TY - JOUR
T1 - Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells
AU - Concha, Ilona I.
AU - Figueroa, Jaime
AU - Concha, Margarita I.
AU - Ueda, Kunihiro
AU - Burzio, Luis O.
N1 - Funding Information:
This researchw as supportedb y Grant RS-85-10f rom the ResearchF und of the UniversidadA ustral de Chile; Grant I/61 457f rom the StiftungV olkswagenwerk,F RG; Grants 186-87a nd 0942-88fr om the FONDECYT, Chile (LOB); and grants-in-aidf or scientific research and cancer research from the Ministry of Education, Science, and Culture, Japan (K.U.).
PY - 1989/2
Y1 - 1989/2
N2 - The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemical staining with the enzyme-specific antibody. The synthetase was localized in the nuclei of interstitial cells, Sertoli cells, spermatogonia, and spermatocytes. In addition, round spermatids showed a granular staining in the cytoplasm, which was comparable in intensity with that in the nucleus. The cytoplasmic synthetase had a molecular weight of 115,000 and synthesized oligomers of ADP-ribose on itself (automodification). The synthetase activity in the isolated cytoplasmic fraction was stimulated about threefold by the addition of DNA and depressed by treatment with DNase I, suggesting the presence of endogenous activator DNA. A candidate DNA for such an activator was isolated from the microsomal-ribosomal fraction, and identified tentatively as mitochondrial DNA on the basis of its size and restriction fragment patterns.
AB - The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemical staining with the enzyme-specific antibody. The synthetase was localized in the nuclei of interstitial cells, Sertoli cells, spermatogonia, and spermatocytes. In addition, round spermatids showed a granular staining in the cytoplasm, which was comparable in intensity with that in the nucleus. The cytoplasmic synthetase had a molecular weight of 115,000 and synthesized oligomers of ADP-ribose on itself (automodification). The synthetase activity in the isolated cytoplasmic fraction was stimulated about threefold by the addition of DNA and depressed by treatment with DNase I, suggesting the presence of endogenous activator DNA. A candidate DNA for such an activator was isolated from the microsomal-ribosomal fraction, and identified tentatively as mitochondrial DNA on the basis of its size and restriction fragment patterns.
UR - http://www.scopus.com/inward/record.url?scp=0024548604&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(89)90063-3
DO - 10.1016/0014-4827(89)90063-3
M3 - Article
C2 - 2492470
AN - SCOPUS:0024548604
VL - 180
SP - 353
EP - 366
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -