In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis

Marcos Cortés, Patricio Sánchez, Pamela Ruiz, Ronie Haro, Jerson Sáez, Fabián Sánchez, Mauricio Hernández, Cristian Oliver, Alejandro J. Yáñez

Resultado de la investigación: Contribución a una revistaArtículo

3 Citas (Scopus)

Resumen

Piscirickettsia salmonis is an intracellular bacterium and the causative agent of Piscirickettsiosis, a disease responsible for considerable mortalities in the Chilean salmon farming industry. Currently, P. salmonis protein translocation across the membrane and the mechanisms by which virulence factors are delivered to host cells are poorly understood. However, it is known that Gram-negative bacteria possess several mechanisms that transport proteins to the periplasmic and extracellular compartments. The aim of this study was to evaluate the expressional changes of several genes in the P. salmonis Sec-dependent pathway and type 4B secretion system during in vitro infection. Genes homologous and the main proteins belonging to Sec-dependent pathway and Type 4 Dot/Icm secretion system were found in the genome and proteome of P. salmonis AUSTRAL-005 strain. Additionally, several genes of these protein transport mechanisms were overexpressed during in vitro P. salmonis infection in SHK-1 cell line. The obtained data indicate that the Sec-dependent pathway and Type 4B secretion system are biologically active during P. salmonis infection. These mechanisms could contribute to the recycling of proteins into the inner and outer bacterial membrane and in translocate virulence factors to infected cell, which would favor the structural integrity and virulence of this bacterium.

Idioma originalInglés
Páginas (desde-hasta)586-593
Número de páginas8
PublicaciónMicrobial Pathogenesis
Volumen110
DOI
EstadoPublicada - 1 sep 2017

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Enfermedades infecciosas

Huella Profundice en los temas de investigación de 'In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis'. En conjunto forman una huella única.

  • Citar esto

    Cortés, M., Sánchez, P., Ruiz, P., Haro, R., Sáez, J., Sánchez, F., Hernández, M., Oliver, C., & Yáñez, A. J. (2017). In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis. Microbial Pathogenesis, 110, 586-593. https://doi.org/10.1016/j.micpath.2017.08.003