Inclusion complexes of estrone and estradiol with β-cyclodextrin: Voltammetric and HPLC studies

Claudia Yañez, Julio Basualdo, Paola Jara-Ulloa, J. Arturo Squella

Resultado de la investigación: Article

16 Citas (Scopus)

Resumen

The interaction of estrone and estradiol with β-cyclodextrins (βCD) was investigated by differential pulse voltammetry (DPV) and high-performance liquid chromatography (HPLC) in mixed media. The co-solvent influence on the tendency of these estrogens to form inclusion complexes with βCD was examined. Thus, acetonitrile (MeCN) and ethanol (EtOH) were used in a mixed aqueous medium containing phosphate buffer. The association constant of the inclusion complexes (Ka) of estrone and estradiol with βCD were determined in two different media by using both voltammetric and Chromatographic techniques. Estradiol was found to bind to βCD with higher affinities than estrone, irrespective of the medium. We have also found a clear influence of the co-solvent on the Ka value, which means a competition of co-solvent molecules with estrogens for binding to the cavity of βCD. Consequently, interaction between βCD and the steroids is weakened when acetonitrile is used.

Idioma originalEnglish
Páginas (desde-hasta)499-505
Número de páginas7
PublicaciónJournal of Physical Organic Chemistry
Volumen20
N.º7
DOI
EstadoPublished - jul 2007

Huella dactilar

Estrone
liquid chromatography
Cyclodextrins
High performance liquid chromatography
Estradiol
inclusions
estrogens
acetonitrile
steroids
Estrogens
affinity
phosphates
tendencies
ethyl alcohol
buffers
interactions
Voltammetry
cavities
Buffers
Ethanol

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry

Citar esto

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abstract = "The interaction of estrone and estradiol with β-cyclodextrins (βCD) was investigated by differential pulse voltammetry (DPV) and high-performance liquid chromatography (HPLC) in mixed media. The co-solvent influence on the tendency of these estrogens to form inclusion complexes with βCD was examined. Thus, acetonitrile (MeCN) and ethanol (EtOH) were used in a mixed aqueous medium containing phosphate buffer. The association constant of the inclusion complexes (Ka) of estrone and estradiol with βCD were determined in two different media by using both voltammetric and Chromatographic techniques. Estradiol was found to bind to βCD with higher affinities than estrone, irrespective of the medium. We have also found a clear influence of the co-solvent on the Ka value, which means a competition of co-solvent molecules with estrogens for binding to the cavity of βCD. Consequently, interaction between βCD and the steroids is weakened when acetonitrile is used.",
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Inclusion complexes of estrone and estradiol with β-cyclodextrin : Voltammetric and HPLC studies. / Yañez, Claudia; Basualdo, Julio; Jara-Ulloa, Paola; Squella, J. Arturo.

En: Journal of Physical Organic Chemistry, Vol. 20, N.º 7, 07.2007, p. 499-505.

Resultado de la investigación: Article

TY - JOUR

T1 - Inclusion complexes of estrone and estradiol with β-cyclodextrin

T2 - Voltammetric and HPLC studies

AU - Yañez, Claudia

AU - Basualdo, Julio

AU - Jara-Ulloa, Paola

AU - Squella, J. Arturo

PY - 2007/7

Y1 - 2007/7

N2 - The interaction of estrone and estradiol with β-cyclodextrins (βCD) was investigated by differential pulse voltammetry (DPV) and high-performance liquid chromatography (HPLC) in mixed media. The co-solvent influence on the tendency of these estrogens to form inclusion complexes with βCD was examined. Thus, acetonitrile (MeCN) and ethanol (EtOH) were used in a mixed aqueous medium containing phosphate buffer. The association constant of the inclusion complexes (Ka) of estrone and estradiol with βCD were determined in two different media by using both voltammetric and Chromatographic techniques. Estradiol was found to bind to βCD with higher affinities than estrone, irrespective of the medium. We have also found a clear influence of the co-solvent on the Ka value, which means a competition of co-solvent molecules with estrogens for binding to the cavity of βCD. Consequently, interaction between βCD and the steroids is weakened when acetonitrile is used.

AB - The interaction of estrone and estradiol with β-cyclodextrins (βCD) was investigated by differential pulse voltammetry (DPV) and high-performance liquid chromatography (HPLC) in mixed media. The co-solvent influence on the tendency of these estrogens to form inclusion complexes with βCD was examined. Thus, acetonitrile (MeCN) and ethanol (EtOH) were used in a mixed aqueous medium containing phosphate buffer. The association constant of the inclusion complexes (Ka) of estrone and estradiol with βCD were determined in two different media by using both voltammetric and Chromatographic techniques. Estradiol was found to bind to βCD with higher affinities than estrone, irrespective of the medium. We have also found a clear influence of the co-solvent on the Ka value, which means a competition of co-solvent molecules with estrogens for binding to the cavity of βCD. Consequently, interaction between βCD and the steroids is weakened when acetonitrile is used.

KW - β-cyclodextrin

KW - Inclusion complexes

KW - Steroids

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