Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

Pamela Ruiz, Miguel Balado, Alicia E. Toranzo, Matías Poblete-Morales, Manuel L. Lemos, Ruben Avendaño-Herrera

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2'-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.

Idioma originalEnglish
Páginas (desde-hasta)217-226
Número de páginas10
PublicaciónDiseases of Aquatic Organisms
Volumen118
N.º3
DOI
EstadoPublished - 30 mar 2016

Huella dactilar

Vibrio ordalii
siderophore
siderophores
Salmo salar
Chile
assimilation (physiology)
iron
hemoglobin
virulence
assay
heme
chelating agent
pathogenicity
salmonid
rainbow
aquaculture
bioassay
vibriosis
assimilation
assays

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Citar esto

Ruiz, Pamela ; Balado, Miguel ; Toranzo, Alicia E. ; Poblete-Morales, Matías ; Lemos, Manuel L. ; Avendaño-Herrera, Ruben. / Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile. En: Diseases of Aquatic Organisms. 2016 ; Vol. 118, N.º 3. pp. 217-226.
@article{b8c40a5f52674e1391ee697d917e1f10,
title = "Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile",
abstract = "Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2'-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.",
keywords = "Atlantic salmon, Iron sources, Siderophore, Vibrio ordalii, Virulence mechanisms",
author = "Pamela Ruiz and Miguel Balado and Toranzo, {Alicia E.} and Mat{\'i}as Poblete-Morales and Lemos, {Manuel L.} and Ruben Avenda{\~n}o-Herrera",
year = "2016",
month = "3",
day = "30",
doi = "10.3354/dao02976",
language = "English",
volume = "118",
pages = "217--226",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "3",

}

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile. / Ruiz, Pamela; Balado, Miguel; Toranzo, Alicia E.; Poblete-Morales, Matías; Lemos, Manuel L.; Avendaño-Herrera, Ruben.

En: Diseases of Aquatic Organisms, Vol. 118, N.º 3, 30.03.2016, p. 217-226.

Resultado de la investigación: Article

TY - JOUR

T1 - Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

AU - Ruiz, Pamela

AU - Balado, Miguel

AU - Toranzo, Alicia E.

AU - Poblete-Morales, Matías

AU - Lemos, Manuel L.

AU - Avendaño-Herrera, Ruben

PY - 2016/3/30

Y1 - 2016/3/30

N2 - Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2'-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.

AB - Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2'-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.

KW - Atlantic salmon

KW - Iron sources

KW - Siderophore

KW - Vibrio ordalii

KW - Virulence mechanisms

UR - http://www.scopus.com/inward/record.url?scp=84962440975&partnerID=8YFLogxK

U2 - 10.3354/dao02976

DO - 10.3354/dao02976

M3 - Article

C2 - 27025309

AN - SCOPUS:84962440975

VL - 118

SP - 217

EP - 226

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 3

ER -