Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases

P. Valenzuela, G. I. Bell, F. Weinberg, W. J. Rutter

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

This chapter describes the most recent and general procedures used in the purification of eukaryotic nuclear RNA polymerases,with special emphasis on those enzymes that have been extensively purified. Detailed methods are presented for the purification of yeast RNA polymerases that have been selected because of the convenience of their source, the authors' interest and experience, and the possible general applicability of this procedure for the purification of polymerases from other sources. Eukaryotic cells contain multiple RNA polymerases. The polymerase content in higher cells is quite low and unlike the bacterial polymerase the enzymes are difficult to extract as they occur tightly bound to chromosomal components, such as DNA, RNA, histones, and acidic proteins. Eukaryotic RNA polymerases are complex structures composed of multiple subunits, and losses of activity are usually encountered owing to the apparently delicate nature of these molecules.

Idioma originalEnglish
Páginas (desde-hasta)1-26
Número de páginas26
PublicaciónMethods in Cell Biology
Volumen19
N.ºC
DOI
EstadoPublished - 1 ene 1978

Huella dactilar

DNA-Directed RNA Polymerases
Nuclear RNA
Eukaryotic Cells
Enzymes
Histones
Yeasts
RNA
DNA
Proteins

ASJC Scopus subject areas

  • Cell Biology

Citar esto

Valenzuela, P. ; Bell, G. I. ; Weinberg, F. ; Rutter, W. J. / Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases. En: Methods in Cell Biology. 1978 ; Vol. 19, N.º C. pp. 1-26.
@article{8de4f4bc0ea84d3e9e832d892a13c6b9,
title = "Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases",
abstract = "This chapter describes the most recent and general procedures used in the purification of eukaryotic nuclear RNA polymerases,with special emphasis on those enzymes that have been extensively purified. Detailed methods are presented for the purification of yeast RNA polymerases that have been selected because of the convenience of their source, the authors' interest and experience, and the possible general applicability of this procedure for the purification of polymerases from other sources. Eukaryotic cells contain multiple RNA polymerases. The polymerase content in higher cells is quite low and unlike the bacterial polymerase the enzymes are difficult to extract as they occur tightly bound to chromosomal components, such as DNA, RNA, histones, and acidic proteins. Eukaryotic RNA polymerases are complex structures composed of multiple subunits, and losses of activity are usually encountered owing to the apparently delicate nature of these molecules.",
author = "P. Valenzuela and Bell, {G. I.} and F. Weinberg and Rutter, {W. J.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/S0091-679X(08)60006-0",
language = "English",
volume = "19",
pages = "1--26",
journal = "Methods in Cell Biology",
issn = "0091-679X",
publisher = "Academic Press Inc.",
number = "C",

}

Valenzuela, P, Bell, GI, Weinberg, F & Rutter, WJ 1978, 'Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases', Methods in Cell Biology, vol. 19, n.º C, pp. 1-26. https://doi.org/10.1016/S0091-679X(08)60006-0

Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases. / Valenzuela, P.; Bell, G. I.; Weinberg, F.; Rutter, W. J.

En: Methods in Cell Biology, Vol. 19, N.º C, 01.01.1978, p. 1-26.

Resultado de la investigación: Article

TY - JOUR

T1 - Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases

AU - Valenzuela, P.

AU - Bell, G. I.

AU - Weinberg, F.

AU - Rutter, W. J.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - This chapter describes the most recent and general procedures used in the purification of eukaryotic nuclear RNA polymerases,with special emphasis on those enzymes that have been extensively purified. Detailed methods are presented for the purification of yeast RNA polymerases that have been selected because of the convenience of their source, the authors' interest and experience, and the possible general applicability of this procedure for the purification of polymerases from other sources. Eukaryotic cells contain multiple RNA polymerases. The polymerase content in higher cells is quite low and unlike the bacterial polymerase the enzymes are difficult to extract as they occur tightly bound to chromosomal components, such as DNA, RNA, histones, and acidic proteins. Eukaryotic RNA polymerases are complex structures composed of multiple subunits, and losses of activity are usually encountered owing to the apparently delicate nature of these molecules.

AB - This chapter describes the most recent and general procedures used in the purification of eukaryotic nuclear RNA polymerases,with special emphasis on those enzymes that have been extensively purified. Detailed methods are presented for the purification of yeast RNA polymerases that have been selected because of the convenience of their source, the authors' interest and experience, and the possible general applicability of this procedure for the purification of polymerases from other sources. Eukaryotic cells contain multiple RNA polymerases. The polymerase content in higher cells is quite low and unlike the bacterial polymerase the enzymes are difficult to extract as they occur tightly bound to chromosomal components, such as DNA, RNA, histones, and acidic proteins. Eukaryotic RNA polymerases are complex structures composed of multiple subunits, and losses of activity are usually encountered owing to the apparently delicate nature of these molecules.

UR - http://www.scopus.com/inward/record.url?scp=0017831538&partnerID=8YFLogxK

U2 - 10.1016/S0091-679X(08)60006-0

DO - 10.1016/S0091-679X(08)60006-0

M3 - Article

C2 - 357912

AN - SCOPUS:0017831538

VL - 19

SP - 1

EP - 26

JO - Methods in Cell Biology

JF - Methods in Cell Biology

SN - 0091-679X

IS - C

ER -