Interactions between Clostridium perfringens spores and Raw 264.7 macrophages

Daniel Paredes-Sabja, Mahfuzur R. Sarker

Resultado de la investigación: Article

18 Citas (Scopus)

Resumen

Clostridium perfringens is the causative agent of a variety of histotoxic infections in humans and animals. Studies on the early events of C. perfringens infections have been largely focused on the interactions between their vegetative cells and macrophages. Consequently, in the current study we have examined the interactions between C. perfringens spores and Raw 264.7 macrophages. Raw 264.7 cells were able to interact and phagocytose Clostridium perfringens spores of a food poisoning isolate, strain SM101, and a non-food borne isolate, strain F4969, albeit to different extents. Phagocytosis and to a lesser extent, association, of C. perfringens spores by Raw 2647 macrophages was completely inhibited in presence of cytochalasin D. Complement increased association and phagocytosis of C. perfringens spores by Raw 264.7 macrophages. Survival of C. perfringens spores during macrophage infection seems to depend on the ability of spore germination during infection as: (i) F4969 spores germinated during infection with Raw 264.7 macrophages and subsequently killed by macrophages; and (ii) SM101 spores remained dormant inside Raw 264.7 macrophages and thus survived up to 24h of infection. The invitro spore-resistance factors, α/β-type SASP, SpmA/B proteins and spore's core water content, seems to play no role in mediating SM101 spore-resistance to macrophages. Collectively, these results might well have implications in understanding the initial stages of infections by C. perfringens spores.

Idioma originalEnglish
Páginas (desde-hasta)148-156
Número de páginas9
PublicaciónAnaerobe
Volumen18
N.º1
DOI
EstadoPublished - 1 feb 2012

Huella dactilar

Clostridium perfringens
Spores
Macrophages
Infection
Phagocytosis
Clostridium Infections
Cytochalasin D
Foodborne Diseases
R Factors
Germination

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Citar esto

@article{e02635ae4fb143cf87409f0748689ca2,
title = "Interactions between Clostridium perfringens spores and Raw 264.7 macrophages",
abstract = "Clostridium perfringens is the causative agent of a variety of histotoxic infections in humans and animals. Studies on the early events of C. perfringens infections have been largely focused on the interactions between their vegetative cells and macrophages. Consequently, in the current study we have examined the interactions between C. perfringens spores and Raw 264.7 macrophages. Raw 264.7 cells were able to interact and phagocytose Clostridium perfringens spores of a food poisoning isolate, strain SM101, and a non-food borne isolate, strain F4969, albeit to different extents. Phagocytosis and to a lesser extent, association, of C. perfringens spores by Raw 2647 macrophages was completely inhibited in presence of cytochalasin D. Complement increased association and phagocytosis of C. perfringens spores by Raw 264.7 macrophages. Survival of C. perfringens spores during macrophage infection seems to depend on the ability of spore germination during infection as: (i) F4969 spores germinated during infection with Raw 264.7 macrophages and subsequently killed by macrophages; and (ii) SM101 spores remained dormant inside Raw 264.7 macrophages and thus survived up to 24h of infection. The invitro spore-resistance factors, α/β-type SASP, SpmA/B proteins and spore's core water content, seems to play no role in mediating SM101 spore-resistance to macrophages. Collectively, these results might well have implications in understanding the initial stages of infections by C. perfringens spores.",
keywords = "Clostridium perfringens, Germination, Macrophage, Spores",
author = "Daniel Paredes-Sabja and Sarker, {Mahfuzur R.}",
year = "2012",
month = "2",
day = "1",
doi = "10.1016/j.anaerobe.2011.12.019",
language = "English",
volume = "18",
pages = "148--156",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press Inc.",
number = "1",

}

Interactions between Clostridium perfringens spores and Raw 264.7 macrophages. / Paredes-Sabja, Daniel; Sarker, Mahfuzur R.

En: Anaerobe, Vol. 18, N.º 1, 01.02.2012, p. 148-156.

Resultado de la investigación: Article

TY - JOUR

T1 - Interactions between Clostridium perfringens spores and Raw 264.7 macrophages

AU - Paredes-Sabja, Daniel

AU - Sarker, Mahfuzur R.

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Clostridium perfringens is the causative agent of a variety of histotoxic infections in humans and animals. Studies on the early events of C. perfringens infections have been largely focused on the interactions between their vegetative cells and macrophages. Consequently, in the current study we have examined the interactions between C. perfringens spores and Raw 264.7 macrophages. Raw 264.7 cells were able to interact and phagocytose Clostridium perfringens spores of a food poisoning isolate, strain SM101, and a non-food borne isolate, strain F4969, albeit to different extents. Phagocytosis and to a lesser extent, association, of C. perfringens spores by Raw 2647 macrophages was completely inhibited in presence of cytochalasin D. Complement increased association and phagocytosis of C. perfringens spores by Raw 264.7 macrophages. Survival of C. perfringens spores during macrophage infection seems to depend on the ability of spore germination during infection as: (i) F4969 spores germinated during infection with Raw 264.7 macrophages and subsequently killed by macrophages; and (ii) SM101 spores remained dormant inside Raw 264.7 macrophages and thus survived up to 24h of infection. The invitro spore-resistance factors, α/β-type SASP, SpmA/B proteins and spore's core water content, seems to play no role in mediating SM101 spore-resistance to macrophages. Collectively, these results might well have implications in understanding the initial stages of infections by C. perfringens spores.

AB - Clostridium perfringens is the causative agent of a variety of histotoxic infections in humans and animals. Studies on the early events of C. perfringens infections have been largely focused on the interactions between their vegetative cells and macrophages. Consequently, in the current study we have examined the interactions between C. perfringens spores and Raw 264.7 macrophages. Raw 264.7 cells were able to interact and phagocytose Clostridium perfringens spores of a food poisoning isolate, strain SM101, and a non-food borne isolate, strain F4969, albeit to different extents. Phagocytosis and to a lesser extent, association, of C. perfringens spores by Raw 2647 macrophages was completely inhibited in presence of cytochalasin D. Complement increased association and phagocytosis of C. perfringens spores by Raw 264.7 macrophages. Survival of C. perfringens spores during macrophage infection seems to depend on the ability of spore germination during infection as: (i) F4969 spores germinated during infection with Raw 264.7 macrophages and subsequently killed by macrophages; and (ii) SM101 spores remained dormant inside Raw 264.7 macrophages and thus survived up to 24h of infection. The invitro spore-resistance factors, α/β-type SASP, SpmA/B proteins and spore's core water content, seems to play no role in mediating SM101 spore-resistance to macrophages. Collectively, these results might well have implications in understanding the initial stages of infections by C. perfringens spores.

KW - Clostridium perfringens

KW - Germination

KW - Macrophage

KW - Spores

UR - http://www.scopus.com/inward/record.url?scp=84856736130&partnerID=8YFLogxK

U2 - 10.1016/j.anaerobe.2011.12.019

DO - 10.1016/j.anaerobe.2011.12.019

M3 - Article

C2 - 22209938

AN - SCOPUS:84856736130

VL - 18

SP - 148

EP - 156

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

IS - 1

ER -