Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells

D. Sauma, P. Michea, A. M. Lennon-Duménil, A. Fierro, J. Morales, M. Rosemblatt, M. R. Bono

Resultado de la investigación: Article

14 Citas (Scopus)

Resumen

Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 (IL-2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL-12, a cytokine related to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. This inhibition, which depends on the presence of IL-4 during LPS activation, is DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL-4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL-4 on DCs.

Idioma originalEnglish
Páginas (desde-hasta)183-189
Número de páginas7
PublicaciónScandinavian Journal of Immunology
Volumen59
N.º2
DOI
EstadoPublished - feb 2004

Huella dactilar

Interleukin-4
Dendritic Cells
Interleukin-2
Lipopolysaccharides
Interleukin-12
Granulocyte-Macrophage Colony-Stimulating Factor
T-Lymphocytes
Bone Marrow Cells
Adaptive Immunity
Cell Differentiation
Bone Marrow
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Citar esto

Sauma, D. ; Michea, P. ; Lennon-Duménil, A. M. ; Fierro, A. ; Morales, J. ; Rosemblatt, M. ; Bono, M. R. / Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells. En: Scandinavian Journal of Immunology. 2004 ; Vol. 59, N.º 2. pp. 183-189.
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Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells. / Sauma, D.; Michea, P.; Lennon-Duménil, A. M.; Fierro, A.; Morales, J.; Rosemblatt, M.; Bono, M. R.

En: Scandinavian Journal of Immunology, Vol. 59, N.º 2, 02.2004, p. 183-189.

Resultado de la investigación: Article

TY - JOUR

T1 - Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells

AU - Sauma, D.

AU - Michea, P.

AU - Lennon-Duménil, A. M.

AU - Fierro, A.

AU - Morales, J.

AU - Rosemblatt, M.

AU - Bono, M. R.

PY - 2004/2

Y1 - 2004/2

N2 - Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 (IL-2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL-12, a cytokine related to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. This inhibition, which depends on the presence of IL-4 during LPS activation, is DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL-4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL-4 on DCs.

AB - Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 (IL-2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL-12, a cytokine related to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. This inhibition, which depends on the presence of IL-4 during LPS activation, is DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL-4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL-4 on DCs.

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