Inflammation, synovial angiogenesis and chondroid apoptosis in the evolution of type II collagen-induced arthritis

Carlos González, Paula Abello, Raquel Cepeda, Lorena Salazar, Octavio Aravena, Barbara Pesce, Diego Catalán, Juan C. Aguillón

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

Using the murine model of type II collagen-induced arthritis (CIA), we studied its evolution over time by histopathological, immunohistochemical and clinical evaluations. The first clinical symptoms appeared 28 days post-inoculation (dpi), with bovine type II collagen, with an average arthritic index of 1.00 ± 0.48 corresponding to erythema of the articulation. The disease progressed, and by 70 dpi showed an average arthritic index of 3.83 ± 0.27 corresponding to edema and maximum deformation, with ankylosis. Computed morphometry demonstrated that, in comparison to controls, the induction of CIA, produces a significant and increasing accumulation of inflammatory cells, fibrosis (p < 0.0001) and cartilage destruction (p = 0.0029). Likewise, the area of von Willebrand factor (vWF) immunostaining, as an indicator of endothelial proliferation, increased significantly from 28 dpi (p < 0.0001), in CIA mice compared to controls. However, the effective synovial vascularization, calculated as the synovial vascular bed area index, significantly increased by 42 dpi (p = 0.0014). This indicates that the activation and proliferation of endothelium becomes significant before an effective vascularization area is formed. The apoptosis index was also an earlier indicator of cartilage damage, becoming significant from 28 dpi in comparison to controls (p < 0.0001). Finally, it was observed that the increase in the arthritic index showed a strong correlation with the increase in both angiogenesis (r = 0.95; p = 0.0021) and apoptosis (r = 0.90; p = 0.0015). In conclusion, a robust correlation between synovial membrane inflammation, angiogenesis and chondrocyte apoptosis, with respect to the increase in the clinical severity of CIA, has been demonstrated by a quantitative computer-assisted immunomorphometric analysis.

Idioma originalEnglish
Páginas (desde-hasta)127-135
Número de páginas9
PublicaciónEuropean Cytokine Network
Volumen18
N.º3
DOI
EstadoPublished - sep 2007

Huella dactilar

Experimental Arthritis
Collagen Type II
Collagen
Cartilage
Apoptosis
Inflammation
Arthritis
von Willebrand Factor
Ankylosis
Synovial Membrane
Erythema
Chondrocytes
Chemical activation
Cells
Endothelium
Membranes
Blood Vessels
Edema
Fibrosis

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Immunology and Allergy
  • Clinical Biochemistry

Citar esto

González, Carlos ; Abello, Paula ; Cepeda, Raquel ; Salazar, Lorena ; Aravena, Octavio ; Pesce, Barbara ; Catalán, Diego ; Aguillón, Juan C. / Inflammation, synovial angiogenesis and chondroid apoptosis in the evolution of type II collagen-induced arthritis. En: European Cytokine Network. 2007 ; Vol. 18, N.º 3. pp. 127-135.
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abstract = "Using the murine model of type II collagen-induced arthritis (CIA), we studied its evolution over time by histopathological, immunohistochemical and clinical evaluations. The first clinical symptoms appeared 28 days post-inoculation (dpi), with bovine type II collagen, with an average arthritic index of 1.00 ± 0.48 corresponding to erythema of the articulation. The disease progressed, and by 70 dpi showed an average arthritic index of 3.83 ± 0.27 corresponding to edema and maximum deformation, with ankylosis. Computed morphometry demonstrated that, in comparison to controls, the induction of CIA, produces a significant and increasing accumulation of inflammatory cells, fibrosis (p < 0.0001) and cartilage destruction (p = 0.0029). Likewise, the area of von Willebrand factor (vWF) immunostaining, as an indicator of endothelial proliferation, increased significantly from 28 dpi (p < 0.0001), in CIA mice compared to controls. However, the effective synovial vascularization, calculated as the synovial vascular bed area index, significantly increased by 42 dpi (p = 0.0014). This indicates that the activation and proliferation of endothelium becomes significant before an effective vascularization area is formed. The apoptosis index was also an earlier indicator of cartilage damage, becoming significant from 28 dpi in comparison to controls (p < 0.0001). Finally, it was observed that the increase in the arthritic index showed a strong correlation with the increase in both angiogenesis (r = 0.95; p = 0.0021) and apoptosis (r = 0.90; p = 0.0015). In conclusion, a robust correlation between synovial membrane inflammation, angiogenesis and chondrocyte apoptosis, with respect to the increase in the clinical severity of CIA, has been demonstrated by a quantitative computer-assisted immunomorphometric analysis.",
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González, C, Abello, P, Cepeda, R, Salazar, L, Aravena, O, Pesce, B, Catalán, D & Aguillón, JC 2007, 'Inflammation, synovial angiogenesis and chondroid apoptosis in the evolution of type II collagen-induced arthritis', European Cytokine Network, vol. 18, n.º 3, pp. 127-135. https://doi.org/10.1684/ecn.2007.0099

Inflammation, synovial angiogenesis and chondroid apoptosis in the evolution of type II collagen-induced arthritis. / González, Carlos; Abello, Paula; Cepeda, Raquel; Salazar, Lorena; Aravena, Octavio; Pesce, Barbara; Catalán, Diego; Aguillón, Juan C.

