Resumen
P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 1527-1529 |
| Número de páginas | 3 |
| Publicación | Journal of Bacteriology |
| Volumen | 176 |
| N.º | 5 |
| Estado | Published - 1994 |
Huella dactilar
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
- Microbiology
- Molecular Biology
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Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi. / Zahrt, T. C.; Mora, G. C.; Maloy, S.
En: Journal of Bacteriology, Vol. 176, N.º 5, 1994, p. 1527-1529.Resultado de la investigación: Comment/debate
TY - JOUR
T1 - Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi
AU - Zahrt, T. C.
AU - Mora, G. C.
AU - Maloy, S.
PY - 1994
Y1 - 1994
N2 - P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.
AB - P22 transduction of chromosomal genes from Salmonella typhimurium into Salmonella typhi occurs at a low frequency. Transduction of plasmids from S. typhimurium into S. typhi occurs at a frequency similar to that between S. typhimurium strains, indicating that the barrier to transduction of chromosomal genes is not due to an inability of P22 to inject DNA into S. typhi or a restriction endonuclease that rapidly degrades foreign DNA. Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently. These results indicate that the transduction barrier is due to activity of the recipient mismatch repair system, which senses sequence divergence and disrupts heteroduplexes in favor of recipient sequences. Inactivation of the mismatch repair system allows P22 transduction to be used as an effective tool for constructing S. typhi-S. typhimurium hybrids.
UR - http://www.scopus.com/inward/record.url?scp=0028204784&partnerID=8YFLogxK
M3 - Comment/debate
C2 - 8113197
AN - SCOPUS:0028204784
VL - 176
SP - 1527
EP - 1529
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 5
ER -