Physiologia Plantarum. 2004 ; Vol. 121, N.º 3. pp. 429-438.
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Isolation and characterization of a wound inducible phenylalanine ammonia-lyase gene (LsPAL1) from Romaine lettuce leaves. / Campos, Reinaldo; Nonogaki, Hiroyuki; Suslow, Trevor; Saltveit, Mikal E.

En: Physiologia Plantarum, Vol. 121, N.º 3, 07.2004, p. 429-438.

Resultado de la investigación: Article

TY - JOUR

T1 - Isolation and characterization of a wound inducible phenylalanine ammonia-lyase gene (LsPAL1) from Romaine lettuce leaves

AU - Campos, Reinaldo

AU - Nonogaki, Hiroyuki

AU - Suslow, Trevor

AU - Saltveit, Mikal E.

PY - 2004/7

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AB - Phenylalanine ammonia-lyase (PAL) catalyses the first step controlling the rate of phenylpropanoid metabolism. Wounding is a ubiquitous stress in nature and in the harvesting and preparation of fruits and vegetables that induces an increase in PAL activity, an accumulation of phenolic compounds and subsequent tissue browning. A wound-inducible PAL gene (LsPAL1) was isolated from Romaine lettuce by RT-PCR. The putative protein encoded by LsPAL1 is similar to predictive polypeptides sequences for other PALs. The kinetics of PAL mRNA accumulation is similar to those of induced PAL enzyme activity, with enzyme activity following mRNA accumulation by 12 h. Wound-induced PAL transcripts accumulated in cells close to the wound sites. Tissue printing showed that PAL mRNA was associated with tissue next to the epidermis and vascular bundles. A heterologous PAL protein was expressed in E. coli and was found to show significant PAL activity.

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