Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet

Alejandra Espinosa, Cristian Campos, Alexis Díaz-Vegas, José E. Galgani, Nevenka Juretic, César Osorio-Fuentealba, José L. Bucarey, Gladys Tapia, Rodrigo Valenzuela, Ariel Contreras-Ferrat, Paola Llanos, Enrique Jaimovich

Resultado de la investigación: Article

20 Citas (Scopus)

Resumen

Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) produced by NADPH oxidase 2 (NOX2) is involved in signaling pathways triggered by insulin. We evaluated oxidative status in skeletal muscle fibers from insulin-resistant and control mice by determining H2O2 generation (HyPer probe), reduced-to-oxidized glutathione ratio and NOX2 expression. After eight weeks of HFD, insulin-dependent glucose uptake was impaired in skeletal muscle fibers when compared with control muscle fibers. Insulin-resistant mice showed increased insulin-stimulated H2O2 release and decreased reduced-to-oxidized glutathione ratio (GSH/GSSG). In addition, p47phox and gp91phox (NOX2 subunits) mRNA levels were also high (~3-fold in HFD mice compared to controls), while protein levels were 6.8- and 1.6-fold higher, respectively. Using apocynin (NOX2 inhibitor) during the HFD feeding period, the oxidative intracellular environment was diminished and skeletal muscle insulin-dependent glucose uptake restored. Our results indicate that insulin-resistant mice have increased H2O2 release upon insulin stimulation when compared with control animals, which appears to be mediated by an increase in NOX2 expression.

Idioma originalEnglish
Páginas (desde-hasta)15740-15754
Número de páginas15
PublicaciónInternational Journal of Molecular Sciences
Volumen14
N.º8
DOI
EstadoPublished - 12 ago 2013

Huella dactilar

muscle fibers
diets
insulin
Insulin
fats
High Fat Diet
Nutrition
Oils and fats
mice
Muscle
Fats
NADPH Oxidase
Muscles
Fibers
oxidase
skeletal muscle
Glutathione Disulfide
glucose
Skeletal Muscle Fibers
Glucose

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Citar esto

Espinosa, A., Campos, C., Díaz-Vegas, A., Galgani, J. E., Juretic, N., Osorio-Fuentealba, C., ... Jaimovich, E. (2013). Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet. International Journal of Molecular Sciences, 14(8), 15740-15754. https://doi.org/10.3390/ijms140815740
Espinosa, Alejandra ; Campos, Cristian ; Díaz-Vegas, Alexis ; Galgani, José E. ; Juretic, Nevenka ; Osorio-Fuentealba, César ; Bucarey, José L. ; Tapia, Gladys ; Valenzuela, Rodrigo ; Contreras-Ferrat, Ariel ; Llanos, Paola ; Jaimovich, Enrique. / Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet. En: International Journal of Molecular Sciences. 2013 ; Vol. 14, N.º 8. pp. 15740-15754.
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title = "Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet",
abstract = "Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) produced by NADPH oxidase 2 (NOX2) is involved in signaling pathways triggered by insulin. We evaluated oxidative status in skeletal muscle fibers from insulin-resistant and control mice by determining H2O2 generation (HyPer probe), reduced-to-oxidized glutathione ratio and NOX2 expression. After eight weeks of HFD, insulin-dependent glucose uptake was impaired in skeletal muscle fibers when compared with control muscle fibers. Insulin-resistant mice showed increased insulin-stimulated H2O2 release and decreased reduced-to-oxidized glutathione ratio (GSH/GSSG). In addition, p47phox and gp91phox (NOX2 subunits) mRNA levels were also high (~3-fold in HFD mice compared to controls), while protein levels were 6.8- and 1.6-fold higher, respectively. Using apocynin (NOX2 inhibitor) during the HFD feeding period, the oxidative intracellular environment was diminished and skeletal muscle insulin-dependent glucose uptake restored. Our results indicate that insulin-resistant mice have increased H2O2 release upon insulin stimulation when compared with control animals, which appears to be mediated by an increase in NOX2 expression.",
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Espinosa, A, Campos, C, Díaz-Vegas, A, Galgani, JE, Juretic, N, Osorio-Fuentealba, C, Bucarey, JL, Tapia, G, Valenzuela, R, Contreras-Ferrat, A, Llanos, P & Jaimovich, E 2013, 'Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet', International Journal of Molecular Sciences, vol. 14, n.º 8, pp. 15740-15754. https://doi.org/10.3390/ijms140815740

Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet. / Espinosa, Alejandra; Campos, Cristian; Díaz-Vegas, Alexis; Galgani, José E.; Juretic, Nevenka; Osorio-Fuentealba, César; Bucarey, José L.; Tapia, Gladys; Valenzuela, Rodrigo; Contreras-Ferrat, Ariel; Llanos, Paola; Jaimovich, Enrique.

