Intraspecific variability among Chilean strains of the astaxanthin-producing microalga Haematococcus pluvialis (Chlorophyta): An opportunity for its genetic improvement by simple selection

Patricia I. Gómez, Paola Haro, Priscilla Lagos, Yussi Palacios, Jennifer Torres, Katia Sáez, Pablo Castro, Víctor González, Ingrid Inostroza, Mariela A. González

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

The unicellular green alga Haematococcus pluvialis accumulates large quantities of astaxanthin, a highly valuable carotenoid pigment in the aquaculture, food, and pharmaceutical industries. Biological diversity within a species with a biotechnological interest allows applying strain selection procedures avoiding genetic manipulation. The aim of this study was to determine growth and carotenogenic capacity among 13 strains isolated from a latitudinal range throughout south-central and southern Chile, in order to know their biological diversity and biotechnological potential. Strains were isolated from rain pools located in public squares and cemeteries of the cities: Concepción, Valdivia, Osorno, Puerto Varas, and Castro. All the Chilean strains were more carotenogenic than the reference strain Steptoe. Physiological attributes differed greatly among the strains. Principal component analysis (PCA) of growth and carotenogenesis parameters grouped the strains into four groups, two of them composed by only one strain (CCM-UdeC-038 and CCM-UdeC- 039). These latter strains were also the most carotenogenic ones, being strain CCM-UdeC-039 the one that accumulated the highest amount of astaxanthin (7 mg astaxanthin L−1 culture) associated to the highest total carotenoid content (1.1 % by dry biomass) and carotenoids/chlorophyll ratio (2.6). These results demonstrate that a great physiological diversity exists at intraspecific level in H. pluvialis (even among strains coming from the same geographical origin), which allows performing genetic improvement by means of simple selection of new strains from nature. Further research will be focused on finding out the culture conditions that optimize the astaxanthin production of the most promising strains.

Idioma originalEnglish
Páginas (desde-hasta)2115-2122
Número de páginas8
PublicaciónJournal of Applied Phycology
Volumen28
N.º4
DOI
EstadoPublished - 1 ago 2016
Publicado de forma externa

Huella dactilar

Haematococcus pluvialis
microalga
astaxanthin
carotenoid
genetic improvement
Chlorophyta
cemetery
pharmaceutical industry
aquaculture industry
food industry
green alga
pigment
principal component analysis
chlorophyll
carotenoids
biomass
intraspecific variation
biodiversity
genetic engineering

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Citar esto

Gómez, Patricia I. ; Haro, Paola ; Lagos, Priscilla ; Palacios, Yussi ; Torres, Jennifer ; Sáez, Katia ; Castro, Pablo ; González, Víctor ; Inostroza, Ingrid ; González, Mariela A. / Intraspecific variability among Chilean strains of the astaxanthin-producing microalga Haematococcus pluvialis (Chlorophyta) : An opportunity for its genetic improvement by simple selection. En: Journal of Applied Phycology. 2016 ; Vol. 28, N.º 4. pp. 2115-2122.
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abstract = "The unicellular green alga Haematococcus pluvialis accumulates large quantities of astaxanthin, a highly valuable carotenoid pigment in the aquaculture, food, and pharmaceutical industries. Biological diversity within a species with a biotechnological interest allows applying strain selection procedures avoiding genetic manipulation. The aim of this study was to determine growth and carotenogenic capacity among 13 strains isolated from a latitudinal range throughout south-central and southern Chile, in order to know their biological diversity and biotechnological potential. Strains were isolated from rain pools located in public squares and cemeteries of the cities: Concepci{\'o}n, Valdivia, Osorno, Puerto Varas, and Castro. All the Chilean strains were more carotenogenic than the reference strain Steptoe. Physiological attributes differed greatly among the strains. Principal component analysis (PCA) of growth and carotenogenesis parameters grouped the strains into four groups, two of them composed by only one strain (CCM-UdeC-038 and CCM-UdeC- 039). These latter strains were also the most carotenogenic ones, being strain CCM-UdeC-039 the one that accumulated the highest amount of astaxanthin (7 mg astaxanthin L−1 culture) associated to the highest total carotenoid content (1.1 {\%} by dry biomass) and carotenoids/chlorophyll ratio (2.6). These results demonstrate that a great physiological diversity exists at intraspecific level in H. pluvialis (even among strains coming from the same geographical origin), which allows performing genetic improvement by means of simple selection of new strains from nature. Further research will be focused on finding out the culture conditions that optimize the astaxanthin production of the most promising strains.",
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Intraspecific variability among Chilean strains of the astaxanthin-producing microalga Haematococcus pluvialis (Chlorophyta) : An opportunity for its genetic improvement by simple selection. / Gómez, Patricia I.; Haro, Paola; Lagos, Priscilla; Palacios, Yussi; Torres, Jennifer; Sáez, Katia; Castro, Pablo; González, Víctor; Inostroza, Ingrid; González, Mariela A.

En: Journal of Applied Phycology, Vol. 28, N.º 4, 01.08.2016, p. 2115-2122.

Resultado de la investigación: Article

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T1 - Intraspecific variability among Chilean strains of the astaxanthin-producing microalga Haematococcus pluvialis (Chlorophyta)

T2 - An opportunity for its genetic improvement by simple selection

AU - Gómez, Patricia I.

AU - Haro, Paola

AU - Lagos, Priscilla

AU - Palacios, Yussi

AU - Torres, Jennifer

AU - Sáez, Katia

AU - Castro, Pablo

AU - González, Víctor

AU - Inostroza, Ingrid

AU - González, Mariela A.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - The unicellular green alga Haematococcus pluvialis accumulates large quantities of astaxanthin, a highly valuable carotenoid pigment in the aquaculture, food, and pharmaceutical industries. Biological diversity within a species with a biotechnological interest allows applying strain selection procedures avoiding genetic manipulation. The aim of this study was to determine growth and carotenogenic capacity among 13 strains isolated from a latitudinal range throughout south-central and southern Chile, in order to know their biological diversity and biotechnological potential. Strains were isolated from rain pools located in public squares and cemeteries of the cities: Concepción, Valdivia, Osorno, Puerto Varas, and Castro. All the Chilean strains were more carotenogenic than the reference strain Steptoe. Physiological attributes differed greatly among the strains. Principal component analysis (PCA) of growth and carotenogenesis parameters grouped the strains into four groups, two of them composed by only one strain (CCM-UdeC-038 and CCM-UdeC- 039). These latter strains were also the most carotenogenic ones, being strain CCM-UdeC-039 the one that accumulated the highest amount of astaxanthin (7 mg astaxanthin L−1 culture) associated to the highest total carotenoid content (1.1 % by dry biomass) and carotenoids/chlorophyll ratio (2.6). These results demonstrate that a great physiological diversity exists at intraspecific level in H. pluvialis (even among strains coming from the same geographical origin), which allows performing genetic improvement by means of simple selection of new strains from nature. Further research will be focused on finding out the culture conditions that optimize the astaxanthin production of the most promising strains.

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