En: European Cytokine Network, Vol. 18, N.º 3, 09.2007, p. 127-135.

Resultado de la investigación: Article

TY - JOUR

T1 - Inflammation, synovial angiogenesis and chondroid apoptosis in the evolution of type II collagen-induced arthritis

AU - González, Carlos

AU - Abello, Paula

AU - Cepeda, Raquel

AU - Salazar, Lorena

AU - Aravena, Octavio

AU - Pesce, Barbara

AU - Catalán, Diego

AU - Aguillón, Juan C.

PY - 2007/9

Y1 - 2007/9

N2 - Using the murine model of type II collagen-induced arthritis (CIA), we studied its evolution over time by histopathological, immunohistochemical and clinical evaluations. The first clinical symptoms appeared 28 days post-inoculation (dpi), with bovine type II collagen, with an average arthritic index of 1.00 ± 0.48 corresponding to erythema of the articulation. The disease progressed, and by 70 dpi showed an average arthritic index of 3.83 ± 0.27 corresponding to edema and maximum deformation, with ankylosis. Computed morphometry demonstrated that, in comparison to controls, the induction of CIA, produces a significant and increasing accumulation of inflammatory cells, fibrosis (p < 0.0001) and cartilage destruction (p = 0.0029). Likewise, the area of von Willebrand factor (vWF) immunostaining, as an indicator of endothelial proliferation, increased significantly from 28 dpi (p < 0.0001), in CIA mice compared to controls. However, the effective synovial vascularization, calculated as the synovial vascular bed area index, significantly increased by 42 dpi (p = 0.0014). This indicates that the activation and proliferation of endothelium becomes significant before an effective vascularization area is formed. The apoptosis index was also an earlier indicator of cartilage damage, becoming significant from 28 dpi in comparison to controls (p < 0.0001). Finally, it was observed that the increase in the arthritic index showed a strong correlation with the increase in both angiogenesis (r = 0.95; p = 0.0021) and apoptosis (r = 0.90; p = 0.0015). In conclusion, a robust correlation between synovial membrane inflammation, angiogenesis and chondrocyte apoptosis, with respect to the increase in the clinical severity of CIA, has been demonstrated by a quantitative computer-assisted immunomorphometric analysis.

AB - Using the murine model of type II collagen-induced arthritis (CIA), we studied its evolution over time by histopathological, immunohistochemical and clinical evaluations. The first clinical symptoms appeared 28 days post-inoculation (dpi), with bovine type II collagen, with an average arthritic index of 1.00 ± 0.48 corresponding to erythema of the articulation. The disease progressed, and by 70 dpi showed an average arthritic index of 3.83 ± 0.27 corresponding to edema and maximum deformation, with ankylosis. Computed morphometry demonstrated that, in comparison to controls, the induction of CIA, produces a significant and increasing accumulation of inflammatory cells, fibrosis (p < 0.0001) and cartilage destruction (p = 0.0029). Likewise, the area of von Willebrand factor (vWF) immunostaining, as an indicator of endothelial proliferation, increased significantly from 28 dpi (p < 0.0001), in CIA mice compared to controls. However, the effective synovial vascularization, calculated as the synovial vascular bed area index, significantly increased by 42 dpi (p = 0.0014). This indicates that the activation and proliferation of endothelium becomes significant before an effective vascularization area is formed. The apoptosis index was also an earlier indicator of cartilage damage, becoming significant from 28 dpi in comparison to controls (p < 0.0001). Finally, it was observed that the increase in the arthritic index showed a strong correlation with the increase in both angiogenesis (r = 0.95; p = 0.0021) and apoptosis (r = 0.90; p = 0.0015). In conclusion, a robust correlation between synovial membrane inflammation, angiogenesis and chondrocyte apoptosis, with respect to the increase in the clinical severity of CIA, has been demonstrated by a quantitative computer-assisted immunomorphometric analysis.

KW - Angiogenesis

KW - Apoptosis

KW - Arthritis

KW - CIA

KW - Morphometric analysis

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