En: International Journal of Molecular Sciences, Vol. 14, N.º 8, 12.08.2013, p. 15740-15754.

Resultado de la investigación: Article

TY - JOUR

T1 - Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet

AU - Espinosa, Alejandra

AU - Campos, Cristian

AU - Díaz-Vegas, Alexis

AU - Galgani, José E.

AU - Juretic, Nevenka

AU - Osorio-Fuentealba, César

AU - Bucarey, José L.

AU - Tapia, Gladys

AU - Valenzuela, Rodrigo

AU - Contreras-Ferrat, Ariel

AU - Llanos, Paola

AU - Jaimovich, Enrique

PY - 2013/8/12

Y1 - 2013/8/12

N2 - Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) produced by NADPH oxidase 2 (NOX2) is involved in signaling pathways triggered by insulin. We evaluated oxidative status in skeletal muscle fibers from insulin-resistant and control mice by determining H2O2 generation (HyPer probe), reduced-to-oxidized glutathione ratio and NOX2 expression. After eight weeks of HFD, insulin-dependent glucose uptake was impaired in skeletal muscle fibers when compared with control muscle fibers. Insulin-resistant mice showed increased insulin-stimulated H2O2 release and decreased reduced-to-oxidized glutathione ratio (GSH/GSSG). In addition, p47phox and gp91phox (NOX2 subunits) mRNA levels were also high (~3-fold in HFD mice compared to controls), while protein levels were 6.8- and 1.6-fold higher, respectively. Using apocynin (NOX2 inhibitor) during the HFD feeding period, the oxidative intracellular environment was diminished and skeletal muscle insulin-dependent glucose uptake restored. Our results indicate that insulin-resistant mice have increased H2O2 release upon insulin stimulation when compared with control animals, which appears to be mediated by an increase in NOX2 expression.

AB - Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with a high-fat diet (HFD) are a model of insulin resistance. In skeletal muscle, hydrogen peroxide (H2O2) produced by NADPH oxidase 2 (NOX2) is involved in signaling pathways triggered by insulin. We evaluated oxidative status in skeletal muscle fibers from insulin-resistant and control mice by determining H2O2 generation (HyPer probe), reduced-to-oxidized glutathione ratio and NOX2 expression. After eight weeks of HFD, insulin-dependent glucose uptake was impaired in skeletal muscle fibers when compared with control muscle fibers. Insulin-resistant mice showed increased insulin-stimulated H2O2 release and decreased reduced-to-oxidized glutathione ratio (GSH/GSSG). In addition, p47phox and gp91phox (NOX2 subunits) mRNA levels were also high (~3-fold in HFD mice compared to controls), while protein levels were 6.8- and 1.6-fold higher, respectively. Using apocynin (NOX2 inhibitor) during the HFD feeding period, the oxidative intracellular environment was diminished and skeletal muscle insulin-dependent glucose uptake restored. Our results indicate that insulin-resistant mice have increased H2O2 release upon insulin stimulation when compared with control animals, which appears to be mediated by an increase in NOX2 expression.

KW - Apocynin

KW - Insulin resistance

KW - NOX2

KW - Obesity

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U2 - 10.3390/ijms140815740

DO - 10.3390/ijms140815740

M3 - Article

C2 - 23899788

AN - SCOPUS:84881139517

VL - 14

SP - 15740

EP - 15754

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

ER -

Espinosa A, Campos C, Díaz-Vegas A, Galgani JE, Juretic N, Osorio-Fuentealba C y otros. Insulin-dependent H2O2 production is higher in muscle fibers of mice fed with a high-fat diet. International Journal of Molecular Sciences. 2013 ago 12;14(8):15740-15754. https://doi.org/10.3390/ijms140815740