Is chytridiomycosis driving darwin's frogs to extinction?

Claudio Soto-Azat, Andrés Valenzuela-Sánchez, Barry T. Clarke, Klaus Busse, Juan Carlos Ortiz, Carlos Barrientos, Andrew A. Cunningham

Resultado de la investigación: Article

20 Citas (Scopus)

Resumen

Darwin's frogs (Rhinoderma darwinii and R. rufum) are two species of mouth brooding frogs from Chile and Argentina that have experienced marked population declines. Rhinoderma rufum has not been found in the wild since 1980. We investigated historical and current evidence of Batrachochytrium dendrobatidis (Bd) infection in Rhinoderma spp. to determine whether chytridiomycosis is implicated in the population declines of these species. Archived and live specimens of Rhinoderma spp., sympatric amphibians and amphibians at sites where Rhinoderma sp. had recently gone extinct were examined for Bd infection using quantitative real-time PCR. Six (0.9%) of 662 archived anurans tested positive for Bd (4/289 R. darwinii; 1/266 R. rufum and 1/107 other anurans), all of which had been collected between 1970 and 1978. An overall Bd-infection prevalence of 12.5% was obtained from 797 swabs taken from 369 extant individuals of R. darwinii and 428 individuals representing 18 other species of anurans found at sites with current and recent presence of the two Rhinoderma species. In extant R. darwinii, Bd-infection prevalence (1.9%) was significantly lower than that found in other anurans (7.3%). The prevalence of infection (30%) in other amphibian species was significantly higher in sites where either Rhinoderma spp. had become extinct or was experiencing severe population declines than in sites where there had been no apparent decline (3.0%; x2 = 106.407, P<0.001). This is the first report of widespread Bd presence in Chile and our results are consistent with Rhinoderma spp. declines being due to Bd infection, although additional field and laboratory investigations are required to investigate this further.

Idioma originalEnglish
Número de artículoe79862
PublicaciónPLoS ONE
Volumen8
N.º11
DOI
EstadoPublished - 20 nov 2013

Huella dactilar

Chytridiomycota
Anura
frogs
extinction
infection
Amphibians
amphibians
Infection
Chile
Population
Argentina
mouth
quantitative polymerase chain reaction
Mouth
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Citar esto

Soto-Azat, C., Valenzuela-Sánchez, A., Clarke, B. T., Busse, K., Ortiz, J. C., Barrientos, C., & Cunningham, A. A. (2013). Is chytridiomycosis driving darwin's frogs to extinction? PLoS ONE, 8(11), [e79862]. https://doi.org/10.1371/journal.pone.0079862
Soto-Azat, Claudio ; Valenzuela-Sánchez, Andrés ; Clarke, Barry T. ; Busse, Klaus ; Ortiz, Juan Carlos ; Barrientos, Carlos ; Cunningham, Andrew A. / Is chytridiomycosis driving darwin's frogs to extinction?. En: PLoS ONE. 2013 ; Vol. 8, N.º 11.
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abstract = "Darwin's frogs (Rhinoderma darwinii and R. rufum) are two species of mouth brooding frogs from Chile and Argentina that have experienced marked population declines. Rhinoderma rufum has not been found in the wild since 1980. We investigated historical and current evidence of Batrachochytrium dendrobatidis (Bd) infection in Rhinoderma spp. to determine whether chytridiomycosis is implicated in the population declines of these species. Archived and live specimens of Rhinoderma spp., sympatric amphibians and amphibians at sites where Rhinoderma sp. had recently gone extinct were examined for Bd infection using quantitative real-time PCR. Six (0.9{\%}) of 662 archived anurans tested positive for Bd (4/289 R. darwinii; 1/266 R. rufum and 1/107 other anurans), all of which had been collected between 1970 and 1978. An overall Bd-infection prevalence of 12.5{\%} was obtained from 797 swabs taken from 369 extant individuals of R. darwinii and 428 individuals representing 18 other species of anurans found at sites with current and recent presence of the two Rhinoderma species. In extant R. darwinii, Bd-infection prevalence (1.9{\%}) was significantly lower than that found in other anurans (7.3{\%}). The prevalence of infection (30{\%}) in other amphibian species was significantly higher in sites where either Rhinoderma spp. had become extinct or was experiencing severe population declines than in sites where there had been no apparent decline (3.0{\%}; x2 = 106.407, P<0.001). This is the first report of widespread Bd presence in Chile and our results are consistent with Rhinoderma spp. declines being due to Bd infection, although additional field and laboratory investigations are required to investigate this further.",
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Soto-Azat, C, Valenzuela-Sánchez, A, Clarke, BT, Busse, K, Ortiz, JC, Barrientos, C & Cunningham, AA 2013, 'Is chytridiomycosis driving darwin's frogs to extinction?', PLoS ONE, vol. 8, n.º 11, e79862. https://doi.org/10.1371/journal.pone.0079862

Is chytridiomycosis driving darwin's frogs to extinction? / Soto-Azat, Claudio; Valenzuela-Sánchez, Andrés; Clarke, Barry T.; Busse, Klaus; Ortiz, Juan Carlos; Barrientos, Carlos; Cunningham, Andrew A.

En: PLoS ONE, Vol. 8, N.º 11, e79862, 20.11.2013.

Resultado de la investigación: Article

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Soto-Azat C, Valenzuela-Sánchez A, Clarke BT, Busse K, Ortiz JC, Barrientos C y otros. Is chytridiomycosis driving darwin's frogs to extinction? PLoS ONE. 2013 nov 20;8(11). e79862. https://doi.org/10.1371/journal.pone.0079862