Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus

Maria C. Gamboa, Susana Rasmussen-Poblete, Pablo D.T. Valenzuela, Erwin Krauskopf

Resultado de la investigación: Article

26 Citas (Scopus)

Resumen

The transcription factors CBF/DREB play an important role during low temperature, drought and high-salt stress in higher plants. In this work, we isolated one full-length CBF cDNA clone from the angiosperm Eucalyptus globulus. The derived peptide sequence reveals that it encodes a transcriptional activator that has all the characteristic motifs present in CBF proteins previously described in Arabidopsis and tomato. RT-PCR analysis shows that EgCBF1 is transiently induced in E. globulus seedlings that had been exposed to low temperature within the first 15 min. These results suggest that the isolated CBF gene participates in the cold responsive pathway of E. globulus.

Idioma originalEnglish
Páginas (desde-hasta)1-5
Número de páginas5
PublicaciónPlant Physiology and Biochemistry
Volumen45
N.º1
DOI
EstadoPublished - 1 ene 2007

Huella dactilar

Eucalyptus
Eucalyptus globulus
Transcription Factors
transcription factors
Complementary DNA
Angiosperms
Temperature
Droughts
Lycopersicon esculentum
Seedlings
Arabidopsis
salt stress
Angiospermae
temperature
Clone Cells
Salts
reverse transcriptase polymerase chain reaction
drought
tomatoes
peptides

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Citar esto

Gamboa, Maria C. ; Rasmussen-Poblete, Susana ; Valenzuela, Pablo D.T. ; Krauskopf, Erwin. / Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus. En: Plant Physiology and Biochemistry. 2007 ; Vol. 45, N.º 1. pp. 1-5.
@article{a2c9dd2f1e874ebab23a9a393cdbaddb,
title = "Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus",
abstract = "The transcription factors CBF/DREB play an important role during low temperature, drought and high-salt stress in higher plants. In this work, we isolated one full-length CBF cDNA clone from the angiosperm Eucalyptus globulus. The derived peptide sequence reveals that it encodes a transcriptional activator that has all the characteristic motifs present in CBF proteins previously described in Arabidopsis and tomato. RT-PCR analysis shows that EgCBF1 is transiently induced in E. globulus seedlings that had been exposed to low temperature within the first 15 min. These results suggest that the isolated CBF gene participates in the cold responsive pathway of E. globulus.",
keywords = "Abiotic stress, Acclimation, CBF, Cold-tolerance, Eucalyptus, Forest biotechnology",
author = "Gamboa, {Maria C.} and Susana Rasmussen-Poblete and Valenzuela, {Pablo D.T.} and Erwin Krauskopf",
year = "2007",
month = "1",
day = "1",
doi = "10.1016/j.plaphy.2006.12.006",
language = "English",
volume = "45",
pages = "1--5",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
publisher = "Elsevier Masson SAS",
number = "1",

}

Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus. / Gamboa, Maria C.; Rasmussen-Poblete, Susana; Valenzuela, Pablo D.T.; Krauskopf, Erwin.

En: Plant Physiology and Biochemistry, Vol. 45, N.º 1, 01.01.2007, p. 1-5.

Resultado de la investigación: Article

TY - JOUR

T1 - Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus

AU - Gamboa, Maria C.

AU - Rasmussen-Poblete, Susana

AU - Valenzuela, Pablo D.T.

AU - Krauskopf, Erwin

PY - 2007/1/1

Y1 - 2007/1/1

N2 - The transcription factors CBF/DREB play an important role during low temperature, drought and high-salt stress in higher plants. In this work, we isolated one full-length CBF cDNA clone from the angiosperm Eucalyptus globulus. The derived peptide sequence reveals that it encodes a transcriptional activator that has all the characteristic motifs present in CBF proteins previously described in Arabidopsis and tomato. RT-PCR analysis shows that EgCBF1 is transiently induced in E. globulus seedlings that had been exposed to low temperature within the first 15 min. These results suggest that the isolated CBF gene participates in the cold responsive pathway of E. globulus.

AB - The transcription factors CBF/DREB play an important role during low temperature, drought and high-salt stress in higher plants. In this work, we isolated one full-length CBF cDNA clone from the angiosperm Eucalyptus globulus. The derived peptide sequence reveals that it encodes a transcriptional activator that has all the characteristic motifs present in CBF proteins previously described in Arabidopsis and tomato. RT-PCR analysis shows that EgCBF1 is transiently induced in E. globulus seedlings that had been exposed to low temperature within the first 15 min. These results suggest that the isolated CBF gene participates in the cold responsive pathway of E. globulus.

KW - Abiotic stress

KW - Acclimation

KW - CBF

KW - Cold-tolerance

KW - Eucalyptus

KW - Forest biotechnology

UR - http://www.scopus.com/inward/record.url?scp=33847636665&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2006.12.006

DO - 10.1016/j.plaphy.2006.12.006

M3 - Article

C2 - 17303430

AN - SCOPUS:33847636665

VL - 45

SP - 1

EP - 5

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

IS - 1

ER -