Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment

Virgílio Gavicho Uarrota, Claudia Fuentealba, Ignacia Hernández, Bruno Defilippi-Bruzzone, Claudio Meneses, Reinaldo Campos-Vargas, Susan Lurie, Maarten Hertog, Sebastien Carpentier, Carlos Poblete-Echeverría, Romina Pedreschi

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Ripening heterogeneity of Hass avocados results in inconsistent quality fruit delivered to the triggered and ready to eat markets. This research aimed to understand the effect of a heat shock (HS) prior to controlled atmosphere (CA) storage on the reduction of ripening heterogeneity. HS prior to CA storage reduces more drastically the ripening heterogeneity in middle season fruit. Via correlation network analysis we show the different metabolomics networks between HS and CA. High throughput proteomics revealed 135 differentially expressed proteins unique to middle season fruit triggered by HS. Further integration of metabolomics and proteomics data revealed that HS reduced the glycolytic throughput and induced protein degradation to deliver energy for the alternative ripening pathways. L-isoleucine, L-valine, L-aspartic and ubiquitin carboxyl-terminal hydrolase involved in protein degradation were positively correlated to HS samples. Our study provides new insights into the effectiveness of HS in synchronizing ripening of Hass avocados.

Idioma originalEnglish
Páginas (desde-hasta)512-521
Número de páginas10
PublicaciónFood Chemistry
Volumen289
DOI
EstadoPublished - 15 ago 2019

Huella dactilar

Persea
Metabolomics
avocados
metabolomics
Proteomics
proteomics
heat stress
Shock
Hot Temperature
Heat treatment
heat treatment
ripening
Fruits
Atmosphere
Fruit
controlled atmosphere storage
Protective atmospheres
protein degradation
Proteolysis
Throughput

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Citar esto

Gavicho Uarrota, Virgílio ; Fuentealba, Claudia ; Hernández, Ignacia ; Defilippi-Bruzzone, Bruno ; Meneses, Claudio ; Campos-Vargas, Reinaldo ; Lurie, Susan ; Hertog, Maarten ; Carpentier, Sebastien ; Poblete-Echeverría, Carlos ; Pedreschi, Romina. / Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment. En: Food Chemistry. 2019 ; Vol. 289. pp. 512-521.
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title = "Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment",
abstract = "Ripening heterogeneity of Hass avocados results in inconsistent quality fruit delivered to the triggered and ready to eat markets. This research aimed to understand the effect of a heat shock (HS) prior to controlled atmosphere (CA) storage on the reduction of ripening heterogeneity. HS prior to CA storage reduces more drastically the ripening heterogeneity in middle season fruit. Via correlation network analysis we show the different metabolomics networks between HS and CA. High throughput proteomics revealed 135 differentially expressed proteins unique to middle season fruit triggered by HS. Further integration of metabolomics and proteomics data revealed that HS reduced the glycolytic throughput and induced protein degradation to deliver energy for the alternative ripening pathways. L-isoleucine, L-valine, L-aspartic and ubiquitin carboxyl-terminal hydrolase involved in protein degradation were positively correlated to HS samples. Our study provides new insights into the effectiveness of HS in synchronizing ripening of Hass avocados.",
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Gavicho Uarrota, V, Fuentealba, C, Hernández, I, Defilippi-Bruzzone, B, Meneses, C, Campos-Vargas, R, Lurie, S, Hertog, M, Carpentier, S, Poblete-Echeverría, C & Pedreschi, R 2019, 'Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment', Food Chemistry, vol. 289, pp. 512-521. https://doi.org/10.1016/j.foodchem.2019.03.090

Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment. / Gavicho Uarrota, Virgílio; Fuentealba, Claudia; Hernández, Ignacia; Defilippi-Bruzzone, Bruno; Meneses, Claudio; Campos-Vargas, Reinaldo; Lurie, Susan; Hertog, Maarten; Carpentier, Sebastien; Poblete-Echeverría, Carlos; Pedreschi, Romina.

En: Food Chemistry, Vol. 289, 15.08.2019, p. 512-521.

Resultado de la investigación: Article

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T1 - Integration of proteomics and metabolomics data of early and middle season Hass avocados under heat treatment

AU - Gavicho Uarrota, Virgílio

AU - Fuentealba, Claudia

AU - Hernández, Ignacia

AU - Defilippi-Bruzzone, Bruno

AU - Meneses, Claudio

AU - Campos-Vargas, Reinaldo

AU - Lurie, Susan

AU - Hertog, Maarten

AU - Carpentier, Sebastien

AU - Poblete-Echeverría, Carlos

AU - Pedreschi, Romina

PY - 2019/8/15

Y1 - 2019/8/15

N2 - Ripening heterogeneity of Hass avocados results in inconsistent quality fruit delivered to the triggered and ready to eat markets. This research aimed to understand the effect of a heat shock (HS) prior to controlled atmosphere (CA) storage on the reduction of ripening heterogeneity. HS prior to CA storage reduces more drastically the ripening heterogeneity in middle season fruit. Via correlation network analysis we show the different metabolomics networks between HS and CA. High throughput proteomics revealed 135 differentially expressed proteins unique to middle season fruit triggered by HS. Further integration of metabolomics and proteomics data revealed that HS reduced the glycolytic throughput and induced protein degradation to deliver energy for the alternative ripening pathways. L-isoleucine, L-valine, L-aspartic and ubiquitin carboxyl-terminal hydrolase involved in protein degradation were positively correlated to HS samples. Our study provides new insights into the effectiveness of HS in synchronizing ripening of Hass avocados.

AB - Ripening heterogeneity of Hass avocados results in inconsistent quality fruit delivered to the triggered and ready to eat markets. This research aimed to understand the effect of a heat shock (HS) prior to controlled atmosphere (CA) storage on the reduction of ripening heterogeneity. HS prior to CA storage reduces more drastically the ripening heterogeneity in middle season fruit. Via correlation network analysis we show the different metabolomics networks between HS and CA. High throughput proteomics revealed 135 differentially expressed proteins unique to middle season fruit triggered by HS. Further integration of metabolomics and proteomics data revealed that HS reduced the glycolytic throughput and induced protein degradation to deliver energy for the alternative ripening pathways. L-isoleucine, L-valine, L-aspartic and ubiquitin carboxyl-terminal hydrolase involved in protein degradation were positively correlated to HS samples. Our study provides new insights into the effectiveness of HS in synchronizing ripening of Hass avocados.

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KW - GC-MS metabolomics

KW - Gel-free proteomics

KW - Heterogeneity

KW - L-alanine (PubChem CID:5950)

KW - L-aspartic acid (PubChem CID:5960)

KW - Palmitic acid (PubChem CID:985)

KW - Persea americana

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