Intramuscular Hamstring Stiffness Affects Anatomically Modeled Localized Muscle Strain during Passive Hip Flexion

Rehbein, CO, McDougle, JM, Peñailillo, L, and Earp, JE. Intramuscular hamstring stiffness affects anatomically modeled localized muscle strain during passive hip flexion. J Strength Cond Res XX(X): 000-000, 2024 - Hamstring strain injuries occur when localized tissue strain capacity is exceeded. Localized strain may be affected by intramuscular variation in stiffness, but research in this area is lacking. The purpose of this study was to determine the effects of intramuscular hamstring stiffness on localized muscle strain during passive hip flexion. Twenty-eight (age 25.0 ± 4.9 years) healthy women (n = 15) and men (n = 13) had biceps femoris, semitendinosus, and semimembranosus stiffness measured proximally, medially, and distally during passive hip flexion and extension using shear-wave elastography. Anthropometric and stiffness measurements were entered into an anatomical model of equivalent springs to estimate localized tissue strain and differentiate between the relative contribution to passive strain from each muscular region. In shortened and stretched positions, stiffness was lowest proximally for all muscles (Cohen's d = 0.66-0.79, p < 0.001). In addition, relative strain contribution was greater proximally (37.5-39.4%) compared with middle (31.74-32.2%) or distal (28.6-30.3%) regions (p < 0.001), with proximal contribution to strain increasing with greater hip flexion. Our results suggest that intramuscular variations in passive hamstring stiffness contribute to inhomogeneous strain throughout the muscle during passive hip flexion. Given the prevalence of proximal stretch-pattern strain injuries, variation in intramuscular stiffness may contribute to risk for such injuries.