Diethyl pyrocarbonate (DEPC) caused a loss in the ability of inactive subunits of wild-type and H141F mutant human liver arginase (EC 18.104.22.168) to be reactivated by Mn2+. The effect was reversed by hydroxylamine and involved a residue with a pKa of 6.5 ± 0.1. Half activation with Mn2+ was sufficient for total resistance of H141F and full activation was not impeded by a previous incubation of the half-active species with DEPC. The H101N and H126N mutants expressed 60 and 82% of the wild-type activity, respectively, without changes in Km for arginine or Ki for lysine inhibition. After dialysis against EDTA, H126N was inactive in the absence of added Mn2+ and contained < 0.1 Mn2+/subunit, whereas H101N was half active and contained 1.2 ± 0.1 Mn2+/subunit. Results support the concept that a weakly bound metal ion is needed only for conversion of active species to a more active active state.
Áreas temáticas de ASJC Scopus
- Biología molecular