In vitro modulation of Drimys winteri bark extract and the active compound polygodial on Salmo salar immune genes after exposure to Saprolegnia parasitica

D. Pereira-Torres, A. T. Gonçalves, V. Ulloa, R. Martínez, H. Carrasco, A. F. Olea, L. Espinoza, C. Gallardo-Escárate, A. Astuya

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

The rapid development of the aquaculture industry has global concerns with health management and control strategies to prevent and/or treat diseases and increase sustainability standards. Saprolegniosis is a disease caused by Saprolegnia parasitica, and is characterized by promoting an immunosuppression in the host. This study evaluated in vitro the extract and one active compound (polygodial) of Drimys winteri, a Chilean medicinal tree as a potential early immunostimulatory aid in Saprolegniosis control. Atlantic salmon (Salmo salar) head kidney cells (ASK-1) were incubated with both extract and pure polygodial before exposure to S. parasitica mycelium, and the expression of the immune-related genes interleukin 1β (IL-1β), interferon α (IFNα), and major histocompatibility complex II (MHCII) was evaluated. Both evidenced immunomodulatory capacities by increasing gene expressions. This immunomodulation related to a mitigatory action counteracting the immunosuppressing effects of S. parasitica. Despite that most immune-related genes were up-regulated, the down-regulation of MHCII, characteristic of S. parasitica infection, was lessened by pre-incubation with the compounds. This study provides the first insight on the potential of D. winteri bark extract as a possible immunomodulatory and defensive strategy against this oomycete infection in fish.

Idioma originalEnglish
Páginas (desde-hasta)103-108
Número de páginas6
PublicaciónFish and Shellfish Immunology
Volumen59
DOI
EstadoPublished - 1 dic 2016

Huella dactilar

Drimys winteri
major histocompatibility complex
saprolegniosis
Salmo salar
bark
Genes
Modulation
Aquaculture
aquaculture industry
gene
extracts
Saprolegnia parasitica
Interleukin-1
Gene expression
Fish
Interferons
gene expression
Sustainable development
aid
immunomodulators

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Citar esto

Pereira-Torres, D. ; Gonçalves, A. T. ; Ulloa, V. ; Martínez, R. ; Carrasco, H. ; Olea, A. F. ; Espinoza, L. ; Gallardo-Escárate, C. ; Astuya, A. / In vitro modulation of Drimys winteri bark extract and the active compound polygodial on Salmo salar immune genes after exposure to Saprolegnia parasitica. En: Fish and Shellfish Immunology. 2016 ; Vol. 59. pp. 103-108.
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abstract = "The rapid development of the aquaculture industry has global concerns with health management and control strategies to prevent and/or treat diseases and increase sustainability standards. Saprolegniosis is a disease caused by Saprolegnia parasitica, and is characterized by promoting an immunosuppression in the host. This study evaluated in vitro the extract and one active compound (polygodial) of Drimys winteri, a Chilean medicinal tree as a potential early immunostimulatory aid in Saprolegniosis control. Atlantic salmon (Salmo salar) head kidney cells (ASK-1) were incubated with both extract and pure polygodial before exposure to S. parasitica mycelium, and the expression of the immune-related genes interleukin 1β (IL-1β), interferon α (IFNα), and major histocompatibility complex II (MHCII) was evaluated. Both evidenced immunomodulatory capacities by increasing gene expressions. This immunomodulation related to a mitigatory action counteracting the immunosuppressing effects of S. parasitica. Despite that most immune-related genes were up-regulated, the down-regulation of MHCII, characteristic of S. parasitica infection, was lessened by pre-incubation with the compounds. This study provides the first insight on the potential of D. winteri bark extract as a possible immunomodulatory and defensive strategy against this oomycete infection in fish.",
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In vitro modulation of Drimys winteri bark extract and the active compound polygodial on Salmo salar immune genes after exposure to Saprolegnia parasitica. / Pereira-Torres, D.; Gonçalves, A. T.; Ulloa, V.; Martínez, R.; Carrasco, H.; Olea, A. F.; Espinoza, L.; Gallardo-Escárate, C.; Astuya, A.

En: Fish and Shellfish Immunology, Vol. 59, 01.12.2016, p. 103-108.

Resultado de la investigación: Article

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T1 - In vitro modulation of Drimys winteri bark extract and the active compound polygodial on Salmo salar immune genes after exposure to Saprolegnia parasitica

AU - Pereira-Torres, D.

AU - Gonçalves, A. T.

AU - Ulloa, V.

AU - Martínez, R.

AU - Carrasco, H.

AU - Olea, A. F.

AU - Espinoza, L.

AU - Gallardo-Escárate, C.

AU - Astuya, A.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The rapid development of the aquaculture industry has global concerns with health management and control strategies to prevent and/or treat diseases and increase sustainability standards. Saprolegniosis is a disease caused by Saprolegnia parasitica, and is characterized by promoting an immunosuppression in the host. This study evaluated in vitro the extract and one active compound (polygodial) of Drimys winteri, a Chilean medicinal tree as a potential early immunostimulatory aid in Saprolegniosis control. Atlantic salmon (Salmo salar) head kidney cells (ASK-1) were incubated with both extract and pure polygodial before exposure to S. parasitica mycelium, and the expression of the immune-related genes interleukin 1β (IL-1β), interferon α (IFNα), and major histocompatibility complex II (MHCII) was evaluated. Both evidenced immunomodulatory capacities by increasing gene expressions. This immunomodulation related to a mitigatory action counteracting the immunosuppressing effects of S. parasitica. Despite that most immune-related genes were up-regulated, the down-regulation of MHCII, characteristic of S. parasitica infection, was lessened by pre-incubation with the compounds. This study provides the first insight on the potential of D. winteri bark extract as a possible immunomodulatory and defensive strategy against this oomycete infection in fish.

AB - The rapid development of the aquaculture industry has global concerns with health management and control strategies to prevent and/or treat diseases and increase sustainability standards. Saprolegniosis is a disease caused by Saprolegnia parasitica, and is characterized by promoting an immunosuppression in the host. This study evaluated in vitro the extract and one active compound (polygodial) of Drimys winteri, a Chilean medicinal tree as a potential early immunostimulatory aid in Saprolegniosis control. Atlantic salmon (Salmo salar) head kidney cells (ASK-1) were incubated with both extract and pure polygodial before exposure to S. parasitica mycelium, and the expression of the immune-related genes interleukin 1β (IL-1β), interferon α (IFNα), and major histocompatibility complex II (MHCII) was evaluated. Both evidenced immunomodulatory capacities by increasing gene expressions. This immunomodulation related to a mitigatory action counteracting the immunosuppressing effects of S. parasitica. Despite that most immune-related genes were up-regulated, the down-regulation of MHCII, characteristic of S. parasitica infection, was lessened by pre-incubation with the compounds. This study provides the first insight on the potential of D. winteri bark extract as a possible immunomodulatory and defensive strategy against this oomycete infection in fish.

KW - D. winteri

KW - Gene expression

KW - Immunostimulant

KW - In vitro

KW - Polygodial

KW - S. parasitica

KW - Saprolegniosis

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DO - 10.1016/j.fsi.2016.10.035

M3 - Article

VL - 59

SP - 103

EP - 108

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -