Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi

T. C. Zahrt, G. C. Mora, S. Maloy

Resultado de la investigación: Comment/debate

46 Citas (Scopus)

Resumen

P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.

Idioma originalEnglish
Páginas (desde-hasta)1527-1529
Número de páginas3
PublicaciónJournal of Bacteriology
Volumen176
N.º5
EstadoPublished - 1994

Huella dactilar

Salmonella typhi
DNA Mismatch Repair
Salmonella typhimurium
Genes
DNA
DNA Restriction Enzymes
Plasmids

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Molecular Biology

Citar esto

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title = "Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi",
abstract = "P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.",
author = "Zahrt, {T. C.} and Mora, {G. C.} and S. Maloy",
year = "1994",
language = "English",
volume = "176",
pages = "1527--1529",
journal = "Journal of Bacteriology",
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Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi. / Zahrt, T. C.; Mora, G. C.; Maloy, S.

En: Journal of Bacteriology, Vol. 176, N.º 5, 1994, p. 1527-1529.

Resultado de la investigación: Comment/debate

TY - JOUR

T1 - Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi

AU - Zahrt, T. C.

AU - Mora, G. C.

AU - Maloy, S.

PY - 1994

Y1 - 1994

N2 - P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.

AB - P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.

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M3 - Comment/debate

C2 - 8113197

AN - SCOPUS:0028204784

VL - 176

SP - 1527

EP - 1529

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 5

ER -