In vivo subunit hybridization of succinic semialdehyde and 4-aminobutanal dehydrogenases from a pseudomonas species

Mario S. Rosemblatt, Denis M. Callewaert, T. T. Tchen

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Two minor NAD+-specific aldehyde dehydrogenase fractions have been isolated from a Pseudomonas species grown on putrescine as the sole source of C and N. The chromatographic, enzymic, and electrophoretic behavior of these two fractions indicate that they are hybrids at the subunit level of two trimeric enzymes (succinic semialdehyde dehydrogenase and 4-aminobutanal dehydrogenase) previously purified from this organism. The molecular weights of these two fractions agree with the theoretical values for the hybrids. Immunodiffusion and inhibition of activity experiments conducted with antibodies to the two purified enzymes show antigenic similarities between either of the purified enzymes and the hybrids, but not between the purified enzymes themselves. Finally, electrophoresis in the presence of sodium dodecyl sulfate of the immunoprecipitate of one of the hybrids reveals the presence of subunits of both of the pure dehydrogenases. These results represent an unusual situation with no known precedent and indicate an evolutionary relationship between these enzymes.

Idioma originalEnglish
Páginas (desde-hasta)4176-4180
Número de páginas5
PublicaciónBiochemistry
Volumen13
N.º20
DOI
EstadoPublished - 1 sep 1974

Huella dactilar

aminobutyraldehyde dehydrogenase
Pseudomonas
Enzymes
Succinate-Semialdehyde Dehydrogenase
Aldehyde Dehydrogenase
Putrescine
Immunodiffusion
Electrophoresis
Sodium Dodecyl Sulfate
NAD
Oxidoreductases
Molecular Weight
Molecular weight
succinic semialdehyde
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Citar esto

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title = "In vivo subunit hybridization of succinic semialdehyde and 4-aminobutanal dehydrogenases from a pseudomonas species",
abstract = "Two minor NAD+-specific aldehyde dehydrogenase fractions have been isolated from a Pseudomonas species grown on putrescine as the sole source of C and N. The chromatographic, enzymic, and electrophoretic behavior of these two fractions indicate that they are hybrids at the subunit level of two trimeric enzymes (succinic semialdehyde dehydrogenase and 4-aminobutanal dehydrogenase) previously purified from this organism. The molecular weights of these two fractions agree with the theoretical values for the hybrids. Immunodiffusion and inhibition of activity experiments conducted with antibodies to the two purified enzymes show antigenic similarities between either of the purified enzymes and the hybrids, but not between the purified enzymes themselves. Finally, electrophoresis in the presence of sodium dodecyl sulfate of the immunoprecipitate of one of the hybrids reveals the presence of subunits of both of the pure dehydrogenases. These results represent an unusual situation with no known precedent and indicate an evolutionary relationship between these enzymes.",
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In vivo subunit hybridization of succinic semialdehyde and 4-aminobutanal dehydrogenases from a pseudomonas species. / Rosemblatt, Mario S.; Callewaert, Denis M.; Tchen, T. T.

En: Biochemistry, Vol. 13, N.º 20, 01.09.1974, p. 4176-4180.

Resultado de la investigación: Article

TY - JOUR

T1 - In vivo subunit hybridization of succinic semialdehyde and 4-aminobutanal dehydrogenases from a pseudomonas species

AU - Rosemblatt, Mario S.

AU - Callewaert, Denis M.

AU - Tchen, T. T.

PY - 1974/9/1

Y1 - 1974/9/1

N2 - Two minor NAD+-specific aldehyde dehydrogenase fractions have been isolated from a Pseudomonas species grown on putrescine as the sole source of C and N. The chromatographic, enzymic, and electrophoretic behavior of these two fractions indicate that they are hybrids at the subunit level of two trimeric enzymes (succinic semialdehyde dehydrogenase and 4-aminobutanal dehydrogenase) previously purified from this organism. The molecular weights of these two fractions agree with the theoretical values for the hybrids. Immunodiffusion and inhibition of activity experiments conducted with antibodies to the two purified enzymes show antigenic similarities between either of the purified enzymes and the hybrids, but not between the purified enzymes themselves. Finally, electrophoresis in the presence of sodium dodecyl sulfate of the immunoprecipitate of one of the hybrids reveals the presence of subunits of both of the pure dehydrogenases. These results represent an unusual situation with no known precedent and indicate an evolutionary relationship between these enzymes.

AB - Two minor NAD+-specific aldehyde dehydrogenase fractions have been isolated from a Pseudomonas species grown on putrescine as the sole source of C and N. The chromatographic, enzymic, and electrophoretic behavior of these two fractions indicate that they are hybrids at the subunit level of two trimeric enzymes (succinic semialdehyde dehydrogenase and 4-aminobutanal dehydrogenase) previously purified from this organism. The molecular weights of these two fractions agree with the theoretical values for the hybrids. Immunodiffusion and inhibition of activity experiments conducted with antibodies to the two purified enzymes show antigenic similarities between either of the purified enzymes and the hybrids, but not between the purified enzymes themselves. Finally, electrophoresis in the presence of sodium dodecyl sulfate of the immunoprecipitate of one of the hybrids reveals the presence of subunits of both of the pure dehydrogenases. These results represent an unusual situation with no known precedent and indicate an evolutionary relationship between these enzymes.

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U2 - 10.1021/bi00717a017

DO - 10.1021/bi00717a017

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VL - 13

SP - 4176

EP - 4180

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

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