@inbook{c0f05407ff1b4cf7a0258425fd17add1,
title = "In situ nuclear organization of regulatory machinery",
abstract = "Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",
keywords = "Cell synchrony, Confocal microscopy, FRAP, Immunofluorescence microscopy, Live cell microscopy, Nuclear matrix, Nuclear organization, Runx",
author = "Pockwinse, {Shirwin M.} and Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffery A.} and Amjad Javed and Martin Montecino and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",
year = "2008",
doi = "10.1007/978-1-59745-104-8_17",
language = "English",
isbn = "9781588298287",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "239--259",
booktitle = "Osteoporosis",
}