In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics

Viviana R. Ordenes, Ignacio Moreno, Daniel Maturana, Lorena Norambuena, Anthony J. Trewavas, Ariel Orellana

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

The Golgi apparatus is thought to play a role in calcium homeostasis in plant cells. However, the calcium dynamics in this organelle is unknown in plants. To monitor the [Ca2+]Golgi in vivo, we obtained and analyzed Arabidopsis thaliana plants that express aequorin in the Golgi. Our results show that free [Ca2+] levels in the Golgi are higher than in the cytosol (0.70μM vs. 0.05μM, respectively). Stimuli such as cold shock, mechanical stimulation and hyperosmotic stress, led to a transient increase in cytosolic calcium; however, no instant change in the [Ca2+]Golgi concentration was detected. Nevertheless, a delayed increase in the [Ca2+]Golgi up to 2-3μM was observed. Cyclopiazonic acid and thapsigargin inhibited the stimuli-induced [Ca2+]Golgi increase, suggesting that [Ca2+]Golgi levels are dependent upon the activity of Ca2+-ATPases. Treatment of these plants with the synthetic auxin analog, 2,4-dichlorophenoxy acetic acid (2,4-D), produced a slow decrease of free calcium in the organelle. Our results indicate that the plant Golgi apparatus is not involved in the generation of cytosolic calcium transients and exhibits its own dynamics modulated in part by the activity of Ca2+ pumps and hormones.

Idioma originalEnglish
Páginas (desde-hasta)397-404
Número de páginas8
PublicaciónCell Calcium
Volumen52
N.º5
DOI
EstadoPublished - 2012

Huella dactilar

Golgi Apparatus
Calcium
2,4-Dichlorophenoxyacetic Acid
Organelles
Aequorin
Indoleacetic Acids
Thapsigargin
Calcium-Transporting ATPases
Plant Cells
Arabidopsis
Cytosol
Shock
Homeostasis
Hormones

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Citar esto

Ordenes, Viviana R. ; Moreno, Ignacio ; Maturana, Daniel ; Norambuena, Lorena ; Trewavas, Anthony J. ; Orellana, Ariel. / In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics. En: Cell Calcium. 2012 ; Vol. 52, N.º 5. pp. 397-404.
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abstract = "The Golgi apparatus is thought to play a role in calcium homeostasis in plant cells. However, the calcium dynamics in this organelle is unknown in plants. To monitor the [Ca2+]Golgi in vivo, we obtained and analyzed Arabidopsis thaliana plants that express aequorin in the Golgi. Our results show that free [Ca2+] levels in the Golgi are higher than in the cytosol (0.70μM vs. 0.05μM, respectively). Stimuli such as cold shock, mechanical stimulation and hyperosmotic stress, led to a transient increase in cytosolic calcium; however, no instant change in the [Ca2+]Golgi concentration was detected. Nevertheless, a delayed increase in the [Ca2+]Golgi up to 2-3μM was observed. Cyclopiazonic acid and thapsigargin inhibited the stimuli-induced [Ca2+]Golgi increase, suggesting that [Ca2+]Golgi levels are dependent upon the activity of Ca2+-ATPases. Treatment of these plants with the synthetic auxin analog, 2,4-dichlorophenoxy acetic acid (2,4-D), produced a slow decrease of free calcium in the organelle. Our results indicate that the plant Golgi apparatus is not involved in the generation of cytosolic calcium transients and exhibits its own dynamics modulated in part by the activity of Ca2+ pumps and hormones.",
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In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics. / Ordenes, Viviana R.; Moreno, Ignacio; Maturana, Daniel; Norambuena, Lorena; Trewavas, Anthony J.; Orellana, Ariel.

En: Cell Calcium, Vol. 52, N.º 5, 2012, p. 397-404.

Resultado de la investigación: Article

TY - JOUR

T1 - In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics

AU - Ordenes, Viviana R.

AU - Moreno, Ignacio

AU - Maturana, Daniel

AU - Norambuena, Lorena

AU - Trewavas, Anthony J.

AU - Orellana, Ariel

PY - 2012

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