Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation

Harold Oliva, Rodrigo Pacheco, José M. Martinez-Navio, Marta Rodríguez-García, Mar Naranjo-Gómez, Núria Climent, Carolina Prado, Cristina Gil, Montserrat Plana, Felipe García, José M. Miró, Rafael Franco, Francesc E. Borras, Naveenan Navaratnam, José M. Gatell, Teresa Gallart

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

APOBEC3G (apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; A3G) is an innate defense protein showing activity against retroviruses and retrotransposons. Activated CD4+ T cells are highly permissive for HIV-1 replication, whereas resting CD4+ T cells are refractory. Dendritic cells (DCs), especially mature DCs, are also refractory. We investigated whether these differences could be related to a differential A3G expression and/or subcellular distribution. We found that A3G mRNA and protein expression is very low in resting CD4+ T cells and immature DCs, but increases strongly following T-cell activation and DC maturation. The Apo-7 anti-A3G monoclonal antibody (mAb), which was specifically developed, confirmed these differences at the protein level and disclosed that A3G is mainly cytoplasmic in resting CD4+ T cells and immature DCs. Nevertheless, A3G translocates to the nucleus in activated-proliferating CD4+ T cells, yet remaining cytoplasmic in matured DCs, a finding confirmed by immunoblotting analysis of cytoplasmic and nuclear fractions. Apo-7 mAb was able to immunoprecipitate endogenous A3G allowing to detect complexes with numerous proteins in activated-proliferating but not in resting CD4+ T cells. The results show for the first time the nuclear translocation of A3G in activated-proliferating CD4+ T cells.

Idioma originalEnglish
Páginas (desde-hasta)689-700
Número de páginas12
PublicaciónImmunology and Cell Biology
Volumen94
N.º7
DOI
EstadoPublished - 1 ago 2016

Huella dactilar

Cytidine Deaminase
Dendritic Cells
T-Lymphocytes
Proteins
Monoclonal Antibodies
Retroelements
human APOBEC3G protein
Retroviridae
Immunoblotting
HIV-1
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Citar esto

Oliva, H., Pacheco, R., Martinez-Navio, J. M., Rodríguez-García, M., Naranjo-Gómez, M., Climent, N., ... Gallart, T. (2016). Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation. Immunology and Cell Biology, 94(7), 689-700. https://doi.org/10.1038/icb.2016.28
Oliva, Harold ; Pacheco, Rodrigo ; Martinez-Navio, José M. ; Rodríguez-García, Marta ; Naranjo-Gómez, Mar ; Climent, Núria ; Prado, Carolina ; Gil, Cristina ; Plana, Montserrat ; García, Felipe ; Miró, José M. ; Franco, Rafael ; Borras, Francesc E. ; Navaratnam, Naveenan ; Gatell, José M. ; Gallart, Teresa. / Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation. En: Immunology and Cell Biology. 2016 ; Vol. 94, N.º 7. pp. 689-700.
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title = "Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation",
abstract = "APOBEC3G (apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; A3G) is an innate defense protein showing activity against retroviruses and retrotransposons. Activated CD4+ T cells are highly permissive for HIV-1 replication, whereas resting CD4+ T cells are refractory. Dendritic cells (DCs), especially mature DCs, are also refractory. We investigated whether these differences could be related to a differential A3G expression and/or subcellular distribution. We found that A3G mRNA and protein expression is very low in resting CD4+ T cells and immature DCs, but increases strongly following T-cell activation and DC maturation. The Apo-7 anti-A3G monoclonal antibody (mAb), which was specifically developed, confirmed these differences at the protein level and disclosed that A3G is mainly cytoplasmic in resting CD4+ T cells and immature DCs. Nevertheless, A3G translocates to the nucleus in activated-proliferating CD4+ T cells, yet remaining cytoplasmic in matured DCs, a finding confirmed by immunoblotting analysis of cytoplasmic and nuclear fractions. Apo-7 mAb was able to immunoprecipitate endogenous A3G allowing to detect complexes with numerous proteins in activated-proliferating but not in resting CD4+ T cells. The results show for the first time the nuclear translocation of A3G in activated-proliferating CD4+ T cells.",
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Oliva, H, Pacheco, R, Martinez-Navio, JM, Rodríguez-García, M, Naranjo-Gómez, M, Climent, N, Prado, C, Gil, C, Plana, M, García, F, Miró, JM, Franco, R, Borras, FE, Navaratnam, N, Gatell, JM & Gallart, T 2016, 'Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation', Immunology and Cell Biology, vol. 94, n.º 7, pp. 689-700. https://doi.org/10.1038/icb.2016.28

Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation. / Oliva, Harold; Pacheco, Rodrigo; Martinez-Navio, José M.; Rodríguez-García, Marta; Naranjo-Gómez, Mar; Climent, Núria; Prado, Carolina; Gil, Cristina; Plana, Montserrat; García, Felipe; Miró, José M.; Franco, Rafael; Borras, Francesc E.; Navaratnam, Naveenan; Gatell, José M.; Gallart, Teresa.

En: Immunology and Cell Biology, Vol. 94, N.º 7, 01.08.2016, p. 689-700.

Resultado de la investigación: Article

TY - JOUR

T1 - Increased expression with differential subcellular location of cytidine deaminase APOBEC3G in human CD4+ T-cell activation and dendritic cell maturation

AU - Oliva, Harold

AU - Pacheco, Rodrigo

AU - Martinez-Navio, José M.

AU - Rodríguez-García, Marta

AU - Naranjo-Gómez, Mar

AU - Climent, Núria

AU - Prado, Carolina

AU - Gil, Cristina

AU - Plana, Montserrat

AU - García, Felipe

AU - Miró, José M.

AU - Franco, Rafael

AU - Borras, Francesc E.

AU - Navaratnam, Naveenan

AU - Gatell, José M.

AU - Gallart, Teresa

PY - 2016/8/1

Y1 - 2016/8/1

N2 - APOBEC3G (apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; A3G) is an innate defense protein showing activity against retroviruses and retrotransposons. Activated CD4+ T cells are highly permissive for HIV-1 replication, whereas resting CD4+ T cells are refractory. Dendritic cells (DCs), especially mature DCs, are also refractory. We investigated whether these differences could be related to a differential A3G expression and/or subcellular distribution. We found that A3G mRNA and protein expression is very low in resting CD4+ T cells and immature DCs, but increases strongly following T-cell activation and DC maturation. The Apo-7 anti-A3G monoclonal antibody (mAb), which was specifically developed, confirmed these differences at the protein level and disclosed that A3G is mainly cytoplasmic in resting CD4+ T cells and immature DCs. Nevertheless, A3G translocates to the nucleus in activated-proliferating CD4+ T cells, yet remaining cytoplasmic in matured DCs, a finding confirmed by immunoblotting analysis of cytoplasmic and nuclear fractions. Apo-7 mAb was able to immunoprecipitate endogenous A3G allowing to detect complexes with numerous proteins in activated-proliferating but not in resting CD4+ T cells. The results show for the first time the nuclear translocation of A3G in activated-proliferating CD4+ T cells.

AB - APOBEC3G (apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; A3G) is an innate defense protein showing activity against retroviruses and retrotransposons. Activated CD4+ T cells are highly permissive for HIV-1 replication, whereas resting CD4+ T cells are refractory. Dendritic cells (DCs), especially mature DCs, are also refractory. We investigated whether these differences could be related to a differential A3G expression and/or subcellular distribution. We found that A3G mRNA and protein expression is very low in resting CD4+ T cells and immature DCs, but increases strongly following T-cell activation and DC maturation. The Apo-7 anti-A3G monoclonal antibody (mAb), which was specifically developed, confirmed these differences at the protein level and disclosed that A3G is mainly cytoplasmic in resting CD4+ T cells and immature DCs. Nevertheless, A3G translocates to the nucleus in activated-proliferating CD4+ T cells, yet remaining cytoplasmic in matured DCs, a finding confirmed by immunoblotting analysis of cytoplasmic and nuclear fractions. Apo-7 mAb was able to immunoprecipitate endogenous A3G allowing to detect complexes with numerous proteins in activated-proliferating but not in resting CD4+ T cells. The results show for the first time the nuclear translocation of A3G in activated-proliferating CD4+ T cells.

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