In vitro irradiation of colorectal cancer cells by pulsed radiation emitted from a hundred joules plasma focus device and its comparison with continuous irradiation

J. Jain, J. Moreno, R. Andaur, R. Armisen, G. Avaria, B. Bora, S. Davis, C. Pavez, K. Marcelain, L. Soto

Resultado de la investigación: Conference article

1 Cita (Scopus)

Resumen

In the last years, pulsed reduced low dose radiation has been proposed as an alternative for treatment of recurrent cancer. Nonetheless, distinction between the effects of low dose pulsed and continuous radiation is barely known at cellular level. In order to study the effects of low dose pulsed radiation at cellular level, in vitro experiments are important to further advance the basic understanding in this area. In the present work we demonstrate the usefulness of a low-energy plasma focus device PF-400J as a potential source of low-dose pulsed radiation for in vitro cancer cell experiments. Colorectal cancer cell line, DLD-1, were irradiated by pulsed x-rays. Fifty pulses of x-rays provide ∼0.12 Gy dosis, which were measured using thermoluminescence detectors (TLD-100 dosimeters). Irradiation-induced DNA damage was assessed at different time points after irradiation. A statistically significant double strand break (DSB) DNA damage was observed at 30 minutes after irradiation. A comparison of DSB induced by continuous source in the same type cancer cells and pulsed irradiation is made at 30 minutes post-irradiation. In the case of pulsed irradiation, DSB per unit dose found higher. Our findings suggest that low-energy plasma focus devices could have potential application as pulsed radiation source in the area of in vitro cancer cell experiments.

Idioma originalEnglish
Número de artículo012047
PublicaciónJournal of Physics: Conference Series
Volumen1043
N.º1
DOI
EstadoPublished - 25 jun 2018
Evento20th Chilean Physics Symposium - Santiago, Chile
Duración: 30 nov 20162 dic 2016

Huella dactilar

pulsed radiation
plasma focus
cancer
irradiation
dosage
strands
deoxyribonucleic acid
damage
thermoluminescence
radiation sources
cultured cells
dosimeters
continuous radiation
x rays
energy
detectors
radiation
pulses

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Citar esto

Jain, J. ; Moreno, J. ; Andaur, R. ; Armisen, R. ; Avaria, G. ; Bora, B. ; Davis, S. ; Pavez, C. ; Marcelain, K. ; Soto, L. / In vitro irradiation of colorectal cancer cells by pulsed radiation emitted from a hundred joules plasma focus device and its comparison with continuous irradiation. En: Journal of Physics: Conference Series. 2018 ; Vol. 1043, N.º 1.
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abstract = "In the last years, pulsed reduced low dose radiation has been proposed as an alternative for treatment of recurrent cancer. Nonetheless, distinction between the effects of low dose pulsed and continuous radiation is barely known at cellular level. In order to study the effects of low dose pulsed radiation at cellular level, in vitro experiments are important to further advance the basic understanding in this area. In the present work we demonstrate the usefulness of a low-energy plasma focus device PF-400J as a potential source of low-dose pulsed radiation for in vitro cancer cell experiments. Colorectal cancer cell line, DLD-1, were irradiated by pulsed x-rays. Fifty pulses of x-rays provide ∼0.12 Gy dosis, which were measured using thermoluminescence detectors (TLD-100 dosimeters). Irradiation-induced DNA damage was assessed at different time points after irradiation. A statistically significant double strand break (DSB) DNA damage was observed at 30 minutes after irradiation. A comparison of DSB induced by continuous source in the same type cancer cells and pulsed irradiation is made at 30 minutes post-irradiation. In the case of pulsed irradiation, DSB per unit dose found higher. Our findings suggest that low-energy plasma focus devices could have potential application as pulsed radiation source in the area of in vitro cancer cell experiments.",
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In vitro irradiation of colorectal cancer cells by pulsed radiation emitted from a hundred joules plasma focus device and its comparison with continuous irradiation. / Jain, J.; Moreno, J.; Andaur, R.; Armisen, R.; Avaria, G.; Bora, B.; Davis, S.; Pavez, C.; Marcelain, K.; Soto, L.

En: Journal of Physics: Conference Series, Vol. 1043, N.º 1, 012047, 25.06.2018.

Resultado de la investigación: Conference article

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T1 - In vitro irradiation of colorectal cancer cells by pulsed radiation emitted from a hundred joules plasma focus device and its comparison with continuous irradiation

AU - Jain, J.

AU - Moreno, J.

AU - Andaur, R.

AU - Armisen, R.

AU - Avaria, G.

AU - Bora, B.

AU - Davis, S.

AU - Pavez, C.

AU - Marcelain, K.

AU - Soto, L.

PY - 2018/6/25

Y1 - 2018/6/25

N2 - In the last years, pulsed reduced low dose radiation has been proposed as an alternative for treatment of recurrent cancer. Nonetheless, distinction between the effects of low dose pulsed and continuous radiation is barely known at cellular level. In order to study the effects of low dose pulsed radiation at cellular level, in vitro experiments are important to further advance the basic understanding in this area. In the present work we demonstrate the usefulness of a low-energy plasma focus device PF-400J as a potential source of low-dose pulsed radiation for in vitro cancer cell experiments. Colorectal cancer cell line, DLD-1, were irradiated by pulsed x-rays. Fifty pulses of x-rays provide ∼0.12 Gy dosis, which were measured using thermoluminescence detectors (TLD-100 dosimeters). Irradiation-induced DNA damage was assessed at different time points after irradiation. A statistically significant double strand break (DSB) DNA damage was observed at 30 minutes after irradiation. A comparison of DSB induced by continuous source in the same type cancer cells and pulsed irradiation is made at 30 minutes post-irradiation. In the case of pulsed irradiation, DSB per unit dose found higher. Our findings suggest that low-energy plasma focus devices could have potential application as pulsed radiation source in the area of in vitro cancer cell experiments.

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