In vivo expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA

Mariana Rios, Alejandro Venegas, Horacio B. Croxatto

Resultado de la investigación: Review article

2 Citas (Scopus)

Resumen

Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (Ríos et al., 1997). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 μg of pure β-galactosidase (β-gal) mRNA, 50 μg of pure DNA from the reporter gene β-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the β-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-β-D-galactopyranoside as a substrate. The administration of β-gal mRNA and pSVBgal plasmid increased β-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for β-gal can enter oviductal cells and he translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA.

Idioma originalEnglish
Páginas (desde-hasta)333-338
Número de páginas6
PublicaciónBiological Research
Volumen35
N.º3-4
EstadoPublished - 2002

Huella dactilar

Galactosidases
galactosidases
Oviducts
messenger RNA
oviducts
Rats
Messenger RNA
DNA
rats
estradiol
Estradiol
Ovum
RNA
Galactose
Reporter Genes
reporter genes
translation (genetics)
plasmids
Plasmids
Embryonic Structures

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Citar esto

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title = "In vivo expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA",
abstract = "Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (R{\'i}os et al., 1997). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 μg of pure β-galactosidase (β-gal) mRNA, 50 μg of pure DNA from the reporter gene β-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the β-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-β-D-galactopyranoside as a substrate. The administration of β-gal mRNA and pSVBgal plasmid increased β-gal activity by 71{\%} and 142{\%}, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for β-gal can enter oviductal cells and he translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA.",
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journal = "Biological Research",
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In vivo expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA. / Rios, Mariana; Venegas, Alejandro; Croxatto, Horacio B.

En: Biological Research, Vol. 35, N.º 3-4, 2002, p. 333-338.

Resultado de la investigación: Review article

TY - JOUR

T1 - In vivo expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA

AU - Rios, Mariana

AU - Venegas, Alejandro

AU - Croxatto, Horacio B.

PY - 2002

Y1 - 2002

N2 - Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (Ríos et al., 1997). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 μg of pure β-galactosidase (β-gal) mRNA, 50 μg of pure DNA from the reporter gene β-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the β-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-β-D-galactopyranoside as a substrate. The administration of β-gal mRNA and pSVBgal plasmid increased β-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for β-gal can enter oviductal cells and he translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA.

AB - Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (Ríos et al., 1997). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 μg of pure β-galactosidase (β-gal) mRNA, 50 μg of pure DNA from the reporter gene β-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the β-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-β-D-galactopyranoside as a substrate. The administration of β-gal mRNA and pSVBgal plasmid increased β-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for β-gal can enter oviductal cells and he translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA.

KW - β-galactosidase

KW - Gene transfer

KW - Oviduct

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M3 - Review article

C2 - 12462985

AN - SCOPUS:1842843989

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EP - 338

JO - Biological Research

JF - Biological Research

SN - 0716-9760

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