Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis

Jorge Valdes, Inti Pedroso, Raquel Quatrini, Kevin B. Hallberg, Pablo D T Valenzuela, David S. Holmes

Resultado de la investigación: Conference contribution

10 Citas (Scopus)

Resumen

Draft genome sequences of Acidithiobacillus thiooxidans ATCC 19377 and A. caldus ATCC 51756 have been annotated. Bioinformatic analysis of these two new genomes, together with that of A. ferrooxidans ATCC 23270, allows the prediction of metabolic and regulatory models for each species and has provided a unique opportunity to undertake comparative genomic studies of this group of bioleaching bacteria. In this paper, we report preliminary information on metabolic and electron transfer pathways for ten characteristics of the three acidithiobacilli: CO2 fixation, the TCA cycle, sulfur oxidation, sulfur reduction, iron oxidation, iron assimilation, hydrogen oxidation, flagella formation, Che signaling (chemotaxis) and nitrogen fixation. Predicted transcriptional and metabolic interplay between pathways pinpoints potential coordinated responses to environmental signals such as energy source, oxygen and nutrient limitations. The predicted pathway for nitrogen fixation in A. ferrooxidans will be described as an example of such an integrated response. Several responses appear to be especially characteristic of autotrophic microorganisms and may have direct implications for metabolic processes of critical relevance to the understanding of how these microorganisms survive and proliferate in extreme environments, including industrial bioleaching operations.

Idioma originalEnglish
Título de la publicación alojadaBiohydrometallurgy
Páginas439-442
Número de páginas4
Volumen20-21
EstadoPublished - 2007
Evento17th International Biohydrometallurgy Symposium, IBS 2007 - Frankfurt am Main, Germany
Duración: 2 sep 20075 sep 2007

Serie de la publicación

NombreAdvanced Materials Research
Volumen20-21
ISSN (versión impresa)10226680

Other

Other17th International Biohydrometallurgy Symposium, IBS 2007
PaísGermany
CiudadFrankfurt am Main
Período2/09/075/09/07

Huella dactilar

Metabolism
Nitrogen fixation
Bioleaching
Genes
Microorganisms
Oxidation
Sulfur
Iron
Bioinformatics
Nutrients
Bacteria
Hydrogen
Oxygen
Electrons

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Valdes, J., Pedroso, I., Quatrini, R., Hallberg, K. B., Valenzuela, P. D. T., & Holmes, D. S. (2007). Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis. En Biohydrometallurgy (Vol. 20-21, pp. 439-442). (Advanced Materials Research; Vol. 20-21).
Valdes, Jorge ; Pedroso, Inti ; Quatrini, Raquel ; Hallberg, Kevin B. ; Valenzuela, Pablo D T ; Holmes, David S. / Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis. Biohydrometallurgy. Vol. 20-21 2007. pp. 439-442 (Advanced Materials Research).
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abstract = "Draft genome sequences of Acidithiobacillus thiooxidans ATCC 19377 and A. caldus ATCC 51756 have been annotated. Bioinformatic analysis of these two new genomes, together with that of A. ferrooxidans ATCC 23270, allows the prediction of metabolic and regulatory models for each species and has provided a unique opportunity to undertake comparative genomic studies of this group of bioleaching bacteria. In this paper, we report preliminary information on metabolic and electron transfer pathways for ten characteristics of the three acidithiobacilli: CO2 fixation, the TCA cycle, sulfur oxidation, sulfur reduction, iron oxidation, iron assimilation, hydrogen oxidation, flagella formation, Che signaling (chemotaxis) and nitrogen fixation. Predicted transcriptional and metabolic interplay between pathways pinpoints potential coordinated responses to environmental signals such as energy source, oxygen and nutrient limitations. The predicted pathway for nitrogen fixation in A. ferrooxidans will be described as an example of such an integrated response. Several responses appear to be especially characteristic of autotrophic microorganisms and may have direct implications for metabolic processes of critical relevance to the understanding of how these microorganisms survive and proliferate in extreme environments, including industrial bioleaching operations.",
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Valdes, J, Pedroso, I, Quatrini, R, Hallberg, KB, Valenzuela, PDT & Holmes, DS 2007, Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis. En Biohydrometallurgy. vol. 20-21, Advanced Materials Research, vol. 20-21, pp. 439-442, 17th International Biohydrometallurgy Symposium, IBS 2007, Frankfurt am Main, Germany, 2/09/07.

Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis. / Valdes, Jorge; Pedroso, Inti; Quatrini, Raquel; Hallberg, Kevin B.; Valenzuela, Pablo D T; Holmes, David S.

Biohydrometallurgy. Vol. 20-21 2007. p. 439-442 (Advanced Materials Research; Vol. 20-21).

Resultado de la investigación: Conference contribution

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AU - Pedroso, Inti

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AU - Hallberg, Kevin B.

AU - Valenzuela, Pablo D T

AU - Holmes, David S.

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AB - Draft genome sequences of Acidithiobacillus thiooxidans ATCC 19377 and A. caldus ATCC 51756 have been annotated. Bioinformatic analysis of these two new genomes, together with that of A. ferrooxidans ATCC 23270, allows the prediction of metabolic and regulatory models for each species and has provided a unique opportunity to undertake comparative genomic studies of this group of bioleaching bacteria. In this paper, we report preliminary information on metabolic and electron transfer pathways for ten characteristics of the three acidithiobacilli: CO2 fixation, the TCA cycle, sulfur oxidation, sulfur reduction, iron oxidation, iron assimilation, hydrogen oxidation, flagella formation, Che signaling (chemotaxis) and nitrogen fixation. Predicted transcriptional and metabolic interplay between pathways pinpoints potential coordinated responses to environmental signals such as energy source, oxygen and nutrient limitations. The predicted pathway for nitrogen fixation in A. ferrooxidans will be described as an example of such an integrated response. Several responses appear to be especially characteristic of autotrophic microorganisms and may have direct implications for metabolic processes of critical relevance to the understanding of how these microorganisms survive and proliferate in extreme environments, including industrial bioleaching operations.

KW - Acidithiobacillus

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Valdes J, Pedroso I, Quatrini R, Hallberg KB, Valenzuela PDT, Holmes DS. Insights into the metabolism and ecophysiology of three acidithiobacilli by comparative genome analysis. En Biohydrometallurgy. Vol. 20-21. 2007. p. 439-442. (Advanced Materials Research).